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Reduction of DNA damage by nicotinamide after reactive oxygen species treatment / S. C. Chen in IFSCC MAGAZINE, Vol. 5, N° 1 (01-02-03/2002)
[article]
Titre : Reduction of DNA damage by nicotinamide after reactive oxygen species treatment Type de document : texte imprimé Auteurs : S. C. Chen, Auteur ; Y. Takasli, Auteur ; Y.-C. Chen, Auteur ; T. H. Tsai, Auteur ; C. H. Hu, Auteur ; W. R. Lee, Auteur Année de publication : 2002 Article en page(s) : p. 3-6 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : ADN -- Réparation
Apoptose
Nicotinamide
Peroxyde d'hydrogèneLe peroxyde d'hydrogène (H2O2), communément appelé eau oxygénée ou encore perhydrol (appellation industrielle), est un composé chimique liquide et visqueux, aux puissantes propriétés oxydantes (il est aussi réducteur). C'est donc un agent blanchissant efficace qui sert de désinfectant et (à haute concentration) d'oxydant ou monergol dans les fusées spatiales.
Rayonnement ultravioletTags : Nicotinamide 'Ultraviolet C' (UVC) 'OPeroxyde d'hydrogène' (ROS)' 'Dommage de l'ADN'' Apoptose '8-hydroxy-2'-deoxyguanosine(8-OhdG)' Index. décimale : 668.5 Parfums et cosmétiques Résumé : We investigated whether nicotinamide acts as a preventive agent of apoptosis induced by H2O2 and UVC irradiation in skin cells including keratinocyte HaCat and NIH3T3 cells. In this study, both H2O2 (450μM) and UVC (40J/m2) irradiation-induced cell death were detected by MTT assay, and nicotinamide showed the dose dependent inhibition on H2O2 and UVC induced cell death. Morphological study by microscopic observation showed that H2O2 or UVC induced cellular morphological changes including appearence of rounded cells or swelling cells, characteristic og apoptosis. In the presence of nicotinamide, H2O2 and UVC induced morphological changes were attenuated significantly. Prevention of cells from H2O2 and UVC induced cell death was asociated with decrease of apoptotic bodies by flow cytometry analysis and attenuation of the appearance of DNA ladders by DNA electrophoresis. Induction of p53 protein was detected in H2O2-treated NIH3T3 cells and nicotinamide suppressed its increase. However, bcl-2, bax and α-tubulin proteins were remained unchanged. ROS have been demonstrated to be involved in the H2O2 and UVC induced apoptosis. In this study, H2O2 or UVC irradiation increase intracellular peroxide levels detected by DCHF-DA assay and fluorescent microscopic observation, and nicotinamide blocked the peroxide level induced by H2O2 or UVC irradiation. Decrease of H2O2 or UVC-induced 8-OH dG in DNA was also detected in nicotinamide trated cells. The results of present study firstly demonstrated that nicotinamide prevented cells from H2O2 and UVC irradiation induced apoptosis through suppression ROS production, p53 elevation and 8-OH dG formation. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10656
in IFSCC MAGAZINE > Vol. 5, N° 1 (01-02-03/2002) . - p. 3-6[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 004652 - Périodique Bibliothèque principale Documentaires Disponible Les régulations endocriniennes de la peau in PARFUMS COSMETIQUES ACTUALITES, N° 132 (12/96 - 01/1997)
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Titre : Les régulations endocriniennes de la peau Type de document : texte imprimé Année de publication : 1996 Article en page(s) : p. 53-54 Langues : Français (fre) Tags : 'Régulation hormonale' Peau Biosynthèse Apoptose 'Article synthèse' Homme Index. décimale : 668.5 Parfums et cosmétiques Résumé : Pour étudier l'évolution des processus biologiques notamment endocriniens, on peut choisir des modèles animaux qui présentent des caractéristiques particulières de par leur position phylogénétique. C'est ainsi que l'on a pu découvrir des analogies entre le fonctionnement du tissu osseux et celui de la peau. Note de contenu : - La biominéralisation
- Les hormones synthétisées par la peau
- Apoptose et peau
- BAX et BCL2Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=19430
in PARFUMS COSMETIQUES ACTUALITES > N° 132 (12/96 - 01/1997) . - p. 53-54[article]Exemplaires (1)
Code-barres Cote Support Localisation Section Disponibilité 002603 - Périodique Bibliothèque principale Documentaires Exclu du prêt Differential toxicity on monocytes and monocyte-derived dendritic cells : a new tool to differentiate allergens from irritants ? / Laetitia Furio in IFSCC MAGAZINE, Vol. 10, N° 1 (01-02/2007)
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Titre : Differential toxicity on monocytes and monocyte-derived dendritic cells : a new tool to differentiate allergens from irritants ? Type de document : texte imprimé Auteurs : Laetitia Furio, Auteur ; J. Guesnet, Auteur ; Blandine Ducarre, Auteur ; A. Guezennec, Auteur ; Daniel Schmitt, Auteur ; josette Peguet-Navarro, Auteur Année de publication : 2007 Article en page(s) : p. 27-33 Note générale : Bibliogr. Langues : Anglais (eng) Tags : 'Hypersensibilité cutanée' 'Méthode alternative in-vitro' 'Cellules dendritiques' Monocytes Apoptose Index. décimale : 668.5 Parfums et cosmétiques Résumé : Phenotypic activation of monocyte-derived dendritic cells has been proposed as an in vitro alternative assay to discriminate potential sensitizers from irritants, but the sensitivity of the assay remains controversial. In this study, we first determined the dynamic range of expression of activation/maturation markers on human monocyte-derived dendritic cells cultured in the presence or absence of transforming growth factor ß (TGFβ)? On day three of culture, most monocytes had already differentiated into dendritic cells that expressed low levels of costimulatory molecules especially in the presence TGFβ-treatment of 3-day-old TGFβ-treated monocyte-derived dendritic cells with several chemicals at sub-toxic concentrations induced significant phenotypic changes for all the strong and mild sensitizers tested, whereas the irritant sodium lauryl sulfate had no effect. However, a very large variability was observed among the experiments. Most interestingly, we could show here for the first time that at concentrations sub-toxic for monocyte-derived dendritic cells all the allergens tested induced monocyte apoptosis within 2 days of culture. In contrast, sodium lauryl sulfate displayed similar toxicity on monocytes and monocyte-derived dendritic cells and these results were confirmed with other irritants such as benzoic acid or methylsalicylate. Although testing of far more chemicals is required, these results indicate that differential toxicity of chemicals to monocytes and monocyte-derived dendritic cells could be a rapid, simple and valuable tool to differentiate sensitizers from irritants. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10425
in IFSCC MAGAZINE > Vol. 10, N° 1 (01-02/2007) . - p. 27-33[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 007646 - Périodique Bibliothèque principale Documentaires Disponible