Characterization and control of antibiotic-resistant calmati saltern's isolates with bacteriocins / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVII, N° 8 (08/2022)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVII, N° 8 (08/2022) . - p. 315-329 Titre : Characterization and control of antibiotic-resistant calmati saltern's isolates with bacteriocins Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur Année de publication : 2022 Article en page(s) : p. 315-329 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries -- Isolement Bactéries halophiles Bactériocines Les bactériocines sont une famille de peptides ou protéines synthétisés naturellement par certaines bactéries. Une bactériocine consiste généralement en un composé protéique de 20 à 60 acides aminés. Les bactériocines ne sont pas des antibiotiques mais elles possèdent des propriétés antibiotiques : - Elles peuvent être bactériocides, c'est-à-dire éliminer certains micro-organismes. - Elles peuvent être bactériostatiques, c'est-à-dire inhiber la croissance de certains micro-organismes. Caractérisation Concentration bactéricide minimale La concentration bactéricide minimale (CBM) est la plus faible concentration d'un agent antibactérien nécessaire pour tuer une bactérie particulière1. Elle peut être déterminée à partir des tests de concentration minimale inhibitrice (CMI) de dilution en bouillon en repiquant les bactéries sur des plaques d'agar qui ne contiennent pas l'agent antibactérien que l'on teste. La CBM est identifiée en déterminant la concentration la plus faible d'agent antibactérien qui réduit la viabilité de l'inoculum bactérien initial à un niveau supérieur ou égal à 99,9%. La CBM est complémentaire à la CMI ; alors que le test CMI indique le niveau le plus bas d'agent antimicrobien qui inhibe la croissance, le CBM indique le niveau le plus bas d'agent antimicrobien qui entraîne la mort microbienne. Cela signifie que même si une CMI particulière montre une inhibition, le repiquage des bactéries sur de la gélose peut encore entraîner la prolifération de l'organisme car l'antimicrobien n'a pas causé la mort. Les agents antibactériens sont généralement considérés comme bactéricides si la CBM ne dépasse pas quatre fois la CMI. Comme le test CBM utilise des unités formant colonies comme mesure indirecte de la viabilité bactérienne, il peut être biaisé par des agents antibactériens qui provoquent l'agrégation des cellules bactériennes. On peut citer en exemples les flavonoïdes et les peptides. (Wikipedia) Concentration minimale inhibitrice (antimicrobiens) En microbiologie, la concentration minimale inhibitrice (CMI) est la plus faible concentration d'un produit chimique, généralement un médicament, qui empêche la croissance visible d'une ou de plusieurs bactéries. La CMI dépend du micro-organisme considéré, de l'être humain affecté (in vivo uniquement) et de l'antibiotique lui-même. La CMI est déterminée en préparant des solutions du produit chimique in vitro à diverses concentrations croissantes, en incubant les solutions avec des groupes séparés de bactéries en culture et en mesurant les résultats en utilisant une méthode de dilution standardisée (agar ou microdilution). Les résultats se classent ensuite comme "sensible", "intermédiaire" ou "résistant" à un antimicrobien particulier en utilisant un point d'arrêt. Les points d'arrêt sont des valeurs convenues, publiées dans les directives d'un organisme de référence, comme le US Clinical and Laboratory Standards Institute (CLSI), la British Society for Antimicrobial Chemotherapy (BSAC) ou le Comité européen sur les tests de sensibilité aux antimicrobiens (EUCAST). On a pu constater des écarts importants au niveau des points d'arrêt de divers pays européens au fil des ans, et entre ceux de l'EUCAST et du CLSI. Alors que la CMI est la concentration la plus faible d'un agent antibactérien nécessaire pour inhiber la croissance visible, la concentration bactéricide minimale (CBM) est la concentration minimale d'un agent antibactérien qui entraîne la mort bactérienne. Plus la CMI est proche de la CBM, plus le composé est bactéricide. La première étape de la découverte d'un médicament est souvent le dépistage d'un médicament candidat de banque de données pour les CMI contre les bactéries d'intérêt. En tant que tels, les CMI sont généralement le point de départ pour de plus grandes évaluations précliniques de nouveaux agents antimicrobiens. Le but de la mesure de la concentration minimale inhibitrice est de s'assurer que les antibiotiques sont choisis efficacement pour augmenter le succès du traitement. (Wikipedia) Microbiologie Résistances aux antibiotiques Sels de sodium Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Camalti Saltern is the largest solar saltern in Izmir, Turkey. The salt obtained from Camalti Saltern is used in food and leather industries to prevent bacterial growth. In this saltern, seawater is pumped into shallow ponds. Then, the sun and wind cause evaporation and crystallization, finally sea salt is produced. Due to the fact that Camalti Saltern contains various halophilic bacteria, the goals of the present study were to isolate and identify haloversatile bacteria from Camalti Saltern’s brine samples, to examine their antibiotic resistance profiles, to determine antimicrobial activities under optimum environmental conditions, to determine bacteriocin concentration by Bradford Method, to detect Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) of bacteriocins against multidrug-resistant isolates, and to observe the cell structure of bacteriocin-treated bacteria under SEM. Sixteen bacterial isolates were recovered from Camalti Saltern’s brine samples and were identified as 14 different species (Bacillus haynesii, Bacillus simplex, Bacillus subtilis subsp. stercoris, Bacillus pumilus,Staphylococcus petrasii subsp. jettensis, Staphylococcus saprophyticus subsp. saprophyticus, Kocuria sediminis, Rhodococcus enclensis, Marinobacter hydrocarbonoclasticus, Vibrio olivae, Marinomonas communis, Pseudomonas psychrotolerans, Salinivibrio costicola subsp. vallismortis, Vibrio neocaledonicus). Percentages of antibiotic resistance of isolates were 63% to aztreonam, 50% to amoxicillin/clavulanic acid, 44% to ampicillin, 44% to cefadroxil, 31% to imipenem, 19% to ampicillin/sulbactam, 6% to chloramphenicol, 6% to tetracycline, 6% to mupirocin, 6% to meropenem. The bacteriocin concentrations of Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis were measured as 1.02 mg/mL and 1.25 mg/mL, respectively. Bacteriocins of Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis, which were not resistant to any antibiotics tested,exhibited the inhibitory effect against Kocuria sediminis resistant to ten antibiotics and Bacillus pumilus resistant to four antibiotics. Bacteriocin of Salinivibrio costicola subsp. vallismortis also demonstrated the inhibitory effect against Pseudomonas psychrotolerans resistant to five antibiotics. Scanning electron micrographs showed that cell morphologies of bacteriocin-treated isolates (Kocuria sediminis, Bacillus pumilus, Pseudomonas psychrotolerans) were damaged. In conclusion, bacteriocins produced from the haloversatile Camalti saltern isolates may be used in the leather industry to prevent the growth of antibiotic-resistant haloversatile bacteria. Note de contenu : - Sample collection - Isolation of bacteria - Characterization of the isolates - Amplification and sequencing of 16S rRNA genes - Nucleotide accession number - Antibiotic susceptibility - Preparation of cell-free supernatant - Screening of antimicrobial activity among the test isolates - Determination of protein concentration using Bradford method - Optimum conditions for bacteriocin production from Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Effect of heat on bacteriocins produced by Rhodococcus enclensis and Salnivibrio costicola subsp. vallismortis - Effect of pH on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Effect of NaCl concentration on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Effect of enzymes (Proteinase K and Lipase) on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis - Minimum inhibitory concentrations and minimum bactericidal concentrations of bacteriocins against multidrug-resistant isolates - Preparation of bacteriocin-treated and bacteriocin-untreated cells for scanning electron microscopy - Table 1 : Characteristics of haloversatile bacteria isolated from brine samples of the Camalti Saltern - Table 2 : Inhibition zone diameter measurements (mm) of haloversatile bacteria isolated from brine samples of the Camalti Saltern - Table 3 : Inhibitory effect of bacteriocin produced by bacterial isolates against each other - Table 4 : Inhibition zone diameters (mm) of multidrug-resistant haloversatile bacteria against bacteriocins obtained from Rhodococcus enclensis, Salinivibrio Costicola subsp. vallismortis at different incubation temperatures, pH and NaC1 concentrations - Table 5 : The effects of different temperatures, pH, NaC1 concentrations, proteinase K and lipase enzymes on antibacterial effect of bacteriocins - Table 6 : The Minimum Inhibitory Concentrations of bacteriocin produced from Rhodococcus enclensis against Kocuria sediminis and Bacillus pumilus - Table 7 : The Minimum Inhibitory Concentrations of bacteriocin produced from Salinivibrio costicola subsp. vallismortis against Kocuria sediminis, Bacillus pumilus and Pseudomonas psychrotolerans DOI : https://doi.org/10.34314/jalca.v117i8.5982 En ligne : https://drive.google.com/file/d/1G3HZoIp4GH7W3A22JxMjjbmRimXH0dkk/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37824 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
23516	-	Périodique	Bibliothèque principale	Documentaires	Disponible

Determination of hydrolytic enzyme capabilities of halophilic archaea isolated from hides and skins and their phenotypic and phylogenetic identification / S. T. Bilgi in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CX, N° 2 (02/2015)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CX, N° 2 (02/2015) . - p. 33-42 Titre : Determination of hydrolytic enzyme capabilities of halophilic archaea isolated from hides and skins and their phenotypic and phylogenetic identification Type de document : texte imprimé Auteurs : S. T. Bilgi, Auteur ; Binnur Meriçli Yapici, Auteur ; Smaïl Karaboz, Auteur Année de publication : 2015 Article en page(s) : p. 33-42 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Isolement Cuirs et peaux Enzymes protéolytiques Une enzyme protéolytique est une enzyme capable de couper une protéine en plusieurs fragments ou peptides. La trypsine, la papaïne, la pepsine, la chymotrypsine, la plasmine, la subtilisine... sont capables de couper une protéine, chaque enzyme étant spécifique de certains sites particuliers de cette protéine. C'est ainsi, par exemple, qu'une immunoglobuline G est découpée par la papaïne en un fragment Fc et deux fragments Fab, comme l'a montré Porter en 1959. Peaux brutes -- Salage Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : This research aims to isolate extremely halophilic archaea from salted hides, to determine the capacities of their hydrolytic enzymes, and to identify them by using phenotypic and molecular methods. Domestic and imported salted hide and skin samples obtained from eight different sources were used as the research material. 186 extremely halophilic microorganisms were isolated from salted raw hides and skins. Some biochemical, antibiotic sensitivity, pH, NaCl, temperature tolerance and quantitative and qualitative hydrolytic enzyme tests were performed on these isolates. In our study, taking into account the phenotypic findings of the research, 34 of 186 isolates were selected. These isolates were identified by 16S rRNA sequence analysis and 15 different strains of extreme halophilic archaea were identified. 13 strains of these were identified for first time from salted raw hide and skin in our study including Natrialba aegyptia, Halococcus thailandensis, Halococcus dombrowskii, Halovivax asiaticus, Halovivax sp. E107, Haloarchaeon, Natronococcus sp., Halorubrum sp., Halomicrobium zhouii, Natronococcus jeotgali, Haloterrigena thermotolerans, Natrinema versiforme, Halobacterium noricense. At the same time detecting Natrialba aegyptia in 6 of 8 hide samples showed that this strain is widely found in hide and skin samples. Research results are expected to contribute to other studies and solving microbial problems in leather industry. Note de contenu : - RESULTS : Qualitive findings concerning hydrolytic enzyme activities - Quantitative findings concerning hydrolytic enzyme activities - Molecular identification - DISCUSSION : Discussion findings concerning measurement of hydrolytic enzyme activity - Discussion of the finding concerning molecular identification En ligne : https://drive.google.com/file/d/1sR44uFApruPJWE2Pi5Ro2vFbltiwkQGk/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=23198 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
16945	-	Périodique	Bibliothèque principale	Documentaires	Disponible

Enzymatic reactions and phylogenetic analysis of haloveratile bacteria isolated from çamalti saltern salt samples used in the leather industry / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIV, N° 12 (12/2019)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIV, N° 12 (12/2019) . - p. 450-461 Titre : Enzymatic reactions and phylogenetic analysis of haloveratile bacteria isolated from çamalti saltern salt samples used in the leather industry Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur Année de publication : 2019 Article en page(s) : p. 450-461 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Isolement Cuirs et peaux -- Séchage Cuirs et peaux de moutons Enzymes microbiennes Sels de sodium Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Çamalti Saltern is one of the large seawater-based saltern located in Izmir, Turkey. The crude solar salt from Çamalti Saltern is used in leather industry. This type of salt may harbor viable microbial population which can spoil leather products during the curing process for hide/skins. Therefore, the objectives of this research were to isolate haloversatile bacteria from salt samples collected from Çamalti. Saltern, to identify these isolates using both conventional and molecular methods, and to determine their hydrolytic enzymes such as protease, lecithinase, cellulase, lipase, deoxyribonuclease, pullulanase, amylase, urease, caseinase, xylanase and esterase. In addition, damage caused by lipase and protease producing haloversatile bacteria to the structure of salted sheep skins was examined. The effects of different salt concentrations, pH and temperature values on the growth of haloversatile isolates were also investigated. Sixty-nine isolates of aerobic Bacteria showing different colony morphology, pigmentation, colony shape, and size were selected for characterization of the isolates. Phylogenetic analysis showed isolates belonging to the genera Staphylococcus (9 species), Exiguobacterium (3 species), Bacillus (11 species), Microbacterium (3 species), Gordonia (1 species), Kocuria (3 species), Paracoccus (2 species), Micrococcus (2 species), Acinetobacter (1 species), Brevibacterium (1 species), Pseudomonas (1 species), Agrococcus (1 species), Sanguibacter (1 species) and Virgibacillus (1 species). The haloversatile isolates were able to produce different enzymes such as catalase (100%), oxidase (80%), protease (52%), lecithinase (51%), cellulase (33%), lipase (23%), deoxyribonuclease (17%), pullulanase (10%), amylase (7%), urease (4%) and caseinase (4%). Scanning electron micrographs of sheep skins showed that catabolic activities of haloversatile bacterial species found in crude salt were responsible for decomposition of skins. When the crude salt containing haloversatile bacteria is used as a preservative for skins, these haloversatile bacteria may damage skin structure. Note de contenu : - Salt samples - Isolation of haloversatile bacteria from salt samples - Molecular identification of haloversatile isolates - Nucleotide accession number - Examination of cell morphology and pigmentation of haloversatile bacteria - Determining the effects of salt, pH and temperature on haloversatile bacterial growth - Examination of enzymatic activities of haloversatile bacteria - Sheepskin curing method and storage - Preparation of sheepskin samles for scanning electron microscope En ligne : https://drive.google.com/file/d/13mqUUdkonJ_bdMmp8izR5P9gUZXTJvtv/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33388 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
21427	-	Périodique	Bibliothèque principale	Documentaires	Disponible

Isolation and identification of moderately halophilic bacteria from soak liquor samples collected of leather tanneries / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVIII, N° 7 (07/2023)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVIII, N° 7 (07/2023) . - p. 293-300 Titre : Isolation and identification of moderately halophilic bacteria from soak liquor samples collected of leather tanneries Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur Année de publication : 2023 Article en page(s) : p. 293-300 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries -- Isolement Bactéries halophiles Bains de trempe -- Analyse Cuirs et peaux Cuirs et peaux -- Détérioration Cuirs et peaux de moutons Détérioration enzymatique Enzymes microbiennes Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Isolation and identification of protease and lipase producing moderately halophilic bacteria from soak liquor samples and studying their adverse effects to the sheepskin using scanning electron microscopy may provide critical data on decomposition of raw hide/skin materials during soaking process. Moreover, enzyme-production properties of the moderately halophilic isolates (such as catalase, oxidase, lipase, protease, urease, caseinase, amylase, cellulase, pullulanase, xylanase) were determined. The effects of different NaCl concentrations, pH and temperature values on the growth of moderately halophilic bacterial isolates were tested. In the present study, four moderately halophilic bacterial isolates were isolated and selected for further experiments. The isolated species designated as SLMHB5, SLMHB10, SLMHB12, SLMHB13 were similar to Vibrio alginolyticus, Terribacillus halophilus, Vibrio alginolyticus, and Vibrio parahaemolyticus species, respectively. Scanning electron micrographs of sheepskin samples demonstrated that enzymatic activities of moderately halophilic bacteria isolated from soak liquor samples which decomposed the skin structure. After 35-days storage period, the sheepskin sample showed bad odor, sticky appearance and hair slip. Hence, it is recommended to control these microorganisms during the soaking process with an effective antimicrobial agent. Note de contenu : - Soak liquor samples - Isolation of moderately halophilic bacteria from soak liquors - Molecular characterization of moderately halophilic isolates - GenGank accession number - Cell morphology and pigmentation of moderately halophilic isolates - Effects of NaCl, pH and temperature on moderately halohilic bacterial growth - Enzymatic activities of moderately halophilic isolates - Curing skins with enzyme producing moderately halophilic isolates and storage period - Preparation of stored skin samples for scanning electron microscope - Table 1 : Phenotypic characteristics of the moderately halophilic bacterial isolates DOI : https://doi.org/10.34314/jalca.v118i7.7857 En ligne : https://drive.google.com/file/d/1A42rj9bvYO_w8TZBnVg03im8stsdSJ3g/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39663 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
24133	-	Périodique	Bibliothèque principale	Documentaires	Disponible

Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents / Anne Lama in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVIII, N° 2 (02/2013)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CVIII, N° 2 (02/2013) Titre : Methods of isolation and identification of pathogenic and potential pathogenic bacteria from skins and tannery effluents Type de document : texte imprimé Auteurs : Anne Lama, Auteur ; Margaret Bates, Auteur ; Anthony D. Covington, Auteur ; Stuart C. Allen, Auteur ; A. Paula M. Antunes, Auteur Année de publication : 2013 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification Bactéries -- Isolement Bactéries pathogènes Cuirs et peaux -- Analyse Eaux usées -- Analyse Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Currently there is no standard protocol available within the leather industry to isolate and identify pathogenic bacteria from hides, skins or tannery effluent. This study was therefore carried out to identify simple but effective methods for isolation and identification of bacterial pathogens from the effluent and skins during leather processing. Identification methods based on both phenotypic and genotypic characteristics were investigated. Bacillus cereus and Pseudomonas aeruginosa were used as indicator bacteria to evaluate the isolation and identification methods. Decontaminated calfskins were inoculated with a pure culture of the above mentioned bacterial species followed by pre-tanning and chromium tanning processes. Effluent samples were collected and skins were swabbed at the end of each processing stage. Bacterial identification was carried out based on the phenotypic characteristics; such as colony appearance on selective solid media, cell morphology following a standard Gram-staining and spore staining techniques, and biochemical reactions, e.g., the ability of a bacterial species to ferment particular sugars and ability to produce certain enzymes. Additionally, an identification system based on bacterial phenotypic characteristics, known as Biolog® system was applied. A pulsed-filed gel electrophoresis (PFGE) method for bacterial DNA fingerprinting was also evaluated and used for the identification of the inoculated bacteria. The methods described in the study were found to be effective for the identification of pathogenic bacteria from skins and effluent. Note de contenu : - MATERIALS AND METHODS : Isolation and primary identification of inoculated bacterial species - Gram-staining and spore-staining - Biochemical following standard methods - The biolog system for bacterial identification - Extraction and purification of bacterial DNA for pulsed-field gel electrophoresis (Bacillus cereux - Pseudomonas aeruginosa) - Digestion of the bacterial DNA - Electrophoresis of bacterial DNA - RESULTS AND DISCUSSION : Bacterial growth on selective media (Bacillus cereus - Pseudomonas aeruginosa) - Gram-staining and spore-staining - Biochemical tests - The biolog system for bacterial identification - Electrophoresis of bacterial DNA En ligne : https://drive.google.com/file/d/1-cjGV1x0pnaNgB2dUiGLRtm5YRBYxREf/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=17372 [article]

Réservation

Réserver ce document

Exemplaires (1)

Code-barres	Cote	Support	Localisation	Section	Disponibilité
14610	-	Périodique	Bibliothèque principale	Documentaires	Disponible

---

1 (1 - 5 / 5)