[article]
Titre : |
The effects of alternating and direct electric currents on moderately halohilic bacteria in leather industry |
Type de document : |
texte imprimé |
Auteurs : |
Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Ayse Ogan, Auteur ; Antonio Ventosa, Auteur ; Cristina Sánchez-Porro, Auteur ; Yasar Birbir, Auteur |
Année de publication : |
2016 |
Article en page(s) : |
p. 307-313 |
Note générale : |
Bibliogr. |
Langues : |
Anglais (eng) |
Catégories : |
Antimicrobiens Bactéries -- Inactivation Courants électriques Cuirs et peaux -- Conservation Cuirs et peaux de moutons
|
Index. décimale : |
675 Technologie du cuir et de la fourrure |
Résumé : |
Proteolytic and lipolytic moderately halophilic bacteria, originate from salt, may be found on salted sheep skins. Microbial spoilage of sheepskin usually result a from the failure to inactivate proteolytic and Iipolytic micro-organisms during skin preservation. To solve this problem, an effective inactivation method to be used in the leather industry was examined in this study. Protease and lipase activities of moderately halophilic Staphylococcus saprophyticus subsp. saprophyticus (TR5), Bacilius pumilus (DB5), Bacillus licheniformis (DBA2), Gracilibacillus dipsosauri (EK2) and Idiomarina loihiensis (GA3), isolated from salted sheepskins, were examined : Protease activity of each test isolate was determined by bath gelatin agar medium and casein digestion assay at 280nm. Lipolytic activity of each test isolate was determined by bath Tween 80 agar medium and measuring hydrolysis of p-nitrophenylbutyrate to p-nitrophenol at 405nm. Antibiotic sensitivities of test isolates to novobiocin, penicillin G, tetracycline, streptomycin, erythromycin, tobramycin, kanamycin and gentamicin were tested using disc diffusion susceptibility method. These isolates were found to be proteolytic, lipolytic and resistant to aforementioned antibiotics. inactivation effects of direct electric currents and alternating electric currents (0.5A, 1.0A, 1: 5A and 2.0A) on the mixed culture of these isolates were separately examined in liquid medium containing 25% NaCl. The mixed culture was killed in 1 minute by 0.5A direct current and 1.5A affernating current treatments. However, 0.5A alternating current and 1.0A alternating current treatments eliminated these micro-organisms respectively in 15 minutes and 10 minutes. In conclusion, electric current applications hold great potentiel for the eradication of proteolytic and lipolytic, antibiotic-resistant, moderately halophilic bacteria in leather industry. |
Note de contenu : |
- EXPERIMENTAL PROCEDURES : Moderately halophilic bacterial isolates - Proteolytic and lipolytic activities of moderately halophilic bacteria - Antimicrobial susceptibility test - The electrochemical treatment system
- RESULTS AND DISCUSSION : Antibiotic resistance |
En ligne : |
https://drive.google.com/file/d/1694CJoD9Uwle2gArKMHAUoJyAkxmVqSI/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27666 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 100, N° 6 (11-12/2016) . - p. 307-313
[article]
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