Antibiotic susceptibility patterns of gram-negative moderately halophilic bacteria isolated from salted skins / Pinar Caglayan in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 104, N° 2 (03-04/2020)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 104, N° 2 (03-04/2020) . - p. 67-76 Titre : Antibiotic susceptibility patterns of gram-negative moderately halophilic bacteria isolated from salted skins Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Antonio Ventosa, Auteur Année de publication : 2020 Article en page(s) : p. 67-76 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antimicrobiens Bactéries à gram négatif Cuirs et peaux de chèvres Résistances aux antibiotiques Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Antimicrobial activities of 16 different antibiotics against 47 Gram-negative bacteria isolated from salted skin samples were examined by Kirby-Bauer disc diffusion method. In the present study 10 strains isolated from 6 salted goatskin samples belonging to Russia, South Africa, Bulgaria, Australia, and 37 strains isolated from 14 salted sheepskin samples belonging to Greece, Australia, Bulgaria, South Africa, Israel, USA, Turkey were used as test strains. Susceptibilities of Halomonas venusta, Halomonas zhanjiangensis, Chromohalobacter beijerinckii, Halomonas alkaliphila, Halomonas eurihalina, Chromohalobacter israelensis, Halomonas halodenitrificans, Halomonas halmophila, Chromohalobacter canadensis, Chromohalobacter japonicus, ldiomarina loihiensis against amikacin, gentamicin, tobramycin, cefadroxil were not detected. In addition, susceptibilities of Halomonas eurihalina, Chromohalobacter israelensis and Chromohalobacter beijerinckii against sulfamethoxazole-trimethoprim and ampicillin ; aztreonam and ampicillin ; ampicillin-sulbactann and ampicillin were not observed, respectively. Although inhibition zones of Halomonas halodenitrificans (15-20mm), Halomonas halmophila (10-15mm), Chromohalobacterjaponicus (22-23mm), Idiomarina loihiensis (15mm) against ampicillin were detected, the other strains did not show any inhibition zones. All test strains were susceptible to the other antibiotics such as chloramphenicol, meropenem, imipenem, cefoxitin, cefuroxime, ceftriaxone, amoxycillin/clavulanic acid and piperacillin/tazobactam. All strains except Halomonas eurihalina were found to be susceptible to sulfamethoxazole-trimethoprim. The experimental results of this study demonstrated that 38%, 36%, 26% of the Gram-negative bacterial species isolated from salted sheep and goat skins were resistant against 31%, 25%, 38% of the antibiotics tested. Therefore, effective antibacterial applications should be applied in curing salt to kill multidrug resistant moderately halophilic Gram-negative bacterial species in leather industry. Note de contenu : - Moderately halophilic bacterial isolates - Examination of susceptibilities of the isolates against antibiotics using Kirby-Bauer disc diffusion method - Table 1 : Inhibition zone diameters (mm) of gram-negative moderately halophilic bacteria against different antibiotics - Table 2 : Multidrug resistance profiles of gram negative moderately halophilic bacteria isolated from salted skin samples En ligne : https://drive.google.com/file/d/1klssRAKDJ8jaSSzSS5OqEWs0c14hNXSL/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33911 [article]

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Characterization of moderately halophilic bacteria from salt-pack-cured hides / Pinar Caglayan in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 99, N° 5 (09-10/2015)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 99, N° 5 (09-10/2015) . - p. 250-254 Titre : Characterization of moderately halophilic bacteria from salt-pack-cured hides Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Cristina Sánchez-Porro, Auteur ; Antonio Ventosa, Auteur ; Meral Birbir, Auteur Année de publication : 2015 Article en page(s) : p. 250-254 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bactéries -- Identification Bactéries -- Inactivation Caractérisation Cuirs et peaux -- Conservation Peaux brutes -- Salage Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : In the present study, all hide sample contained moderately halophilic bacteria and 13 different species which belong to the genera of Salimicrobium, Halomonas, Alkalibacillus, Marinococcus, Oceanobacillus, Thalassobacillus, Chromohalobacter were isolated and identified with phenotypic analyses and molecular methods. It was found that moderately halophilic isolates perform biochemical processes to breakdown macromolecules such as protein, DNA, fats which are responsible for hides deterioration. Therefore, we suggest steps should be taken to examine and inactivate moderately halophilic bacteria in preservation salt to obtain high quality leather. Note de contenu : - Sample collection and growth conditions - Determination of total number of moderately halophilic bacteria - Biochemical tests of the isolates - Amplification and sequencinf of 16S rRNA genes - Nucleotides accession number En ligne : https://drive.google.com/file/d/1Y8FLn69ezJ_y6EhLoR8iaySh7HFtQ9M7/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24656 [article]

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Determination of minimum inhibitory concentrations of sophorolipids against selected halotolerant and moderately halophilic bacteria, and extremely halophilic archaea isolated from salt samples and salted skins / R. D. Ashby in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIX, N° 8 (08/2024)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIX, N° 8 (08/2024) . - p. 339-348 Titre : Determination of minimum inhibitory concentrations of sophorolipids against selected halotolerant and moderately halophilic bacteria, and extremely halophilic archaea isolated from salt samples and salted skins Type de document : texte imprimé Auteurs : R. D. Ashby, Auteur ; Meral Birbir, Auteur ; P. Yilmaz, Auteur ; J. Msanne, Auteur ; Antonio Ventosa, Auteur ; E. Yilmaz, Auteur Année de publication : 2024 Article en page(s) : p. 339-348 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Antimicrobiens Archées Les archées ou Archaea, "originel, primitif", anciennement appelés archéobactéries, sont des microorganismes unicellulaires procaryotes, c'est-à-dire des êtres vivants constitués d'une cellule unique qui ne comprend ni noyau ni organites, à l'instar des bactéries. D'apparence souvent semblable à ces dernières, les archées ont longtemps été considérées comme des bactéries extrêmophiles particulières, jusqu'à ce que les recherches phylogénétiques sur les procaryotes, commencées en 1965, aboutissent, avec les travaux de Carl Woese et George E. Fox, à la publication en 1977 d'un arbre phylogénétique fondé sur les séquences des gènes d'ARN ribosomique des organismes étudiés, arbre dans lequel les procaryotes étaient scindés en deux domaines distincts, celui des bactéries et celui des archées. (Wikipedia) Bactéries Concentration minimale inhibitrice (antimicrobiens) En microbiologie, la concentration minimale inhibitrice (CMI) est la plus faible concentration d'un produit chimique, généralement un médicament, qui empêche la croissance visible d'une ou de plusieurs bactéries. La CMI dépend du micro-organisme considéré, de l'être humain affecté (in vivo uniquement) et de l'antibiotique lui-même. La CMI est déterminée en préparant des solutions du produit chimique in vitro à diverses concentrations croissantes, en incubant les solutions avec des groupes séparés de bactéries en culture et en mesurant les résultats en utilisant une méthode de dilution standardisée (agar ou microdilution). Les résultats se classent ensuite comme "sensible", "intermédiaire" ou "résistant" à un antimicrobien particulier en utilisant un point d'arrêt. Les points d'arrêt sont des valeurs convenues, publiées dans les directives d'un organisme de référence, comme le US Clinical and Laboratory Standards Institute (CLSI), la British Society for Antimicrobial Chemotherapy (BSAC) ou le Comité européen sur les tests de sensibilité aux antimicrobiens (EUCAST). On a pu constater des écarts importants au niveau des points d'arrêt de divers pays européens au fil des ans, et entre ceux de l'EUCAST et du CLSI. Alors que la CMI est la concentration la plus faible d'un agent antibactérien nécessaire pour inhiber la croissance visible, la concentration bactéricide minimale (CBM) est la concentration minimale d'un agent antibactérien qui entraîne la mort bactérienne. Plus la CMI est proche de la CBM, plus le composé est bactéricide. La première étape de la découverte d'un médicament est souvent le dépistage d'un médicament candidat de banque de données pour les CMI contre les bactéries d'intérêt. En tant que tels, les CMI sont généralement le point de départ pour de plus grandes évaluations précliniques de nouveaux agents antimicrobiens. Le but de la mesure de la concentration minimale inhibitrice est de s'assurer que les antibiotiques sont choisis efficacement pour augmenter le succès du traitement. (Wikipedia) Cuirs et peaux -- Conservation Peaux salées Sels de sodium Sophorolipides Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Sophorolipids (SLs) are fungal/yeast glycolipids that possess antimicrobial activity. In this study, three different SL mixtures were produced by microbial fermentation from oleic acid (SL-o), palmitic acid (SL-p), and stearic acid (SL-s), and tested for their antimicrobial activity against various halotolerant and moderately halophilic bacteria, and against extremely halophilic archaea that were isolated from salt and salted skin samples, as well as bacterial and extremely halophilic archaeal reference strains. The minimum inhibitory concentrations (MICs) of each of the SLs against known halotolerant and halophilic microorganisms were determined by the broth microdilution test method using resazurin dye. The extremely halophilic archaeal strains were most susceptible to the antimicrobial action of the tested SLs with MIC values as low as 3.42 μg/mL. Conversely, the endospore-forming halotolerant test strains, although susceptible to SLs, showed MIC values as high as 109.38 μg/mL. The SL-o, SL-p, and SL-s each exhibited broad-spectrum activity, demonstrating growth-inhibition against ten salt-associated bacterial strains and three archaeal strains possessing proteolytic activity, lipolytic activity, or both properties. In addition, each of the SLs showed growth-inhibition against three bacterial reference strains and two extremely halophilic archaeal reference strains. The findings from this study suggest that SL-o, SL-p, and SL-s might be utilized as potential antimicrobial agents together with salt, contributing to the preservation of the leather industry’s salted skins and hides. Note de contenu : - Materials - Production, isolation, purification, and analysis of sophorolipids - Inoculation procedure for test microorganisms - Preparation of resazurin solution - Determination of MICs of sophorolipid solutions and ethanol against test strains - Table 1 : Diacetylated lactone distribution for the sophorolipids used in this study produced from palmitic acid (SL-p), stearic acid (SL-s), and oleic acid (SL-o) according to their hydroxy fatty acid content - Table 2 : MIC values of SL-o, SL-p, SL-s (μg/mL) and ethanol (%) against halotolerant bacterial strains, moderately halophilic bacterial strains, bacterial reference strains, extremely halophilic archaeal strains, and extremely halophilic archaeal reference strains DOI : https://doi.org/10.34314/jwv5ae27 En ligne : https://drive.google.com/file/d/1k433IxtoIH-El2k7ndk5zFzUu1IH5J7w/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=41287 [article]

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Investigation of moderately halophilic bacteria causing deterioration of the salted sheep and goat skins and their extermination via electric current applications / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIII, N° 2 (02/2018)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIII, N° 2 (02/2018) . - p. 41-52 Titre : Investigation of moderately halophilic bacteria causing deterioration of the salted sheep and goat skins and their extermination via electric current applications Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Cristina Sánchez-Porro ; Antonio Ventosa ; Yasar Birbir Année de publication : 2018 Article en page(s) : p. 41-52 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries Courants alternatifs Courants électriques Cuirs et peaux -- Analyse Cuirs et peaux -- Conservation Cuirs et peaux de chèvres Cuirs et peaux de moutons Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Determination of moderately halophilic bacterial counts on the salted skins and examination of utilization of amino acid and carbon sources by skin isolates offer important information about biodegradation of salted skins. Hence, total counts of moderately halophilic bacteria, total counts of proteolytic and lipolytic moderately halophilic bacteria found on the salted sheep and goat skins belonging to different countries were examined in this study. One hundred-thirty seven moderately halophilic bacterial species closely related to species of genera Alkalibacillus, Bacillus, Chromohalobacter, Gracilibacillus, Halomonas, Idiomarina, Marinococcus, Oceanobacillus, Planococcus, Salimicrobium, Salinicoccus, Staphylococcus and Salinivibrio were used to detect utilization of different amino acids and carbon sources which are related to biodegradation of skins. The values of pH, moisture contents, ash contents and salt saturations of these skins were also investigated to understand the correlation between these parameters and moderately halophilic bacterial activities. All salted skin samples contained moderately halophilic bacteria, proteolytic and lipolytic moderately halophilic bacteria in high numbers. Each of 137 test isolates, obtained from the salted sheep and goat skins, used different amino acids found in the skin structure. While 100% of both sheep and goat skin isolates utilized L-arginine, 86%, 66%, 85%, 64% and 66% of the isolates respectively utilized L-glycine, L-alanine, L-tyrosine, L-proline and L-hydroxyproline amino acids. Ninety-three percent of the isolates used different carbon sources such as dulcitol (13%), D-sorbitol (29%), L-rhamnose (19%), ribose (52%), salicin (40%), myo-inositol (27%), xylitol (21%), benzoate (18%), propionate (34%), D-melezitose (31%), butanol (20%), propanol (23%), methanol (23%), formate (23%) and tartrate (12%). While pH values, moisture contents, ash contents and salt saturations of the sheep skins ranged from 6.53 to 8.01, 32 to 68%, 12 to 30% and 58 to 100%, respectively, the pH values, moisture contents, ash contents and salt saturations of the goat skins were between 6.65-8.06, 34-70%, 11-32% and 64-100%, respectively. The values of all skin samples were found to be suitable for the growth and catabolic activities of these bacteria. To prevent the growth and activities of moderately halophilic isolates causing skin deterioration, the annihilation effects of 0.5, 1.0, 1.5 and 2.0 A direct and alternating electric current treatments on the mixed culture of moderately halophilic isolates (Chromohalobacter israelensis, Chromohalobacter canadensis, Halomonas halodenitrificans, Staphylococcus nepalensis and Halomonas halmophila) were also separately investigated in the present study. Both 0.5, 1.0, 1.5, 2.0 A direct and alternating electric current applications used in this study completely annihilated the mixed culture of moderately halophilic spoilage microorganisms. As a conclusion, we suggest using lowlevel direct or alternating electric current treatment to eradicate harmful moderately halophilic bacteria in salt which will be used in brine curing of hides and skins in the leather industry. Note de contenu : - EXPERIMENTAL : Skin Samples - Determination of total of moderately halophilic bacteria on the skin samples - Determination of total counts of proteolytic and lipolytic moderately halophilic bacteria on the skins - Utilization of different amino acids and different carbon sources - Determination of pH values of the salted skins - Determination of moisture content, ash content and salt saturation of the salted skins - Application of alternating and direct electric currents on the mixed culture - RESULTS AND DISCUSSION : Moderately halophilic bacteria, proteolytic and lipolytic bacteria on salted sheep and goat skin samples - Determination of pH value, moisture content, ash content and salt saturation of the salted skins - Utilization of different amino acids and different carbon sources - Application of alternating and direct electric currents on the mixed culture of moderately halophilic spoilage bacteria En ligne : https://drive.google.com/file/d/13FYlhwuWlVz9ABkOdZFqz5LbN0xD_nh_/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=29950 [article]

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Molecular identification of moderately halophilic bacteria and extremely halophilic archaea isolated from salted sheep skins containing red and yellow discolorations / C. Akpolat in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CX, N° 7 (07/2015)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CX, N° 7 (07/2015) . - p. 211-220 Titre : Molecular identification of moderately halophilic bacteria and extremely halophilic archaea isolated from salted sheep skins containing red and yellow discolorations Type de document : texte imprimé Auteurs : C. Akpolat, Auteur ; Antonio Ventosa, Auteur ; Meral Birbir, Auteur ; Cristina Sánchez-Porro, Auteur ; Pinar Caglayan, Auteur Année de publication : 2015 Article en page(s) : p. 211-220 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Cuirs et peaux -- Analyse Cuirs et peaux de moutons Décoloration Peaux brutes -- Détérioration Peaux brutes -- Salage Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Salted hides or skins containing red and yellow discolorations have been examined for many years, but much less information is available about the isolation and molecular techniques for identifying moderately halophilic bacteria and extremely halophilic archaea on the salted sheep skins exhibiting these blotches. The deteriorated salted sheep skins were collected from a warehouse in Spain. Moderately halophilic bacteria and extremely halophilic archaea were isolated from these samples and molecular identification of these microorganisms were performed using 16S rRNA gene sequence analysis. Total cell counts of moderately halophilic bacteria and extremely halophilic archaea were found as 105-108 CFU/g and 105-107 CFU/g, respectively. According to comparative partial 16S rRNA gene sequence analysis, Alkalibacillus halophilus, Pseudomonas halophila, Acinetobacter johnsonii, Alkalibacillus salilacus, Salimicrobium salexigens, Marinococcus luteus and Staphylococcus equorum subsp. equorum belonging to moderately halophilic bacteria; and Halorubrum tebenquichense, Halorubrum saccharovorum, Halococcus dombrowskii, Halococcus qingdaonensis, Natrinema pellirubrum, Halococcus morrhuae, Halorubrum kocurii, Halorubrum terrestre, Halorubrum lipolyticum, Halostagnicola larsenii, Haloterrigena saccharevitans and Natrinema versiforme belonging to extremely halophilic archaea were isolated from these sheep skins. Alkalibacillus halophilus belonging to moderately halophilic bacteria and Halorubrum tebenquichense belonging to extremely halophilic archaea were found as the most common species on the skins. Among the moderately halophilic bacterial isolates, Acinetobacter johnsonii showed lipolytic activities. Among the extremely halophilic archaeal isolates, Halococcus dombrowskii, Halococcus morrhuae, Natrinema pellirubrum, Halorubrum lipolyticum showed proteolytic activity and Halococcus dombrowskii, Halorubrum lipolyticum, Haloterrigena saccharevitans, Natrinema versiforme showed lipolytic activity. Hair slip, red and yellow discolorations, slimy layers and bad odor were detected on the skin samples examined. This study confirmed that salted sheep skins were contaminated by preservation salt containing different proteolytic or lipolytic species of mostly extremely halophilic archaea. Therefore, antimicrobial applications during brine curing of skins should be applied to overcome halophilic microbial damage on the salted skins. Note de contenu : - Collection of salted skins - Determination of pH values of the salted skins - Determination of moisture content of the salted skins - Determination of total bacterial population of moderately halohilic bacteria and extremely halophilic archaea on the skin samples - Morphological, cultural and physiological characteristics of the isolates - Determination of protease activity - Determination of lipase production - Phylogenetic analysis - Nucleotide accession En ligne : https://drive.google.com/file/d/1_NYcWNCVWPoaRDNsUI_pJ6gxrv5e2xHc/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24374 [article]

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Screening of industrially important enzymes produced by moderately halophilic bacteria isolated from salted sheep skins of diverse origin / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXII, N° 6 (06/2017)  

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The effects of alternating and direct electric currents on moderately halohilic bacteria in leather industry / Pinar Caglayan in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 100, N° 6 (11-12/2016)  

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