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A human skin model to evaluate the protective effect of compounds against UVA damage / Sophie Abadie in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 41, N° 6 (12/2019)
[article]
Titre : A human skin model to evaluate the protective effect of compounds against UVA damage Type de document : texte imprimé Auteurs : Sophie Abadie, Auteur ; Philippe Bedos, Auteur ; J. Rouquette, Auteur Année de publication : 2019 Article en page(s) : p. 594-603 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Barrière cutanée
Dermatologie
Explant de peau
Peau -- Anatomie
Peau -- Physiologie
Rayonnement ultraviolet -- Effets physiologiques
Rayonnement ultraviolet A
Tests de sécuritéIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : The main function of skin is to protect the body from external aggressions. Over time, normal skin ageing is accelerated by external stresses such as smoking, pollution, chemical products and radiation. UV light, in particular UVA, causes DNA damage, apoptosis and morphological modifications, which are responsible for both premature ageing and cancer. The aim of this study was to establish a discriminatory and sufficiently reproducible cutaneous model for evaluating UVA damage, to enable testing for effectiveness of potentially protective compounds.
- Methods : The cutaneous model is based on Human skin explants irradiated with UVA. Deleterious effects on epidermis were observed and quantified by haematoxylin–eosin staining and by immunofluorescence of y-H2Ax, cytokeratin 5, involucrin and loricrin protein. Dermis deterioration was evaluated by transmission electronic microscopy and zymography in situ.
- Results : We were able to observe and quantify deleterious effects associated with UVA irradiation: epidermal and dermal disruption, appearance of Sunburn cells, increased DNA damage and induced apoptosis. The use of this model in the evaluation of protective compounds was first confirmed using sunscreens, then further validated with a panel of active ingredients which showed beneficial effects on epidermis morphology and DNA integrity after UVA exposure.
- Conclusion : We have developed a model and a standardized protocol, based on the use of human skin explants, which allows us to explore the protective effect of active ingredients to environmental stresses such as UVA.Note de contenu : - MATERIALS AND METHODS : Human skin tissue collection - UVA irradiation - Compounds - Histological analysis - Immunostaining - TUNEL assay - Transmission electron microscopy on dermis - Zymography in situ - Statistical analysis
- RESULTS : UVA irradiation causes epidermal disruption - UVA irradiation induces double-strand DNA breaks and apoptosis of keratinocytes - Effects of UVA irradiation on the extracellular matrix of the dermis - A cutaneous model to explore the effect of new protective compounds against UVADOI : 10.1111/ics.12579 Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33668
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Code-barres Cote Support Localisation Section Disponibilité 21409 - Périodique Bibliothèque principale Documentaires Disponible Schisandra chinensis combats pollution-induced stress / Carine Boutot in PERSONAL CARE EUROPE, Vol. 12, N° 5 (11/2018)
[article]
Titre : Schisandra chinensis combats pollution-induced stress Type de document : texte imprimé Auteurs : Carine Boutot, Auteur ; Edwige Ranouille, Auteur ; Emilie Bony, Auteur ; Jean-Yves Berthon, Auteur ; Edith Filaire, Auteur ; Claire Leduc, Auteur ; Philippe Bedos, Auteur Année de publication : 2018 Article en page(s) : p. 29-32 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Actifs anti-pollution
Biomolécules actives
Etudes cliniques
Extraits de plantes:Extraits (pharmacie)
KératinocytesLes kératinocytes sont des cellules constituant 90 % de la couche superficielle de la peau (épiderme) et des phanères (ongles, cheveux, poils, plumes, écailles). Ils synthétisent la kératine (kératinisation), une protéine fibreuse et insoluble dans l'eau, qui assure à la peau sa propriété d'imperméabilité et de protection extérieure.
L'épiderme est divisé en 4 couches basées sur la morphologie des kératinocytes (de l'intérieur vers l'extérieur) :
1. stratum germinativum (couche basale à la jonction avec le derme)
2. stratum spinosum
3. stratum granulosum
4. stratum lucidum
5. stratum corneum
Les kératinocytes passent progressivement de la couche basale vers les couches supérieures par différenciation cellulaire jusqu'au stratum corneum ou ils forment une couche de cellules mortes nommées squames, par apoptose. Cette couche constitue une barrière de protection et réduit la perte d'eau de l'organisme.
Les kératinocytes sont en perpétuel renouvellement. Ils mettent environ 1 mois pour aller de la couche basale au stratum corneum mais ce processus peut être accéléré en cas d'hyperprolifération de kératinocyte (psoriasis).
Peau -- Soins et hygiène
StatistiqueIndex. décimale : 668.5 Parfums et cosmétiques Résumé : The human skin, and mainly the upper layer of the epidermis, plays the role of a barrier, but is also one of the first and major targets of air pollutants, pollutants contributing to wrinkle and dark sports occurrence through the redox imbalance. A possible approach to attack ROS-mediated disorders for both preventive and treatment means is based on the use of substances, which can be found in plants as secondary metabolites, lignans being a promise candidate. The present study was aimed to better understand the cellular mechanisms beyond the oxidative changes induced by urban pollution (Urban dust 1649b, NIST) and the effect of Schisandra chinensis (S. chinensis) extract in reconstructed human epidermis, by a transcriptomic approach and secondly through the evaluation of Nrf2, AhR, NF-kB, and DJ-1 pathways using an in vitro model. Finally, we evaluated the effect of S. chinensis on skin hydration, homogeneity, radiance and luminosity in Chengdu (China). Urban dust (80pg.ml") was able to activate the cytoplasmic expression of NF-kB and AhR when compared to control. S.chinensis extract attenuated the urban dust-induced oxidative stress, the protective mechanism being associated, at least in part, with the modulation of the Nrf2 and AhR pathways and the activation of DJ-1. S. chinensis extract, named Urbalys protects from prolonged pollution aggression since it improves hydration, protects skin homogeneity, increases skin radiance and attenuates skin spot intensity after 21 days of pollution exposition. Note de contenu : - TRANSCRIPTOMIC ANALYSES : Effect of extract from S.chinensis on the protection of human keratinocytes (NHEK) damages caused by pollution - Clinical study - Statistical analysis
- RESULTS : Transcriptomic results - Effeect of urban dust and co-treatment on NF-kB activation and cellular pathways in human keratinocytesEn ligne : https://drive.google.com/file/d/1OIpD3pz2TDSTFo04vMQTYrDufs9xLRh9/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=31276
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Code-barres Cote Support Localisation Section Disponibilité 20328 - Périodique Bibliothèque principale Documentaires Disponible Stimulating Nrf2 and inhibiting NF-kB to help skin combating pollution / E. Bony in SOFW JOURNAL, Vol. 144, N° 3 (03/2018)
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Titre : Stimulating Nrf2 and inhibiting NF-kB to help skin combating pollution Type de document : texte imprimé Auteurs : E. Bony, Auteur ; I. Renimel-Krolkiewicz, Auteur ; Jean-Yves Berthon, Auteur ; C. Leduc, Auteur ; Philippe Bedos, Auteur ; P. Schlegel, Auteur Année de publication : 2018 Article en page(s) : p. 16-22 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Actifs anti-pollution
Cosmétiques
Peau -- Effets de la pollution atmosphérique
Peau -- Soins et hygièneIndex. décimale : 668.5 Parfums et cosmétiques Résumé : The skin is routinely exposed to stresses from environmental pollution. These repeated and regular exposures can profoundly affect skin physiology, and may lead to irreversibie consequences. We studied the potential of a Schisandra chinensis (S.C.) extract, particularly ennched in lignans, to enhance skin defense mechanisms involved in pollution stress response, with the main pathways involved in skin being Nuclear Factor Kappa B (NF-kB), DJ-1, the nuclear factor erythroid 2-related factor 2 (Nrf2) and aryl hydrocarbon receptor (AhR) signaling pathways. Using an in vitro model, we showed that co-treatment with Urban dust and S.C. extract was able to stimulate the expression of Nrf2 and DJ-1, as well as the decrease of NF-kB and AhR, suggesting that this active extract may provide wide protection for skin against daily environmental insults. In a skin equivalent model stressed by Urban dust, the extract provided a global protection of skin components as shown by key proteins involved in the epidermal barrier. Even if further investigations are needed, we speculate that S.C. extract can be used to butter the harmful effects.of free radicals and enhance the skin barrier,due to air pollutant interactions with the skin, allowing a global preservation of cutaneous structures. Note de contenu : - Fig. 1. Summarizes the molecular mechanisms for pollutant-induced ROS generation and pro-inflammatory cytokines such as IL-1α, IL-8, and TNF-α, resulting i increased skin aging. Mitogen-activated protein kinases (MAPK) ; NF-KB : nuclear factor KB ; AP-A : activator protein-1 ; PI3K : phosphoinositide 3-kinase
- Fig. 2. Polycyclic aromatic hydrocarbons (PAHs) and particulate matter (PM) induce ROS generation and pro-inflammatory cytokines. Upon ligation by PAHs and PM, the activated AhR translocates form the cytoplasm into the nucleus. This translocated AhR binds with ANRT, resulting in the activation of cytochrome P450, family 1, member A1 (CYP1A1) transcription. ROS generated by CYP1A1 stimulates the production of TNF-α and IL-8
- Fig. 3. NK-KB cytoplasm expression after Urban dust exposure or co-treatment with Urban dust and S.C. extract ***p<0.001 versus Urban dust
- Fig. 4. AhR cytoplasm expression after Urban dust exposure or co-treatment with Urban dust and S.C. extract ***p<0.001 versus Urban dust
- Fig. 5. NrF2 cytoplasm expression after Urban dust exposure or co-treatment with Urbandust and S.C. extrct
- Fig. 6. DJ-1 expression after Urban dust exposure or co-treatment with Urban dust and S.C. extract ***p<0.001 versus Urban dust
- Fig. 7. Secretion of PGE2 and il-6 in polluted or non-reconstructed skin supernatant treated or not with S.C. extract. Analysis has been done 24h after pollutant stress. ***p<0.001 versus Urban dust - 8. MDA expression in epidermis of skin equivalent model. Analysis has been done 24h after pollutant stressEn ligne : https://drive.google.com/file/d/1YZRvsU-82xTpVPt-JfmooGJlU2VaVl8w/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=30351
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Code-barres Cote Support Localisation Section Disponibilité 19758 - Périodique Bibliothèque principale Documentaires Disponible The alkaloid centcyamine increases expression of klotho and lamin B1, slowing the onset of skin ageing in vitro and in vivo / Sophie Abadie in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 43, N° 5 (10/2021)
[article]
Titre : The alkaloid centcyamine increases expression of klotho and lamin B1, slowing the onset of skin ageing in vitro and in vivo Type de document : document électronique Auteurs : Sophie Abadie, Auteur ; Claire Leduc, Auteur ; Karl Lintner, Auteur ; Philippe Bedos, Auteur Année de publication : 2021 Article en page(s) : p. 561-572 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Centcyamine
Génétique
Justification de l'allégation
Peau -- Physiologie
ProtéomiqueLa protéomique désigne la science qui étudie les protéomes, c'est-à -dire l'ensemble des protéines d'une cellule, d'un organite, d'un tissu, d'un organe ou d'un organisme à un moment donné et sous des conditions données.
Dans la pratique, la protéomique s'attache à identifier de manière globale les protéines extraites d'une culture cellulaire, d'un tissu ou d'un fluide biologique, leur localisation dans les compartiments cellulaires, leurs éventuelles modifications post-traductionnelles ainsi que leur quantité.
Elle permet de quantifier les variations de leur taux d'expression en fonction du temps, de leur environnement, de leur état de développement, de leur état physiologique et pathologique, de l'espèce d'origine. Elle étudie aussi les interactions que les protéines ont avec d'autres protéines, avec l'ADN ou l'ARN, ou d'autres substances.
La protéomique fonctionnelle étudie les fonctions de chaque protéine.
La protéomique étudie enfin la structure primaire, secondaire et tertiaire des protéines. (Wikipedia)
VieillissementIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Background : Klotho is a protein known for its beneficial effects on longevity. Centcyamine is an alkaloid present in certain plants whose extracts have an anti-inflammatory effect. Skin fibroblasts are essential to the formation and structure of the dermis.
- Objective : Centcyamine is an indole-based alkaloid composed of coumaric acid, a resveratrol precursor and methoxytryptamine, which can be both a precursor, or a derivative, of melatonin. Given these building blocks and their well-known bioactivities, it was of interest to explore the potential benefits of using this aryl-alkaloid, in cosmetic skincare applications.
- Methods : We tested cultured normal human dermal fibroblasts (NHDF) in vitro to observe how supplementation with centcyamine improves properties of the cells to counteract the effect of ageing. The expression of genes and proteins of interest was quantified. The effect on doubling time and cell function was evaluated following treatment of the cells over several replication cycles. Skin firmness, red spot index and skin isotropy were measured with Dynaskin®, Visia® and Primos® equipment, respectively, and compared over two months in a vehicle-controlled clinical trial on 60 persons.
- Results : Centcyamine activates the expression of the gene KL and the related protein Klotho in dermal fibroblasts. Moreover, centcyamine slows the replicative ageing process of fibroblasts in culture. These cells retain cellular functions identical to those of young cells: the synthesis of lamin B1, a crucial regulatory protein of proliferation, as well as of collagen I and elastin is retained in aged cells. The clinical data are shown to improve skin isotropy in a majority of subjects, to reduce the red spot intensity and to maintain skin firmness in the treated group vs. the vehicle.
- Conclusion : The alkaloid centcyamine induces changes in the metabolism of the ageing process of human dermal fibroblasts. The up-to-now unobserved implication of both Klotho and lamin B1 to maintain homeostasis of the skin opens new venues for the prevention of age-related changes in skin structure. The in vitro and clinical data, while not demonstrated to be causally related, converge towards a common goal of skin repair and slower ageing processes.Note de contenu : - MATERIAL AND METHODS : Effect of centcyamine on fibroblasts - Effect of centcyamine on replicative ageing of fibroblasts - Clinical trial - Statistical analysis
- RESULTS : Gene array - Protein synthesis - Cell proliferation and function - Clinical trial
- Table 1 : Centcyamine modulates gene expression in dermal fibroblast : qPCR on NHDF after 24h of Centcyamine treatmentDOI : https://doi.org/10.1111/ics.12731 En ligne : https://drive.google.com/file/d/1DV4r7r8Kf2HRANTGmjL83vBpp5JhohVc/view?usp=shari [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36860
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 5 (10/2021) . - p. 561-572[article]