Enzymatic hydrolysis of skin shavings for preparation of collagen hydrolysates with specified molecular weight distribution / Chi Yuanlong in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 96, N° 1 (01-02/2012)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 96, N° 1 (01-02/2012) . - p. 16-20 Titre : Enzymatic hydrolysis of skin shavings for preparation of collagen hydrolysates with specified molecular weight distribution Type de document : texte imprimé Auteurs : Chi Yuanlong, Auteur ; Cui Min, Auteur ; Cui Xiaoju, Auteur ; Wenhua Zhang, Auteur ; Xuepin Liao, Auteur ; Bi Shi, Auteur Année de publication : 2012 Article en page(s) : p. 16-20 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Collagène Cuirs et peaux -- Déchets Hydrolysats de protéines Hydrolyse enzymatique Tags : 'Copeaux  tannerie'  'Protéase  1398'  2709'  Alcalase  Papaïne  Pepsine  537'  Trypsine  'Titration  formaldehyde'  Electrophorèse  'Ges  dodécyl  sulfate-polyacrylamide  sodium'  'Ultrafiltration  centrifuge' Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Collagen hydrolysates were extracted from tannery skin shavings through enzymatic hydrolysis by using protease 1398, protease 2709, alcalase, papain, pepsin, protease 537 and trypsin, respectively. The hydrolytic degree and molecular weight distribution (MWD) of the hydrolysages were evaluated by the formaldehyde titration method, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), tricine-SDS-PAGE and centrifugal ultrafiltration. It was found that the MWD of the as prepared collagen hydrolysates has a close correlation with the hydrolytic degree, and greatly depends on the enzyme employed. The low molecular weight (MW) collagen hydrolysates, with relatively high hydrolytic degree, were obtained by enzymatic hydrolysis using protease 1398, protease 2709, papain and alcalase. More than 60% (mass ratio) of the fractions in these four hydrolysates were in the molecular weight range of <10kDa. The use of protease 537 produced medium-MW collagen hydrolysages where 40% of the fractionswere of 10-30kDa. The high-MW collagen hydrolysages that include about 66% fractions with molecular weights higher than 20kDa were obtained by using pepsin, accompanied by the lowest hydrolytic degree. This research provides a potential approache for molecular weight and molecular weight distribution control collagen hydrolysates by enzymatic hydrolysis ot tannery skin wastes. Note de contenu : - MATERIALS AND METHODS : Materials - Methods - Determination of hydrolytic degree of collagen - SDS-PAGE and tricine-SDS-PAGE determination - Centrifugal ultrafiltration analysis - RESULTS AND DISCUSSION : Hydrolytic degree analysis - SDS-PAGE and tricine-SDS-PAGE patterns - Centrifugal ultrafiltration analysis on MWD En ligne : https://drive.google.com/file/d/1NEe4OCKiTxbrMEX3emn2sQFAzagsFJQc/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=13549 [article]

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Raw skin wastes - used to prepare a flocculant for the treatment of black liquor for papermaking / Chi Yuanlong in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 95, N° 5 (09-10/2011)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 95, N° 5 (09-10/2011) . - p. 209-214 Titre : Raw skin wastes - used to prepare a flocculant for the treatment of black liquor for papermaking Type de document : texte imprimé Auteurs : Chi Yuanlong, Auteur ; LV Sining, Auteur ; Qiang He, Auteur ; Xuepin Liao, Auteur ; Wenhua Zhang, Auteur ; Bi Shi, Auteur Année de publication : 2011 Article en page(s) : p. 209-214 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Collagène Cuirs et peaux -- Déchets -- Recyclage Floculant Fourier, Spectroscopie infrarouge à transformée de Papier Potentiel zeta Procédés de fabrication Spectroscopie de photoélectrons Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : A type of novel flocculating agent, collagen protein flocculant (CPF), was prepared by using untanned skin waste from a tannery as raw material and, as a typical application, the flocculation behaviour of CPF on papermaking black liquor was investigated. Four CPFs with different degrees of cationicity were synthesized and their structures were characterized by FT-IR, XPS, zeta potential analysis, and the determinations of degree of cationization and primary amine content. The CPFs exhibited satisfactory flocculation on papermaking black liquor because they led to ghigh TOC and colour removals and produced a small volume of sludge. The effective flocculation behaviour of CPFs can be attributed to a combined effect of charge neutralization and hydrogen bonding between CPF and the lignin in black liquor, as well as the binding of collagen to lignin. This research provides a novel approach in reuse of the skin wastes from leather industry. Note de contenu : - Materials - Preparation of CPF - Characterization of CPF - Flocculation of CPFs to papermaking black liquor En ligne : https://drive.google.com/file/d/1kF5A2mFMSuNPZgqCd-aCZV_yZA1UliBe/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=12221 [article]

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