Application of an enzymatic bate from local materials / M. M. Ahmed in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 87, N° 4 (07-08/2003)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 87, N° 4 (07-08/2003) . - p. 135-137 Titre : Application of an enzymatic bate from local materials Type de document : texte imprimé Auteurs : M. M. Ahmed, Auteur ; G. A. Gasmelseed, Auteur Année de publication : 2003 Article en page(s) : p. 135-137 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Confitage enzymatique Cuirs et peaux -- Propriétés chimiques Cuirs et peaux -- Propriétés physiques Cuirs et peaux de chèvres Cuirs et peaux de moutons Travail de rivière (cuir) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Over the years the enzymatic bating process for both hides and skins has become indispensable, it renders the leather soft, resilient and smooth. The basic raw materials for a bate have ranged from dog dung and pigeon faeces to synthetic enzymes. The application of the formerly used natural materials was difficult and the quality of the leather produced was not reproducible and was inconsistent. The skins might be over -bated or under -bated depending on the activity of the bate and the enzyme content of the material used. The current material used for the preparation of a bate is cow pancreas, blended with a carrier substance at a suitable concentration giving an activity of about 1300 units. The leather produced using such materials is compared with that produced using a widely applied commercial bating material (Oropon, Rohm and Haas) with regard to quality, strength and softness. Various compositions were used starting from 0.5% at increments of 0.25% to 1.5% based on limed weight. These trials were compared with a controlled experiment using 0.5% of the commercial bating material which has a strength of 1500 units as checked by the method of analysis adopted in this work (Appendix A). 72 wet-salted sheep and goat skins were divided equally into two groups, each group contained 6 skins of either type. The skins of the two groups were processed through soaking, unhairing, liming deliming, bating, pickling, chrome tannage, retannage, drying, and finishing. The leather produced is of good quality and it does not differ from the leather produced using imported bating materials. Note de contenu : - Producing the bate - Experimental work - Results and discussion : Summary of production - Application and results - Table 1 : Factors and their levels - Table 2 : Physical properties sheepskins - Table 3 : Physical properties of goat skins - Table 4 : Chemical properties of sheep skins En ligne : https://drive.google.com/file/d/1X0u0JoI8LOtbwsilfsGczdL9N62JX3XD/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39768 [article]

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Application of the extracellular proteases from the culture filtrate of streptomyces / S. Padmavathi in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 79, N° 3 (05-06/1995)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 79, N° 3 (05-06/1995) . - p. 88-90 Titre : Application of the extracellular proteases from the culture filtrate of streptomyces Type de document : texte imprimé Auteurs : S. Padmavathi, Auteur ; N. K. Chandrababu, Auteur ; S. Chitra, Auteur ; Gowri Chandrakasan, Auteur Année de publication : 1995 Article en page(s) : p. 88-90 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Confitage enzymatique Cuirs et peaux Enzymes microbiennes Essais (technologie) Evaluation visuelle Microscopie Peptidases Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Laboratory scale experiments were carried out to test the efficiency of the extracellular proteases from the culture filtrate of Streplomyces spp. G157 as a bate. A comparative study was carried out with the commercially available bate. Visual assessment, and microscopic analysis revealed that the extracellular proteases from the culture filtrate of Streptornyces spp. G157 were more efficient than the commercially available bate. Physical testing data indicated that it did not have any adverse effect on the properties of the leather. Note de contenu : - MATERIALS AND METHODS : Organism - Chemicals - Preparation of enzyme for evaluation of its bating action - Evaluation of enzyme for its bating action - Preparation of sample for Scanning Electron Microscopy - Physical Testing - RESULTS AND DISCUSSION : Visual assessment - Microscopic analysis - Physical testing data - Table 1 : Physical testing data En ligne : https://drive.google.com/file/d/141pz_e_zo3pzM1sTpqHnY9BtUej_HzSV/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40048 [article]

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Effect of electrostatic interaction between collagen and enzymes on permeation of protease into the pelt during leather bating process / Yiwen Zhu in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVIII, N° 10 (10/2023)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVIII, N° 10 (10/2023) . - p. 428-438 Titre : Effect of electrostatic interaction between collagen and enzymes on permeation of protease into the pelt during leather bating process Type de document : texte imprimé Auteurs : Yiwen Zhu, Auteur ; Jinzhi Song, Auteur ; Xu Zhang, Auteur ; Mengchu Gao, Auteur ; Biyu Peng, Auteur ; Chunxiao Zhang, Auteur Année de publication : 2023 Article en page(s) : p. 428-438 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Caractérisation Confitage enzymatique Cuirs et peaux Electrostatique Peptidases Perméation Poids Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The enzymatic delimed pelts bating process using proteases is critical to improving the overall performance of the leather. Bating effectiveness is determined not only by the properties but also by the permeation behavior of the proteases. Imperfect methods to control protease permeation often results in uneven distribution of enzyme proteins in the pelts, leading to excessive enzymolysis of the surface layer and inadequate opening-up of the inner layer. In this study, the relative size of proteases and delimed pelts were analyzed, the permeation behavior of fluorescein-labeled proteases in the pelt was observed using a confocal laser scanning microscope (CLSM), and the effect of electrostatic interaction between protease and collagen proteins on the permeation of protease into the pelt was investigated. The results showed that, after dehairing, liming and deliming operations, the enzyme can easily permeate into the pelts due to the formation of large cavities and interfibrillar gaps. The permeation of protease within the delimed pelt is significantly influenced by the affinity (electrostatic interactions) between the collagen and protease proteins. The isoelectric point (pI) of the protease protein, the collagen and the pH of the solution directly influence the electrostatic properties and interactions. When the enzyme and collagen are similarly charged (electrostatic repulsion), the enzyme can easily permeate into the pelts; when the enzyme and collagen are oppositely charged (electrostatic attraction), the permeation of the enzyme into the inner layer is difficult, resulting in the accumulation of protease on the grain and excessive hydrolysis of the grain layer. Therefore, the established permeation regulation mechanism of protease based on electrostatic interactions between enzyme and collagen could serve as an important basis for the selection of protease and the regulation of the enzymatic bating process. Note de contenu : - MATERIALS AND METHODS : Materials - Determination of protease particle size and delimed pelts pore diameter - Preparation and characteriztion of fluorescein-labeled proteases - RESULTS AND DISCUSSION : The particle size of protease in solution and pore diameter of delimed pelts - Determination of the pI of the delimed pelt - Characterization of the FITC-labeled proteases - UV-Vis spectroscopy characterization of FITC-labeled proteases - FLuorescence spectroscopy characterizations of FITR labeled proteases - pI and relative molecular weight of labeled proteases - Effect of proteins affinity on proteases permeation behavior in delimed pelts - Effect of pH on the surface of the pelts during enzymatic bating - Charge regulation mechanism of protease permeation in delimed pelts DOI : https://doi.org/10.34314/jalca.v118i10.8231 En ligne : https://drive.google.com/file/d/1xABUCLjci5t-d9y12Zp6saB4Vg83zurc/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39941 [article]

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Hide powder azure and azocoll as substrates for assay of the proteolytic activity of bate / Samuel M. Mozersky in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. LXXXVII, N° 8 (08/1992)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXVII, N° 8 (08/1992) . - p. 287-295 Titre : Hide powder azure and azocoll as substrates for assay of the proteolytic activity of bate Type de document : texte imprimé Auteurs : Samuel M. Mozersky, Auteur ; David G. Bailey, Auteur Année de publication : 1992 Article en page(s) : p. 287-295 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Collagène Confitage enzymatique Enzymes pancréatiques Poudre de peaux Protéolyse En biochimie, la protéolyse est la segmentation des protéines en ses fragments de base (acides aminés) via l'hydrolyse catalysée par des enzymes dits "protéolytiques" (protéases ou hydrolases). (Wikipedia) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Hide powder azure (HPA) and azocoll (Az) were evaluated for their suitability as collagen-rich substitutes for casein in assaying for the proteolytic activity of bate. Optimum conditions were established and a recommended procedure is presented. Under these conditions, the proteolytic activity of bate can be evaluated using an incubation period of one minute. Commercially available HPA and Az of small particle size were both found to be suitable substrates, provided the prescribed conditions are carefully observed. Evaluation of a commercially available preparation of coarse Az indicated that it is less suitable as a substrate for the assay. Note de contenu : - Incubation buffer - Agitation - Termination of enzyme action - Washing the substrate - Time course of proteolysis - Influence of the amount of bate - Pancreatic bate - Precision - Recommended procedure - Table 1 : Precision of measurement of reaction velocity En ligne : https://drive.google.com/file/d/1kFYIu78JLplzs6CVejKWxTa2W9jwWtAw/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39841 [article]

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