Melanogenesis-promoting effect of Cirsium japonicum flower extract in vitro and ex vivo / Minkyung Kim in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 43, N° 6 (12/2021)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 6 (12/2021) Titre : Melanogenesis-promoting effect of Cirsium japonicum flower extract in vitro and ex vivo Type de document : document électronique Auteurs : Minkyung Kim, Auteur ; Kyungeun Jeon, Auteur ; Seoungwoo Shin, Auteur ; Sohyun Yoon, Auteur ; Hayeon Kim, Auteur ; Hee Young Kang, Auteur ; Dehun Ryu, Auteur ; Deokhoon Park, Auteur ; Eunsun Jung, Auteur Année de publication : 2021 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Adénosine monophosphate cyclique L'adénosine monophosphate cyclique (ou AMP cyclique ou AMPc) est une molécule biologique qui agit souvent en tant qu'intermédiaire, dans l'action des hormones ou des neurotransmetteurs notamment. Elle fait partie des seconds messagers. Cosmétiques Extraits de plantes:Extraits (pharmacie) Follicule pileux Mélanogénèse Peau -- Soins et hygiène Peau reconstituée Pigmentation de la peau Pigmentation, Troubles de la Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : In this study, we examined the effect of C. japonicum flower extract (CFE) on melanogenesis and its mechanism in vitro and ex vivo. - Methods : The effect of CFE on melanogenesis was investigated with lightly (HEMn-LP) and moderately (HEMn-MP) pigmented normal human melanocytes, reconstituted three-dimensional skin (3D skin) model and ex vivo human hair follicles. The melanogenesis-inducing effect of CFE was evaluated using melanin content and intracellular tyrosinase activity assay. The amount and type of eumelanin and pheomelanin were analysed by using HPLC method. The mechanism involved in the effect of CFE on hyperpigmentation was explored by cyclic adenosine monophosphate (cAMP) immunoassay and western blot analysis for tyrosinase, microphthalmia-associated transcription factor (MITF) and phosphorylated CRE-binding protein (pCREB) expression. The degree of pigmentation in 3D skin and L-values were measured using a CR-300 chroma meter. The amount of dissolved melanin was measured using a spectrophotometer. The content of melanin in the hair follicles was evaluated by Fontana Masson staining. - Results : C. japonicum flower extract significantly increased the melanin content and cellular tyrosinase activity in both HEMn-LP and HEMn-MP cells. The markers of pheomelanin and eumelanin in HEMn-LP and HEMn-MP were also increased by CFE. We observed that CFE treatment on melanocytes increased intracellular cAMP with inducing pCREB and up-regulating the protein levels of TYR and MITF. Furthermore, CFE considerably increased the melanin content in a 3D skin model and ex vivo human hair follicles. - Conclusions : These results suggest that CFE exerts hyperpigmentation activity through cAMP signalling in human melanocytes that it can improve follicular depigmentation and vitiligo by stimulating the melanin synthesis. Note de contenu : - MATERIALS AND METHODS : Materials - Cell culture and viability assay - Melanin content and cell viability assay - Analysis of eumelanin and pheomelanin by HPLC - Cellular tyrosinase activity - Cyclic AMP immunoassay - Western blot analysis - The reconstituted three-dimensional human skin model - Ex vivo human hair follicles - Statistical analysis - RESULTS : Effect of CFE on the melanin content of lightly and moderately pigmented human melanocytes - Analysis on markers of pheomelanin and eumelanin - Effect of CFE on cellular tyrosinase activity - Effect of CFE on cAMP signalling pathway - Melanogenic effect of CFE on 3D skin model - Table 1 : TTCA and PTCA as specific markers in lightly and moderately pigmented melanocytes DOI : https://doi.org/10.1111/ics.12746 En ligne : https://drive.google.com/file/d/1ujuVw-VGc03qkR-B5zlcoeYHm0Vn4ygI/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37051 [article]

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
aucun exemplaire

The effect of Salix alba L. bark extract on dark circles in vitro and in vivo / Suwon Jeon in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 5 (10/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 5 (10/2023) . - p. 636-646 Titre : The effect of Salix alba L. bark extract on dark circles in vitro and in vivo Type de document : texte imprimé Auteurs : Suwon Jeon, Auteur ; Sohyun Yoon, Auteur ; Yuna Kim, Auteur ; Seoungwoo Shin, Auteur ; Hyanggi Ji, Auteur ; Eunae Cho, Auteur ; Deokhoon Park, Auteur ; Eunsun Jung, Auteur Année de publication : 2023 Article en page(s) : p. 636-646 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Barrière cutanée Cellules -- Cultures et milieux de culture Cernes Cosmétiques Dermo-cosmétologie Extraits de plantes:Extraits (pharmacie) Formulation (Génie chimique) Ongle -- Anatomie Peau -- Physiologie Peau -- Soins et hygiène Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Dark circles in the infraorbital area are a common cosmetic concern among individuals because they exhibit fatigue and are undesirable across all ages. Of the dark circle etiologies, blood stasis by poor-vascular integrity can cause darkening of the lower eyelid skin, which might be alleviated by reduced endothelial permeability. In this study, we investigated the effects of Salix alba bark extract (SABE) on the synthesis of hyaluronic acid (HA) in fibroblasts and vascular integrity protection from inflammatory cytokine. We also performed a clinical trial investigating the effect of SABE on dark circles. - Methods : To confirm the effect of SABE on HA synthesis in human dermal fibroblasts (HDFs), we performed ELISA and real-time PCR. We investigated the interaction HDF-secreted substance with vascular integrity, and human dermal microvascular endothelial cells (HMEC-1) were treated with conditioned medium (CM) from HDF treated with or without SABE. Subsequently, we conducted a clinical study on 29 subjects by having them apply SABE containing cream for 8 weeks. - Results : Salix alba bark extract treatment increased HA synthesis and regulated HMW–HA-related gene expressions in HDF. CM from SABE-treated HDF alleviated endothelial permeability and led to improved vascular integrity in HMEC-1 cells. Treatment with the cream containing 2% SABE for 8 weeks improved the parameters measuring dark circles, skin microcirculation and elasticity. - Conclusion : Our results showed that SABE could protect against dark circles in vitro, and that topical treatment of SABE improved the clinical indexes of dark circles in a clinical study. Therefore, SABE can be used as an active ingredient for improving dark circles. Note de contenu : - MATERIALS AND METHODS : Preparation of Salix alba bark extract (SABE) - Cell culture and reagent - Hyaluronic acid enzyme-linked immunosorbent assay (ELISA) - RNA preparation and quantitative realtime PCR - Endothelial permeability assays - Preparation of cream - Clinical trial on dark circles - RESULTS : SABE increases HA synthesis and regulates HA-related genes in HDF - Conditioned medium produced by HDF reinforces vascular endothelial integrity in HMEC-1 - Effect of SABE on infraorbital dark circles in clinical trial - Table 1 : Formulation of test and placebo cream - Table 2 : Dark circles grade scale Référence de l'article : https://doi.org/10.1111/ics.12873 En ligne : https://drive.google.com/file/d/1obc6x3Pt5lk3aseRPepM4h_TCBrfAukX/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40090 [article]

Exemplaires

Code-barres	Cote	Support	Localisation	Section	Disponibilité
aucun exemplaire

---

1 (1 - 2 / 2)