| Titre : |
Assessment of penetration and permeation of caffeine by confocal Raman spectroscopy in vivo and ex vivo by tape stripping |
| Type de document : |
document électronique |
| Auteurs : |
Ghaith Kourbaj, Auteur ; Annette Gaiser, Auteur ; Stephan Bielfeldt, Auteur ; Dominique Lunter, Auteur |
| Année de publication : |
2023 |
| Article en page(s) : |
p. 14-28 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Caféine
Décapage
Pénétration cutanée
Perméation
Rubans adhésifs
Spectroscopie Raman
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : Tape stripping is an often-used non-invasive destructive method to investigate the skin penetration of a substance. In recent years, however, the suitability of confocal Raman spectroscopy (CRS) as a non-invasive method of non-destructive examination of the skin has become increasingly apparent. In this study, we compared invasion and depletion penetration and permeation kinetics of a 2% caffeine solution with and without 1,2-pentanediol as a penetration enhancer measured with CRS and tape stripping.
- Methods : Porcine skin was used for tape stripping and human skin for CRS. 2% caffeine solution was applied to the skin for different incubation times. Human skin was then examined by CRS while caffeine was extracted from porcine skin and quantified via reverse-phase HPLC. Fluxes were also measured and calculated by sum of the total amounts of caffeine penetrated into the skin.
- Results : Without penetration enhancers, there is hardly any difference between the penetration profiles of the two measurement methods for invasion, but the curves for depletion are different. Furthermore, the calculated flux values for the invasion are almost identical, but for the depletion the tape stripping values are about twice as high as the CRS values.
- Conclusion : The relevance of conducting invasion and depletion studies became clear and was able to show the still existing problems in the comparability of CRS and tape stripping. |
| Note de contenu : |
- MATERIALS AND METHODS : Materials - Test solutions - Ex vivo tape stripping - In vivo confocal Raman spectroscopy - Statistics
- RESULTS : Results of ex vivo tape stripping - Results of in vivo CRS measurements
- DISCUSSION : Tape stripping results
- DISCUSSION OF THE CRS RESULTS : Comparing tape stripping results to CRS results
- Table 1 : Make-up of test solutions
- Table 2 : Incubation times
- Table 3 : Flux from different experimental setups
- Table 4 : Flux from different experimental setups. Flux over 1 h from different experimental setups |
| DOI : |
https://doi.org/10.1111/ics.12820 |
| En ligne : |
https://drive.google.com/file/d/144nCOFKEh8YNpyXDssgQ2dBq7xZHp7Ff/view?usp=share [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38816 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 1 (02/2023) . - p. 14-28
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