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Titre : An all-round winner : High-Tech production of life-saving disks for fast analysis of dangerous Germs Type de document : texte imprimé Auteurs : Martin Manka, Auteur Année de publication : 2021 Article en page(s) : p. 14-15 Langues : Anglais (eng) Catégories : Diagnostic biologique
Matériel médical
Matières plastiques -- Moulage par injection
Polymères en médecine
Salles blanches
Technologie médicaleIndex. décimale : 668.5 Parfums et cosmétiques Résumé : RKT has been manufacturing plastic products for medical and industrial technology for 45 years. Discs for the rapid analysis of multi-resistant hospital germs are a lighthouse project within the sophisticated product portfolio. Note de contenu : - Channels measured in microns
- Own department for process development
- Fig. 1 : A six-axis robot picks the molded parts out of the mold under an inert gas atmosphere and conveys them into the cleanroom for further processing and packaging
- Fig. 2 : Here, a disk is filled with a spherical freeze-dried reagent (lyophilisate)
- Fig. 3 : A cover is clipped onto the disk (the circular sample inlet at the front) to protect the filmEn ligne : https://drive.google.com/file/d/10tASbCw4hXW1EOkt-25vVq3zhPdxWcrG/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35532
in KUNSTSTOFFE INTERNATIONAL > Vol. 111, N° 3 (2021) . - p. 14-15[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 22639 - Périodique Bibliothèque principale Documentaires Disponible Bright fluorescent dsDNA probes : novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine-quinolinium chromophore / Aleksey Vasilev in COLORATION TECHNOLOGY, Vol. 131, N° 2 (04/2015)
Titre : Bright fluorescent dsDNA probes : novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine-quinolinium chromophore Type de document : texte imprimé Auteurs : Aleksey Vasilev, Auteur ; Nedyalko Lesev, Auteur ; Sonya Dimitrova, Auteur ; Marina Nedelcheva-Veleva, Auteur ; Stoyno Stoynov, Auteur ; Silvia Angelova, Auteur Année de publication : 2015 Article en page(s) : p. 94-103 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : ADN
Analyse spectrale
Colorants
Colorants -- Synthèse
Diagnostic biologique
Electrophorèse
FluorescenceIndex. décimale : 667.3 Teinture et impression des tissus Résumé : Seven tri- and tetracationic monomeric and homodimeric monomethine cyanine dyes based on thiazolo[4,5-b]pyridinium and quinolinium end groups were synthesised and characterised. The dyes were tested as fluorescent DNA intercalating probes to apply in DNA gel electrophoresis. The DNA samples stained with all dyes from the series demonstrated bright fluorescent signals. DNA fragments were successfully visualised under orange and green filters as well as under standard UV transillumination. Two of the studied dyes revealed higher sensitivity to DNA when compared with the commercial dimeric cyanine dye TOTO-1. Their sensitivity reached that of the commercial dimeric cyanine dye YOYO-1, but the emission was shifted to longer wavelengths. These qualities make the dyes suitable to apply in a wide range of medical and scientific analytical methods. Note de contenu : - EXPERIMENTAL : Materials - Equipment - Synthesis of dye 3 - Synthesis of tri- and tetracationic dyes 5a to 5g - Preparation of dyes and DNA solutions - Staining after electrophoresis - Staining prior to electrophoresis
- RESULTS AND DISCUSSION : Synthesis and structural characterisation of dyes 5a to 5g - Spectroscopic characteristics of the dyes and computational study of the chromophore before binding to dsDNA - Spectroscopic characteristics after binding to dsDNA - Staining after electrophoresis completion - Staining DNA samples prior to electrophoresis - Retardation of the mobility of stained DNA fragmentsDOI : 10.1111/cote.12133 En ligne : https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12133 Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=23656
in COLORATION TECHNOLOGY > Vol. 131, N° 2 (04/2015) . - p. 94-103[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 17121 - Périodique Bibliothèque principale Documentaires Disponible
Titre : Development and application of artificial intelligence-based facial skin image diagnosis system: Changes in facial skin characteristics with ageing in Korean women Type de document : texte imprimé Auteurs : Hyeokgon Park, Auteur ; Sae-ra Park, Auteur ; Sangran Lee, Auteur ; Joongwon Hwang, Auteur ; Myeongryeol Lee, Auteur ; Sue Im Jang, Auteur ; Yuchul Jung, Auteur ; Yeongmin Yeon, Auteur ; Nayoung Kang, Auteur ; Byung-Fhy Suh, Auteur ; Eunjoo Kim, Auteur Année de publication : 2024 Article en page(s) : p. 199-208 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Coréen(ne)s
Diagnostic biologique
Imagerie (technique)
Intelligence artificielle
Peau -- analyse
Peau féminineIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : To develop and validate an artificial intelligence (AI)-based diagnostic system for analysing facial skin images using expert judgements and explore its feasibility for skin ageing research, specifically by evaluating facial skin changes in Korean women of various ages.
- Methods : Our AI-based facial skin diagnosis system (Dr. AMORE®) uses facial images of Korean women to analyse wrinkles, pigmentation, skin pores, and other skin red spots. The system is trained using clinical expert evaluations and deep learning. We assessed the system's precision and sensitivity by analysing the correlation between the diagnoses by the AI system and those of the experts. We used 120 images of Korean women aged 10–60 years to evaluate the changes in various facial skin characteristics with ageing.
- Results : The precision and sensitivity of the developed system were excellent (>0.9%), and the diagnosis scores using the detected area and intensity of each item were correlated significantly higher with the visual evaluation results of the clinical experts (>0.8, p < 0.001). We also analysed facial images of Korean women aged 10–60 years to quantify changes in the scores of wrinkles, pigmentation, and skin pores with age. We identified the age group with the most significant changes as 20s to 30s. Analysis of the detailed skin characteristics of each item showed that wrinkles and pigmentation changed significantly in the 20s–30s, and skin pores increased significantly in the 10s–20s. There was no significant correlation with age or change according to the age group for skin red spots.
- Conclusion : Developed AI-based facial skin diagnosis system can automatically diagnose skin conditions based on clinical expert judgement using only photographic images and analyse various items in detail, quantitatively, and visually. This AI system can provide new and useful approaches in research areas that require a lot of resources and different characterizations, such as the study of facial skin ageing.Note de contenu : - Collecting facial skin images
- Image data preparation for AI training
- Designing and training an AI skin diagnosis system
- Validation of an AI skin diagnosis system
- Evaluation of an AI skin diagnostic system's analysis of changes in skin ageing characteristics
- Image and statistical analysis
- Table 1 : Detailed diagnostic items of AI skin diagnosis system.
- Table 2 : Age distribution of Korean female participants to analyse changes in skin ageing characteristics
- Table 3 : Results of clinical expert evaluation of detection visualization images of AI skin diagnosis system
- Table 4 : Correlation coefficients and significance probabilities of score results for each skin diagnosis item between the clinical experts and the AI skin diagnosis system
- Table 5 : Changes in facial skin diagnostic scores according to age in Korean women, correlation coefficients and probability of significance with age, and probability of significant change from adjacent age groups
- Table 6 : Changes in facial skin detailed diagnostic items according to age in Korean women, correlation coefficients and probability of significance with age, and probability of significant change from adjacent age groupsDOI : https://doi.org/10.1111/ics.12924 En ligne : https://drive.google.com/file/d/171UsYqi87UzenncJQUsWSAdMU-gNRqTY/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40907
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 46, N° 2 (04/2024) . - p. 199-208[article]Exemplaires
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Titre : Development of a skin microbiome diagnostic method to assess skin condition in healthy individuals : Application of research on skin microbiomes and skin condition Type de document : document électronique Auteurs : Yuki Kotakeyama, Auteur ; Rie Nakamura, Auteur ; Masaharu Kurosawa, Auteur ; Seiko Ota, Auteur ; Ruka Suzuki, Auteur ; Miki Nakanishi, Auteur ; Kohei Kanno, Auteur ; Kosuke Watanabe, Auteur ; Yukiko Ishitsuka, Auteur Année de publication : 2021 Article en page(s) : p. 677-690 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Acné L'acné (nom féminin) est une dermatose (maladie de la peau) inflammatoire des follicules pilosébacés (glandes sécrétant le sébum, à la racine des poils) avec formation de comédons.
Cette maladie de la peau est représentée dans sa forme la plus fréquente par l'acné polymorphe juvénile qui touche les adolescents, mais qui ne résume pas toutes les acnés. Il existe aussi des acnés exogènes d'origine professionnelle, médicamenteuse, cosmétique...
Dermo-cosmétologie
Diagnostic biologique
Microbiologie
Microbiome -- Analyse
Microbiome cutané
Peau -- Physiologie
Peau -- Rides
Peau -- Soins et hygiène
Pellicules (dermatologie)
RosacéeLa rosacée, connue aussi sous le nom de couperose, est une maladie cutanée incurable au départ bénigne qui se manifeste par des rougeurs chroniques au niveau du nez, des joues, parfois aussi au niveau du menton et du front. Ces symptômes s'accompagnent d'une sensation de picotement, notamment au niveau des yeux. De petits vaisseaux sanguins, caractéristiques des varicosités sont souvent visibles dans les zones touchées associées à des télangiectasies.
La maladie est évolutive et peut provoquer des crises d'acné, notamment en cas de stress ou de fatigue, et, à un stade avancé, faire enfler le nez du sujet qui reste rouge et bosselé (rhinophyma). Lorsque la rosacée est à un stade plus avancé, ou soumise à différents facteurs, elle peut aussi durement affecter la vue du sujet qui en est atteint et un examen dermatologique est recommandé.
Celle maladie se distingue de l'érythrose faciale qui est un érythème diffus disparaissant à la vitropression.
StatistiqueIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Skin microbiomes vary across individuals. They are known to play essential roles in maintaining homeostasis and preventing infectious pathogens. In recent years, cosmetic product development has begun to focus on the relationship between skin microbiomes and skin conditions. However, the statistical methods used in many studies include the standard t-test and small-scale correlation analysis, which do not take into account the internal correlation structure in data on skin microbiomes and skin features. In this study, we aimed to understand the relationship between skin microbiomes and skin features by analysing complex microbiomes and skin data.
- Methods : We obtained data on 19 skin characteristics and skin microbiomes based on 16s ribosomal RNA (16S rRNA) gene analysis of 276 healthy Japanese women. We then performed the principal component analysis (PCA), a method that takes into account the internal correlation structure, on 234 panels of them that did not contain outliers or missing values. We confirmed the relationship between skin microbiomes and skin features with principal component regression analysis and hierarchical clustering analysis (HCA).
- Results : The principal component regression analysis showed strong relationships between skin microbiomes and sebum-related skin characteristics and skin pH. In the HCA, the female panel was classified into two major groups based on the skin microbiome. Furthermore, there were significant differences in sebum-related skin characteristics and the way skin condition changes with ageing between those groups, suggesting the possibility of measuring skin condition and age-related skin risk based on microbiome data. In addition, sebum-related characteristics differed significantly among middle-aged participants, suggesting a strong relationship between skin microbiomes and sebum-related characteristics.
- Conclusion : Analysis of skin condition and skin microbiome in Japanese women, taking into account the correlation between variables, showed that skin microbiome was significantly related to the number of pores and the amount of sebum. Furthermore, it was suggested that the skin condition and the way the skin condition changes with ageing may differ depending on the type of skin microbiome. The finding of a relationship between skin condition and skin microbiome suggests the possibility of proposing a new beauty method focusing on the skin microbiome in the future.Note de contenu : - SUBJECTS AND METHODS : Study population - Acquisition of data on skin features - Acquisition of skin microbiome data and calculation - Computational analysis - Selection of microbiome genera for analysis - Statistical analysisPrincipal component analysis - Multivariable linear regression on skin features and skin microbiomes - Hierarchical clustering analysis and Mann–Whitney's analysis on skin features and skin microbiomes
- RESULTS : Study population - Statistical analysis
- Table 1 : Age and skin features of the study participants
- Table 2 : Pearson's correlations derived from the principal component analysis of 19 skin features
- Table 3 : Variances derived from the principal component analysis (PCA) of 19 skin features
- Table 4 : Rotated components: eigenvector between 19 skin features and 9 main principal components (PCs)
- Table 5 : Statistical associations between the main skin microbiome and skin feature score derived from the principal component analysis
- Table 6 : Comparison of each skin feature by group (Mann– Whitney U test)
- Table 7 : Comparison of each skin feature by group while taking age bias into account (Mann– Whitney U test)
- Table 8 : Correlations between age and skin features in all panels and by microbiome typeDOI : https://doi.org/10.1111/ics.12744 En ligne : https://drive.google.com/file/d/1ZvqY0QBBQSJcfM312AXpBvj9A3pruCs7/view?usp=shari [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37049
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 6 (12/2021) . - p. 677-690[article]Exemplaires
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Titre : Hue-shifting for accurate and precise quantification of biochemical substances using diagnostic test strips Type de document : texte imprimé Auteurs : Ryoichi Doi, Auteur Année de publication : 2021 Article en page(s) : p. 530-543 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Analyse quantitative (chimie)
Biochimie
Couleur
Diagnostic biologique
Statistique
Tests biologiquesIndex. décimale : 667.3 Teinture et impression des tissus Résumé : Some test strips have high feasibility for the quick and approximate quantification of the biochemical substances in samples. Recently, test strips became more precise and accurate by involving the quantitative observation of coloration and regression modelling. Their accuracy and precision can be significantly improved by hue-shifting the colours of the original sample and those of the standards. The standard colour images included in the instructions of test strips for the detection of four urinary and two salivary biochemical substances were used. Images of glucose-loaded test strips for urinary glucose detection were also prepared. Using the red-green-blue (RGB) colour image, the author prepared 11 hue-shifted colour images by changing the hue of the original RGB colour image at intervals of 30°. From each of the 12 colour images, 10 greyscale images indicating values of the intensity of RGB, cyan, magenta, yellow, key black, L*, a* and b* were prepared. In regression modelling, the additional 110 greyscale images improved the values of the coefficient of determination and normalised mean absolute error in the quantification of protein, specific gravity, urobilinogen and glucose in urine, as well as of alcohol and nitric oxide in saliva. Further analyses showed that the hue-shifting technique yielded new greyscale images that were more significantly related to the values of the biochemical variables in question than any previous greyscale images. This improvement was realised by the most significant linear measures for the description of concentrations of biochemical substances, and synergetic effects between significant greyscale images. Note de contenu : - Test strip products for urine and saliva
- Processing images of standard colours
- Statistical analyses
- Table 1 : Multiple regression statistics for significant greyscale images for the quantification of urinary and salivary biochemical substances with and without hue-shifting of the colour images of the standard colours in the instructions of test strip products
- Table 2 : Linear correlations between concentrations of the urinary and salivary biochemical substances and the greyscale intensity for the
pixels (n = 10) in the 10 greyscale images without hue-shifting or the significant greyscale images derived from the hue-shifted colour imagesDOI : https://doi.org/10.1111/cote.12551 En ligne : https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12551 Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=36510
in COLORATION TECHNOLOGY > Vol. 137, N° 5 (10/2021) . - p. 530-543[article]Réservation
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