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A natural skin anti-ageing serum containing pseudobulb ethanolic extract of Grammatophyllum speciosum : a randomized double-blind, placebo-controlled trial / V. Chowjarean in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 41, N° 6 (12/2019)
[article]
Titre : A natural skin anti-ageing serum containing pseudobulb ethanolic extract of Grammatophyllum speciosum : a randomized double-blind, placebo-controlled trial Type de document : texte imprimé Auteurs : V. Chowjarean, Auteur ; P. P. Phiboonchaiyanan, Auteur ; S. Harikarnpakdee, Auteur ; Parkpoom Tengamnuay, Auteur Année de publication : 2019 Article en page(s) : p. 548-557 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Analyse quantitative (chimie)
Antiâge:Antirides
Antioxydants
Biomolécules actives
Cellules -- Cultures et milieux de culture
Dermo-cosmétologie
Etudes cliniques
Peau -- Soins et hygièneIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Grammatophyllum speciosum is utilized to treat sore throats and bronchitis In Thai folk medicine. This study evaluated the in vitro activity and clinical efficacy of a G. speciosum pseudobulb decoction.
- Methods : Measure of in vitro anti-ageing activity was performed using non-cell based assays as well as in CRL 2097 human fibroblast cells. A prophetic patch test method was used to determine skin irritation in 24 healthy Thai volunteers. A randomized double-blind, placebo-controlled trial was conducted with 24 subjects for 56 days after facial application to evaluate efficacy. The results were measured with Visioface® and Cutometer® MPA 580 as well as by visual observations.
- Results : The total content of the antioxidant polyphenols in G. speciosum ethanolic extract (GSE) was 48.19 ± 0.39 mg EGCG equivalent per gram. The GSE possessed potent and higher anti-elastase activity more than EGCG. The extract was able to protect human fibroblasts against superoxide anion-induced cell death at the concentration of 10 µg mL-1. In a clinical study, facial application of the serum containing 0.5% GSE was found to safely increase skin distensibility in healthy volunteers. Skin viscoelasticity and wrinkle volume were also significantly reduced (P < 0.05).
- Conclusion : Thus, both the in vitro and the clinical studies have illustrated the anti-wrinkle/anti-ageing benefits of GSE on human skin.Note de contenu : - MATERIALS AND METHODS : Plant material preparation of G. speciosum pseudobulb extract - Quality control of G. speciosum bulb extract - Non-cell-based assays - Cell-based assay in skin fibroblast cellline - Stability and physicochemicl characteristic of the G. speciosum - Stability of serum containing G. speciosum extract - Clinical trial testing - Sensory and visual assessment - Statistical analysis DOI : 10.1111/ics.12571 Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33663
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Code-barres Cote Support Localisation Section Disponibilité 21409 - Périodique Bibliothèque principale Documentaires Disponible New ingredient from Rosa rugosa delays skin ageing / Annalisa Tito in PERSONAL CARE EUROPE, Vol. 14, N° 2 (04/2020)
[article]
Titre : New ingredient from Rosa rugosa delays skin ageing Type de document : texte imprimé Auteurs : Annalisa Tito, Auteur ; Claudia Zappelli, Auteur ; Maura Angelillo, Auteur ; Maria Gabriella Colucci, Auteur ; Fabio Apone, Auteur Année de publication : 2020 Article en page(s) : p. 131-137 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antiâge
Caractérisation
Cellules -- Cultures et milieux de culture
Chimie analytique
Cosmétiques
Elisa, TestLe test ELISA est une méthode de laboratoire qualifiée d'immuno-enzymatique. Il s'agit d'un test immunologique, car il a pour principal objectif de mettre en évidence la présence d'anticorps ou d'antigènes spécifiques à une pathologie, dans un échantillon de sang. Cet examen utilise une protéine appelée enzyme, qui va se fixer à certains constituants spécifiques de la maladie, et par repérage et quantification de cette enzyme, la maladie pourra être affirmée, ainsi que son intensité évaluée.
Expression génique
Extraits de plantes:Extraits (pharmacie)
Fibroblastes
Peau -- Soins et hygiène
StatistiqueIndex. décimale : 668.5 Parfums et cosmétiques Résumé : Skin ageing is an inevitable physiological process, determined by several concomitant factors, resulting in a thinner and drier skin, in the formation of fine wrinkles, and a gradual dermal atrophy.
During this process the skin cells become senescent, they slow down their metabolism and the production of structural matrix components. The Growth Differentiation Factor 11 (GDF11) has been recently identified as a pro-youth factor in several cell types, and in particular it was shown its involvement in the restoration of a young phenotype in skin fibroblasts, by stimulating important ExtraCellular Matrix proteins. Furthermore, its key role in modulating mitochondrial functions in skin cells during ageing has been highlighted. In the present article, we describe the characterisation of a plant extract, obtained from Rosa rugosatissue cultures, able to produce a ‘rejuvenating’ effect in aged skin fibroblasts by inducing GDF11 expression and synthesis. The extract was also capable of increasing the expression of PGC1alpha, the master regulator of the mitochondrial biogenesis, and other important genes involved in the pathway, leading to an improvement of cell metabolism and longevity.Note de contenu : - MATERIALS AND METHODS : Plant tissue cultures - Chemical analyses - Cell cultures and reagents for cell bioassays - ELISA assays - Gene expression analysis in HDF - Smad2 reporter assay - ATP content determination - Statistical analyses - Clinical tests
- RESULTS AND DISCUSSION : Preparation of the Rosa rugosa extracts and cytotoxicity assay - Activity of the Rosa rugosa extracts on the expression of GDF11, Sirtuins 1 and Sirtuin 6 - Chemical analysis of the Rosa rugosa somatic embryo extract- Activities of the Rosa rugosa somatic embryo extract on mitochondria - Activities of the Rosa rugosasomatic embryo extract on collagen and GDF11 production - Clinical tests with the Rosa rugosasomatic embryo extractEn ligne : https://drive.google.com/file/d/14RZdDOU35aptcxwYpk23FioS_DVj6Y6g/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33962
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Code-barres Cote Support Localisation Section Disponibilité 21621 - Périodique Bibliothèque principale Documentaires Disponible A new matrikine-derived peptide up-regulates longevity genes for improving extracellular matrix architecture and connections of dermal cell with its matrix / R. Leroux in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 42, N° 1 (02/2020)
[article]
Titre : A new matrikine-derived peptide up-regulates longevity genes for improving extracellular matrix architecture and connections of dermal cell with its matrix Type de document : texte imprimé Auteurs : R. Leroux, Auteur ; Caroline Ringenbach, Auteur ; Thibault Marchand, Auteur ; Olivier Peschard, Auteur ; Philippe Mondon, Auteur ; Pascaline Criton, Auteur Année de publication : 2020 Article en page(s) : p. 53-59 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cellules -- Cultures et milieux de culture
Composés organiques -- Synthèse
Dermatologie
Elisa, TestLe test ELISA est une méthode de laboratoire qualifiée d'immuno-enzymatique. Il s'agit d'un test immunologique, car il a pour principal objectif de mettre en évidence la présence d'anticorps ou d'antigènes spécifiques à une pathologie, dans un échantillon de sang. Cet examen utilise une protéine appelée enzyme, qui va se fixer à certains constituants spécifiques de la maladie, et par repérage et quantification de cette enzyme, la maladie pourra être affirmée, ainsi que son intensité évaluée.
Génétique
Peau -- Anatomie
Peau -- Physiologie
Peptides
ProtéomiqueLa protéomique désigne la science qui étudie les protéomes, c'est-à -dire l'ensemble des protéines d'une cellule, d'un organite, d'un tissu, d'un organe ou d'un organisme à un moment donné et sous des conditions données.
Dans la pratique, la protéomique s'attache à identifier de manière globale les protéines extraites d'une culture cellulaire, d'un tissu ou d'un fluide biologique, leur localisation dans les compartiments cellulaires, leurs éventuelles modifications post-traductionnelles ainsi que leur quantité.
Elle permet de quantifier les variations de leur taux d'expression en fonction du temps, de leur environnement, de leur état de développement, de leur état physiologique et pathologique, de l'espèce d'origine. Elle étudie aussi les interactions que les protéines ont avec d'autres protéines, avec l'ADN ou l'ARN, ou d'autres substances.
La protéomique fonctionnelle étudie les fonctions de chaque protéine.
La protéomique étudie enfin la structure primaire, secondaire et tertiaire des protéines. (Wikipedia)Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Skin extracellular matrix (ECM) is a dense and well-organized structure produced by fibroblasts. This ECM transduces environmental mechano-signals to cell nucleus through the integrin–actin complex, thus triggering ECM protein syntheses. The aim of this study was to discover a novel peptide, structurally related to dermal matrikines, that promotes syntheses of ECM components.
- Methods and results : Screening tests with 120 peptides were carried out by using normal dermal human fibroblasts (HF). As a result, one candidate of interest was isolated, the N-Prolyl Palmitoyl Tripeptide-56 Acetate (PP56), which increases collagen and fibronectin productions at gene and/or protein levels. Using liquid chromatography–tandem mass spectrometry (LC-MS/MS), a recent and innovative analytical technology, in addition to more traditional techniques, it was showed that two metabolic pathways were significantly modulated: one for collagen production and one for actin. Moreover, this peptide up-regulated the transcription of Forkhead Box O (FOXO) and sestrin messenger RNAs (mRNA), leading to production of proteins involved into longevity and more recently in collagen production.
- Results : Results indicated that this peptide is a potential candidate to improve ECM density and organization in a new way.DOI : 10.1111/ics.12584 Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33679
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Code-barres Cote Support Localisation Section Disponibilité 21486 - Périodique Bibliothèque principale Documentaires Disponible Niacinamide mitigates SASP-related inflammation induced by environmental stressors in human epidermal keratinocytes and skin / John C. Bierman in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 42, N° 5 (10/2021)
[article]
Titre : Niacinamide mitigates SASP-related inflammation induced by environmental stressors in human epidermal keratinocytes and skin Type de document : document électronique Auteurs : John C. Bierman, Auteur ; Timothy Laughlin, Auteur ; Makio Tamura, Auteur ; Ben C. Hulette, Auteur ; Catherine E. Mack, Auteur ; Joseph D. Sherrill, Auteur ; Christina Y. R. Tan, Auteur ; Malgorzata Morenc, Auteur ; Sophie Bellanger, Auteur ; John. E Oblong, Auteur Année de publication : 2020 Article en page(s) : p. 501-511 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Anti-inflammatoires
Cellules -- Cultures et milieux de culture
Justification de l'allégation
Nicotinamide
Peau -- Inflammation
Peau -- Physiologie
Stress -- Effets physiologiques
Stress environnementalIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : To evaluate whether niacinamide (Nam) can mitigate production of inflammatory and senescence-related biomarkers induced by environmental stressors.
- Methods : Human epidermal keratinocytes were exposed to UVB, urban dust, diesel exhaust and cigarette smoke extract and treated with Nam or vehicle control. Full thickness 3-D skin organotypic models were exposed to a combination of UVB and PM2.5 and treated with Nam or vehicle control. Quantitation of the SASP-related inflammatory mediators PGE2, IL-6 and IL-8 was performed on cultured media. UVB-exposed keratinocytes treated with and without Nam were immunostained for the senescence biomarker Lamin B1 (LmnB1). Transcriptomics profiling of cigarette smoke extract effects on keratinocytes was performed. A double-blind, placebo-controlled clinical was conducted on 40 female panellists that were pretreated on back sites for two weeks with 5% Nam or vehicle and then exposed to 1.5 minimal erythemal dose (MED) solar-simulated radiation (SSR). Treated sites were compared with non-treated exposed sites for erythema and the skin surface IL-1αRA/IL-1α inflammatory biomarkers.
- Results : Ultraviolet B induced synthesis of PGE2, IL-8 and IL-6 and reduced LmnB1 levels in keratinocytes. Urban dust and diesel exhaust only stimulated synthesis of IL-8 whereas cigarette smoke extract only stimulated levels of PGE2. In all exposures, treatment with Nam significantly mitigated synthesis of the inflammatory mediators and restored levels of UVB-reduced LmnB1. In the 3D skin equivalent model, Nam reduced IL-8 levels stimulated by a combination of topical PM2.5 and UV exposure. In a UV challenge clinical, pretreatment with 5% Nam reduced erythema and skin surface IL-1αRA/IL-1α inflammatory biomarkers that were induced by SSR.
- Conclusion : Since it is known that Nam has anti-inflammatory properties, we tested whether Nam can inhibit environmental stress-induced inflammation and senescence-associated secretory phenotype (SASP) biomarkers. We show Nam can reduce PGE2, IL-6 and IL-8 levels induced by environmental stressors. Additionally, in vivo pretreatment with Nam can reduce UV-induced erythema and skin surface inflammatory biomarkers. These findings add to the body of evidence that Nam can mitigate the skin’s inflammatory response elicited by environmental stressors. This supports Nam can potentially inhibit senescence and premature ageing and thereby maintain skin’s functionality and appearance.Note de contenu : - MATERIALS AND METHODS : Cells and reagents - ATP measurement - PGE2, IL-6 and IL-8 measurements - Lamin B1 and Ki-67 immunofluorescence staining - Eigene gene analysis - UV challenge study
- RESULTS : Nam mitigates UV-induced inflammatory response - Secretion of inflammatory signals and gene expression patterns induced by environmental stressors in keratinocytes is mitigated by Nam - Treatment with topical 5% Nam can reduce UV-induced erythema and the ratio of the skin surface inflammatory biomarkers IL-1 aRA/IL-1 aDOI : https://doi.org/10.1111/ics.12651 En ligne : https://drive.google.com/file/d/1JMyefjRlUnyZowe-9UsgBOlh5jEiZyVL/view?usp=shari [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35415
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 42, N° 5 (10/2021) . - p. 501-511[article]A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology / Jessica Welzel in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 44, N° 2 (04/2022)
[article]
Titre : A novel cell line from human eccrine sweat gland duct cells for investigating sweating physiology Type de document : document électronique Auteurs : Jessica Welzel, Auteur ; Sabine Grüdl, Auteur ; Bernhard Banowski, Auteur ; Holger Stark, Auteur ; Andrea Sättler, Auteur ; Thomas Welss, Auteur Année de publication : 2022 Article en page(s) : p. 216-231 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Analyse génétique
Cellules -- Cultures et milieux de culture
Expression génique
Glandes sudoriparesLes glandes sudoripares (sudorales) sont des annexes cutanées (organes microscopiques spécialisés) qui sécrètent la sueur et, pour certaines d'entre elles, des hormones ou phéromones.
Histologiquement, il s'agit de glandes exocrines (épithéliums cubiques bistratifiés) dont la fonction première est la transpiration.
Les deux types de glandes sudoripares chez l'humain
Chez l'humain, on distingue deux sortes de glandes sudoripares qui diffèrent par leur origine embryologique, leurs fonctions, leurs répartitions et par la composition de la sueur qu'elles excrètent.
Glandes sudoripares « eccrines »
Les glandes sudoripares eccrines sont de loin les plus nombreuses, de trois à six millions, avec une densité moyenne de 200 glandes/cm2. Leur topographie est quasi-ubiquitaire, avec une répartition sur presque l'ensemble de la surface cutanée, avec une plus forte densité au niveau de la paume des mains, de la plante des pieds (où elles atteignent une densité maximale de 600 glandes/cm2) et sur le front.
Ces glandes sont absentes au niveau des petites lèvres et du clitoris chez les femmes, et du gland chez l'homme.
Glandes sudoripares « apocrines »
Chez l'humain, ces glandes se trouvent sous les aisselles (dans l'organe axillaire), sur la peau autour de l'anus et autour des mamelons. À la différence des glandes eccrines, la répartition des glandes apocrines est donc plus restreinte. Par ailleurs, les glandes apocrines présentent une plus grande taille, leur canal excréteur s'abouche dans un follicule pileux par lequel leur sécrétion est déversée ; celle-ci contient des lipides et des phéromones, des composés transformés par le microbiote bactérien cutané, produisant l'odeur de transpiration.
Peau -- Histologie
VirusIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Human eccrine sweat glands (eSG) represent vital components of the skin involved in regulating body temperature. Especially the eccrine duct, which opens directly into the skin surface and releases the aqueous sweat, constitutes the first contact point with topically applied substances. For scientific investigations and to understand the underlying sweating mechanism on a cellular level defined cellular material is beneficial. We, therefore, strived to generate a cell line derived from human eSG duct cells for identifying new mechanisms in sweating control, as such a standardized cell line is currently lacking.
- Material and methods : Isolated primary human eSG duct cells were transduced with simian virus 40 large T antigen (SV40T) by lentiviral transduction. Successfully SV40T-transduced clones were selected by single-cell cloning with one clone, named 1D10, being particularly described in this work.
- Results : In performed cellular investigations, SV40T-transduced duct-derived cells exhibited an extended lifespan with stable population doubling times suggesting its immortality. Besides, 1D10 clonal cell culture demonstrated similarities with parental, primary duct cells regarding gene expression of selected sweat gland-related markers. When combined with primary coil cells in a hanging drop co-culture, those transduced duct-derived cells showed some duct cell-like features. Further, a certain degree of cellular communication and a specific reaction towards substance application was observed.
- Conclusion : Generated and herein described cell line derived from isolated human eSG duct cells is, based on the presented scientific findings, considered as immortal. Besides, this cell line shows some similarity with primary duct cells, although alterations from native glands were detected, among which is loss of expression of cystic fibrosis transmembrane conductance regulator. Provided some further investigations, presented SV40T-transduced duct-cell derived cell line seems a suited surrogate of primary eccrine duct cells.Note de contenu : - METHODS : Cell culture - Gene expression analysis - Histological examinations
- RESULTS : Characteristics of SV40T-transduced duct-derived cells -
Characteristics of clone 1D10
- DISCUSSION : Establishment of an immortalized eccrine sweat gland duct cell line - Characterization of SV40T-transduced eccrine sweat gland duct-derived cell clone 1D10DOI : https://doi.org/10.1111/ics.12769 En ligne : https://drive.google.com/file/d/1LYIf5UT6xVWsFaaYEX2sxn2Anyn2OWhc/view?usp=shari [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37724
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 2 (04/2022) . - p. 216-231[article]Objectiver le whitening in ADDIACTIVE, N° 83 (04-05-06/2012)
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