Bright fluorescent dsDNA probes : novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine-quinolinium chromophore / Aleksey Vasilev in COLORATION TECHNOLOGY, Vol. 131, N° 2 (04/2015)  

[article]  inCOLORATION TECHNOLOGY > Vol. 131, N° 2 (04/2015) . - p. 94-103 Titre : Bright fluorescent dsDNA probes : novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine-quinolinium chromophore Type de document : texte imprimé Auteurs : Aleksey Vasilev, Auteur ; Nedyalko Lesev, Auteur ; Sonya Dimitrova, Auteur ; Marina Nedelcheva-Veleva, Auteur ; Stoyno Stoynov, Auteur ; Silvia Angelova, Auteur Année de publication : 2015 Article en page(s) : p. 94-103 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : ADN Analyse spectrale Colorants Colorants -- Synthèse Diagnostic biologique Electrophorèse Fluorescence Index. décimale : 667.3 Teinture et impression des tissus Résumé : Seven tri- and tetracationic monomeric and homodimeric monomethine cyanine dyes based on thiazolo[4,5-b]pyridinium and quinolinium end groups were synthesised and characterised. The dyes were tested as fluorescent DNA intercalating probes to apply in DNA gel electrophoresis. The DNA samples stained with all dyes from the series demonstrated bright fluorescent signals. DNA fragments were successfully visualised under orange and green filters as well as under standard UV transillumination. Two of the studied dyes revealed higher sensitivity to DNA when compared with the commercial dimeric cyanine dye TOTO-1. Their sensitivity reached that of the commercial dimeric cyanine dye YOYO-1, but the emission was shifted to longer wavelengths. These qualities make the dyes suitable to apply in a wide range of medical and scientific analytical methods. Note de contenu : - EXPERIMENTAL : Materials - Equipment - Synthesis of dye 3 - Synthesis of tri- and tetracationic dyes 5a to 5g - Preparation of dyes and DNA solutions - Staining after electrophoresis - Staining prior to electrophoresis - RESULTS AND DISCUSSION : Synthesis and structural characterisation of dyes 5a to 5g - Spectroscopic characteristics of the dyes and computational study of the chromophore before binding to dsDNA - Spectroscopic characteristics after binding to dsDNA - Staining after electrophoresis completion - Staining DNA samples prior to electrophoresis - Retardation of the mobility of stained DNA fragments DOI : 10.1111/cote.12133 En ligne : https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12133 Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=23656 [article]

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Novel environmentally benign procedure for the synthesis of 2-aryl- and 2-hetaryl-4(3H)-quinazolinones / Todor Deligeorgiev in COLORATION TECHNOLOGY, Vol. 126, N° 1 (2010)  

[article]  inCOLORATION TECHNOLOGY > Vol. 126, N° 1 (2010) . - p. 24-30 Titre : Novel environmentally benign procedure for the synthesis of 2-aryl- and 2-hetaryl-4(3H)-quinazolinones Type de document : texte imprimé Auteurs : Todor Deligeorgiev, Auteur ; Ana M. Cuadro, Auteur ; Julio Alvarez-Buila, Auteur ; Juan J. Vaquero, Auteur ; Aleksey Vasilev, Auteur ; Stefka Kaloyanova, Auteur Année de publication : 2010 Article en page(s) : p. 24-30 Note générale : Bibliogr. Langues : Anglais (eng) Index. décimale : 667.3 Teinture et impression des tissus Résumé : A novel environmentally benign procedure for the synthesis of 2-aryl- or 2-hetaryl-4(3H)-quinazolinones by condensation of anthranilamide with various aromatic aldehydes in polyethylene glycol under microwave irradiation has been developed. In contrast with other reported methods, this procedure provides the corresponding quinazolinones with good to high yields and purity, in very short reaction times and without the use of an oxidant. DOI : 10.1111/j.1478-4408.2010.00223.x En ligne : http://onlinelibrary.wiley.com/doi/10.1111/j.1478-4408.2010.00223.x/pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=8414 [article]

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A novel general method for fast and easy preparation of cationic, neutral dimethinehemicyanine and dimethine dyes by uncatalysed Knoevenagel condensation / Todor Deligeorgiev in COLORATION TECHNOLOGY, Vol. 128, N° 6 (2012)  

[article]  inCOLORATION TECHNOLOGY > Vol. 128, N° 6 (2012) . - p. 417-424 Titre : A novel general method for fast and easy preparation of cationic, neutral dimethinehemicyanine and dimethine dyes by uncatalysed Knoevenagel condensation Type de document : texte imprimé Auteurs : Todor Deligeorgiev, Auteur ; Nikolay Gadjev, Auteur ; Stefka Kaloyanova, Auteur ; Nedyalko Lesev, Auteur ; Aleksey Vasilev, Auteur ; Alexi Alexiev, Auteur Année de publication : 2012 Article en page(s) : p. 417-424 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Absorption Colorants -- Synthèse Colorants cationiques Fluorescence Index. décimale : 667.3 Teinture et impression des tissus Résumé : Three novel general experimental procedures for preparation of cationic, neutral dimethinehemicyanine and dimethine dyes (eight of which are unpublished) by uncatalysed Knoevenagel condensation have been developed. The proposed methods are versatile as a variety of different starting materials can be used and they ensure good to very high yields, very short reaction times and high purity of the final dyes. Absorption and fluorescent properties of the novel dyes were investigated. DOI : 10.1111/j.1478-4408.2012.00408.x En ligne : http://onlinelibrary.wiley.com/doi/10.1111/j.1478-4408.2012.00408.x/pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=16442 [article]

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Styryl dyes - synthesis and applications during the last 15 years / Todor Deligeorgiev in COLORATION TECHNOLOGY, Vol. 126, N° 2 (2010)

[article]  inCOLORATION TECHNOLOGY > Vol. 126, N° 2 (2010) . - p. 55-80 Titre : Styryl dyes - synthesis and applications during the last 15 years Type de document : texte imprimé Auteurs : Todor Deligeorgiev, Auteur ; Aleksey Vasilev, Auteur ; Stefka Kaloyanova, Auteur ; Juan J. Vaquero, Auteur Année de publication : 2010 Article en page(s) : p. 55-80 Note générale : Bibliogr. Langues : Anglais (eng) Index. décimale : 667.3 Teinture et impression des tissus Résumé : One of the most widely used and important groups of functional dyes are the styryl dyes and a review of this functional dye class has not been published for more than 15 years. In this review article, we describe the new trends in the synthesis of a range of novel intermediates and styryl dyes and include the most interesting examples of their high-tech applications. However, this review is not intended to be comprehensive because of the large number of styryl dye studies that have been carried out in this time. Styryl cyanine dyes are widely used in optical recording media in laser discs, as flexible dyes, laser dyes, as optical sensitisers and in various other fields, for example dye-sensitised solar cells and dyes with non-linear optical properties. Additionally, the most important applications for these dyes are in bio-labelling and in medicinal analysis. DOI : 10.1111/j.1478-4408.2010.00235.x Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=9278 [article]

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Synthesis of novel tetracationic asymmetric monomeric monomethine cyanine dyes – highly fluorescent dsDNA probes / Aleksey Vasilev in COLORATION TECHNOLOGY, Vol. 127, N° 1 (2011)  

[article]  inCOLORATION TECHNOLOGY > Vol. 127, N° 1 (2011) . - p. 69-74 Titre : Synthesis of novel tetracationic asymmetric monomeric monomethine cyanine dyes – highly fluorescent dsDNA probes Type de document : texte imprimé Auteurs : Aleksey Vasilev, Auteur ; Todor Deligeorgiev, Auteur ; Stefka Kaloyanova, Auteur ; Stanimir Stoyanov, Auteur ; Vera Maximova, Auteur ; Juan J. Vaquero, Auteur ; Julio Alvarez-Buila, Auteur Année de publication : 2011 Article en page(s) : p. 69-74 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : ADN Cyanines monométhines Fluorescence Lumière -- Absorption Pigments à effets spéciaux -- Effets de la lumière Index. décimale : 667.3 Teinture et impression des tissus Résumé : Quaternisation of monomethine cyanine dyes bearing haloalkyl chains, with the appropriate monoquaternised 1,4-diazabicyclo[2.2.2]octane derivatives, yielded novel tetracationic asymmetric monomeric monomethine cyanine dyes belonging to the thiazole orange family. The optimal conditions for this reaction were investigated. The longest wavelength absorption maxima of the studied dyes are in the region 504–507 nm. The molar absorptivities of the target dyes are high, with values between 70 500 and 99 500 l mol?1 cm?1. The fluorescence intensity and the fluorescence quantum yields of the free dyes in solution or in the presence of double-stranded DNA were determined using thiazole orange for comparison. The dyes are suitable for nucleic acid detection and have a high binding affinity, attributable to their four positive charges. DOI : 10.1111/j.1478-4408.2010.00280.x En ligne : http://onlinelibrary.wiley.com/doi/10.1111/j.1478-4408.2010.00280.x/pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10906 [article]

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