[article]
| Titre : |
Actives protect epidermal stem cells against stresses |
| Type de document : |
texte imprimé |
| Auteurs : |
Laetitia Cattuzzato, Auteur ; Cindy Sanchez, Auteur ; Ambre de Pooter, Auteur ; Mickaël Puginier, Auteur ; Sandy Dumont, Auteur |
| Année de publication : |
2011 |
| Article en page(s) : |
p. 107-110 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Apoptose
Biomolécules actives
Cellules -- Cultures et milieux de culture
Cellules souches
Epiderme
Résistance au rayonnement ultraviolet
Stress oxydatif
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
Epidermal stem cells (ESC) are necessary for epidermis renewing. These cells are also known to be more or less resistant to different types of apoptosis, in comparison with their keratinocyte counterparts: UV-induced cell death,1 oxidative stress-induced apoptosis2 or anoïkosis (a particular apoptosis resulting from loss of adhesion to extracellular matrix).
Thus, some models have been developed to extract ESC from epidermis, to culture them and to investigate their specific functions such as colony-forming capacity or their sensitivity to oxidative stress.2 Survivin, an anti-apoptotic protein, has been shown to be specifically expressed in the nucleus of ESC, among an extracted population composed of ESC, transitoryamplifying cells and differentiated keratinocytes.4 ?Np63 is a p53 antiapoptotic homologue which has also been shown to be specifically expressed by ESC.5 It has been described to give UVB resistance to ESC and to decrease in these cells after such a stress.6 The NGF-pathway has also been shown to be implicated in this resistance, inducing an increase in the production of the antiapoptotic Bcl-2 molecule.1,7 Other ESCassociated markers have been described, such as MCSP (Melanoma Chondroitin Sulfate Proteoglycan)8,9 or a high level of ?1-integrin expression.3 The purpose of this study was, first, to develop new in vitro models to investigate ESC response after these different types of stresses and, second, to investigate the preventive effect of active ingredients. We particularly focused on cocoyl alanine (CA), an antioxidant and anti-wrinkle cosmetic product. Indeed, previously obtained results suggested that it was able to protect elderly skin explants from ex vivo culture-induced decrease in survivin expression (nuclear form) within epidermis. |
| Note de contenu : |
- METHODOLOGY : Reagents and materials - ESC extraction and culture - Investigation of ESC phenotype by immunofluorescence - Investigation of ESC resistance to UVB - Investigation of oxidative stress on ESC culture apoptosis - Investigation of UVB on ESC ΔNp63 level and apoptosis
- Investigation of AB1l on ESC anoïkosis - Image analysis, data analysis and statistical analysis
- RESULTS : Investigation of ESC phenotype - Investigation of ESC resistance to UVB - Investigation of H2O2 stress on ESC colony size and apoptosis - Investigation of UVB stress on ΔNp63 level - Investigation of the involvement of the NGF pathway in the UVB-induced apoptosis - Investigation of the effect of β1-integrin antibody on ESC anoikis |
| En ligne : |
https://drive.google.com/file/d/1i_5hLb_G--TulzuMImojOhc_OU0k0scp/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=12857 |
in PERSONAL CARE EUROPE > Vol. 4, N° 3 (09/2011) . - p. 107-110
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