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Characterization and control of antibiotic-resistant calmati saltern's isolates with bacteriocins / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVII, N° 8 (08/2022)
[article]
Titre : Characterization and control of antibiotic-resistant calmati saltern's isolates with bacteriocins Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur Année de publication : 2022 Article en page(s) : p. 315-329 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification
Bactéries -- Isolement
Bactéries halophiles
BactériocinesLes bactériocines sont une famille de peptides ou protéines synthétisés naturellement par certaines bactéries. Une bactériocine consiste généralement en un composé protéique de 20 à 60 acides aminés.
Les bactériocines ne sont pas des antibiotiques mais elles possèdent des propriétés antibiotiques :
- Elles peuvent être bactériocides, c'est-à -dire éliminer certains micro-organismes.
- Elles peuvent être bactériostatiques, c'est-à -dire inhiber la croissance de certains micro-organismes.
Caractérisation
Concentration bactéricide minimaleLa concentration bactéricide minimale (CBM) est la plus faible concentration d'un agent antibactérien nécessaire pour tuer une bactérie particulière1. Elle peut être déterminée à partir des tests de concentration minimale inhibitrice (CMI) de dilution en bouillon en repiquant les bactéries sur des plaques d'agar qui ne contiennent pas l'agent antibactérien que l'on teste. La CBM est identifiée en déterminant la concentration la plus faible d'agent antibactérien qui réduit la viabilité de l'inoculum bactérien initial à un niveau supérieur ou égal à 99,9%. La CBM est complémentaire à la CMI ; alors que le test CMI indique le niveau le plus bas d'agent antimicrobien qui inhibe la croissance, le CBM indique le niveau le plus bas d'agent antimicrobien qui entraîne la mort microbienne. Cela signifie que même si une CMI particulière montre une inhibition, le repiquage des bactéries sur de la gélose peut encore entraîner la prolifération de l'organisme car l'antimicrobien n'a pas causé la mort. Les agents antibactériens sont généralement considérés comme bactéricides si la CBM ne dépasse pas quatre fois la CMI. Comme le test CBM utilise des unités formant colonies comme mesure indirecte de la viabilité bactérienne, il peut être biaisé par des agents antibactériens qui provoquent l'agrégation des cellules bactériennes. On peut citer en exemples les flavonoïdes et les peptides. (Wikipedia)
Concentration minimale inhibitrice (antimicrobiens)En microbiologie, la concentration minimale inhibitrice (CMI) est la plus faible concentration d'un produit chimique, généralement un médicament, qui empêche la croissance visible d'une ou de plusieurs bactéries. La CMI dépend du micro-organisme considéré, de l'être humain affecté (in vivo uniquement) et de l'antibiotique lui-même.
La CMI est déterminée en préparant des solutions du produit chimique in vitro à diverses concentrations croissantes, en incubant les solutions avec des groupes séparés de bactéries en culture et en mesurant les résultats en utilisant une méthode de dilution standardisée (agar ou microdilution). Les résultats se classent ensuite comme "sensible", "intermédiaire" ou "résistant" à un antimicrobien particulier en utilisant un point d'arrêt. Les points d'arrêt sont des valeurs convenues, publiées dans les directives d'un organisme de référence, comme le US Clinical and Laboratory Standards Institute (CLSI), la British Society for Antimicrobial Chemotherapy (BSAC) ou le Comité européen sur les tests de sensibilité aux antimicrobiens (EUCAST). On a pu constater des écarts importants au niveau des points d'arrêt de divers pays européens au fil des ans, et entre ceux de l'EUCAST et du CLSI.
Alors que la CMI est la concentration la plus faible d'un agent antibactérien nécessaire pour inhiber la croissance visible, la concentration bactéricide minimale (CBM) est la concentration minimale d'un agent antibactérien qui entraîne la mort bactérienne. Plus la CMI est proche de la CBM, plus le composé est bactéricide.
La première étape de la découverte d'un médicament est souvent le dépistage d'un médicament candidat de banque de données pour les CMI contre les bactéries d'intérêt. En tant que tels, les CMI sont généralement le point de départ pour de plus grandes évaluations précliniques de nouveaux agents antimicrobiens. Le but de la mesure de la concentration minimale inhibitrice est de s'assurer que les antibiotiques sont choisis efficacement pour augmenter le succès du traitement. (Wikipedia)
Microbiologie
Résistances aux antibiotiques
Sels de sodiumIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Camalti Saltern is the largest solar saltern in Izmir, Turkey. The salt obtained from Camalti Saltern is used in food and leather industries to prevent bacterial growth. In this saltern, seawater is pumped into shallow ponds. Then, the sun and wind cause evaporation and crystallization, finally sea salt is produced. Due to the fact that Camalti Saltern contains various halophilic bacteria, the goals of the present study were to isolate and identify haloversatile bacteria from Camalti Saltern’s brine samples, to examine their antibiotic resistance profiles, to determine antimicrobial activities under optimum environmental conditions, to determine bacteriocin concentration by Bradford Method, to detect Minimum Inhibitory Concentrations (MIC) and Minimum Bactericidal Concentrations (MBC) of bacteriocins against multidrug-resistant isolates, and to observe the cell structure of bacteriocin-treated bacteria under SEM. Sixteen bacterial isolates were recovered from Camalti Saltern’s brine samples and were identified as 14 different species (Bacillus haynesii, Bacillus simplex, Bacillus subtilis subsp. stercoris, Bacillus pumilus,Staphylococcus petrasii subsp. jettensis, Staphylococcus saprophyticus subsp. saprophyticus, Kocuria sediminis, Rhodococcus enclensis, Marinobacter hydrocarbonoclasticus, Vibrio olivae, Marinomonas communis, Pseudomonas psychrotolerans, Salinivibrio costicola subsp. vallismortis, Vibrio neocaledonicus). Percentages of antibiotic resistance of isolates were 63% to aztreonam, 50% to amoxicillin/clavulanic acid, 44% to ampicillin, 44% to cefadroxil, 31% to imipenem, 19% to ampicillin/sulbactam, 6% to chloramphenicol, 6% to tetracycline, 6% to mupirocin, 6% to meropenem. The bacteriocin concentrations of Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis were measured as 1.02 mg/mL and 1.25 mg/mL, respectively. Bacteriocins of Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis, which were not resistant to any antibiotics tested,exhibited the inhibitory effect against Kocuria sediminis resistant to ten antibiotics and Bacillus pumilus resistant to four antibiotics. Bacteriocin of Salinivibrio costicola subsp. vallismortis also demonstrated the inhibitory effect against Pseudomonas psychrotolerans resistant to five antibiotics. Scanning electron micrographs showed that cell morphologies of bacteriocin-treated isolates (Kocuria sediminis, Bacillus pumilus, Pseudomonas psychrotolerans) were damaged. In conclusion, bacteriocins produced from the haloversatile Camalti saltern isolates may be used in the leather industry to prevent the growth of antibiotic-resistant haloversatile bacteria. Note de contenu : - Sample collection
- Isolation of bacteria
- Characterization of the isolates
- Amplification and sequencing of 16S rRNA genes
- Nucleotide accession number
- Antibiotic susceptibility
- Preparation of cell-free supernatant
- Screening of antimicrobial activity among the test isolates
- Determination of protein concentration using Bradford method
- Optimum conditions for bacteriocin production from Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis
- Effect of heat on bacteriocins produced by Rhodococcus enclensis and Salnivibrio costicola subsp. vallismortis
- Effect of pH on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis
- Effect of NaCl concentration on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis
- Effect of enzymes (Proteinase K and Lipase) on bacteriocins produced by Rhodococcus enclensis and Salinivibrio costicola subsp. vallismortis
- Minimum inhibitory concentrations and minimum bactericidal concentrations of bacteriocins against multidrug-resistant isolates
- Preparation of bacteriocin-treated and bacteriocin-untreated cells for scanning electron microscopy
- Table 1 : Characteristics of haloversatile bacteria isolated from brine samples of the Camalti Saltern
- Table 2 : Inhibition zone diameter measurements (mm) of haloversatile bacteria isolated from brine samples of the Camalti Saltern
- Table 3 : Inhibitory effect of bacteriocin produced by bacterial isolates against each other
- Table 4 : Inhibition zone diameters (mm) of multidrug-resistant haloversatile bacteria against bacteriocins obtained from Rhodococcus enclensis, Salinivibrio Costicola subsp. vallismortis at different incubation temperatures, pH and NaC1 concentrations
- Table 5 : The effects of different temperatures, pH, NaC1 concentrations, proteinase K and lipase enzymes on antibacterial effect of bacteriocins
- Table 6 : The Minimum Inhibitory Concentrations of bacteriocin produced from Rhodococcus enclensis against Kocuria sediminis and Bacillus pumilus
- Table 7 : The Minimum Inhibitory Concentrations of bacteriocin produced from Salinivibrio costicola subsp. vallismortis against Kocuria sediminis, Bacillus pumilus and Pseudomonas psychrotoleransDOI : https://doi.org/10.34314/jalca.v117i8.5982 En ligne : https://drive.google.com/file/d/1G3HZoIp4GH7W3A22JxMjjbmRimXH0dkk/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37824
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Code-barres Cote Support Localisation Section Disponibilité 23516 - Périodique Bibliothèque principale Documentaires Disponible Characterization and corrosion protection properties of composite material (PANI+TiO2) coatings on A304 stainless steel / Souhila Abaci in JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH, Vol. 12, N° 1 (01/2015)
[article]
Titre : Characterization and corrosion protection properties of composite material (PANI+TiO2) coatings on A304 stainless steel Type de document : texte imprimé Auteurs : Souhila Abaci, Auteur ; Belkacem Nessark, Auteur Année de publication : 2015 Article en page(s) : p. 107-120 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Acier inoxydable
AcierL'acier est un alliage métallique utilisé dans les domaines de la construction métallique et de la construction mécanique.
L'acier est constitué d'au moins deux éléments, le fer, très majoritaire, et le carbone, dans des proportions comprises entre 0,02 % et 2 % en masse1.
C'est essentiellement la teneur en carbone qui confère à l'alliage les propriétés du métal qu'on appelle "acier". Il existe d’autres métaux à base de fer qui ne sont pas des aciers comme les fontes et les ferronickels par exemple.
Anticorrosifs
Anticorrosion
Caractérisation
Dioxyde de titane
Métaux -- Revêtements protecteurs
pH
Polyaniline
Résistance chimiqueIndex. décimale : 667.9 Revêtements et enduits Résumé : This work presents the corrosion protection behavior of A304 stainless steel in an acidic medium by using coatings based on polyaniline+TiO2 composite material. The influence of parameters such as concentration of aniline, TiO2 content, and pH of the solution were investigated. The coatings which had been deposited by cyclic voltammetry on substrates of A304 steel were then characterized by electrochemical impedance spectroscopy. The cyclic voltammograms showed three redox couples characteristic of the different oxidation and reduction states of the produced polymer. PANI+TiO2 composite material was observed to exhibit higher corrosion resistance and better properties. The effectiveness of coatings in preventing corrosion was tested by potentiodynamic polarization studies and scanning electron microscopy was used to characterize the morphology of the coatings. The results showed that PANI+TiO2 coatings offer good anticorrosion protection to steel in 1 M H2SO4 solutions. The micrograph taken at the coatings surface showed that (PANI+TiO2)/A304 composite was uniform in nature and TiO2 particles were uniformly covered by PANI. After immersion into a corrosive solution for 45 min, no aggressive effect was observed and the coating films were still present. Moreover, the formation of PANI+TiO2 composite was also confirmed by EDX. Furthermore, it was found that the presence of a low amount of TiO2 in PANI coatings afforded the best protection due to the formation of a coating layer on the metallic surface which behaved like a physical barrier against the aggressive medium attack. Note de contenu : - EXPERIMENTAL : Materials - Methods and electrodeposition of PANI+TiO2 composite film - Characterization techniques
- RESULTS AND DISCUSSION : Electropolymerization of aniline in H2SO4 environment on an A304 stainless steel electrode - Effect of TiO2 content in the film deposited on 1304 stainless steel - Effect of the aniline concentration on stainless steel - Protection of the stainless steel by the polyaniline modified by the incorporation of TiO2 (Tafel polarization curves) - Study by impedance spectroscopy of the electrode modified (PANI+TiO2)/A304 - Morphological characterizaton of the steel, PANI, and composite films by SEM - Chemical analysis of the composites by EDXDOI : 10.1007/s11998-014-9611-x En ligne : https://link.springer.com/content/pdf/10.1007%2Fs11998-014-9611-x.pdf Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=23211
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Code-barres Cote Support Localisation Section Disponibilité 16952 - Périodique Bibliothèque principale Documentaires Disponible Characterization and evaluation of fungal enzymatic pool with unhairing activity / B. C. Galarza in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 100, N° 5 (09-10/2016)
[article]
Titre : Characterization and evaluation of fungal enzymatic pool with unhairing activity Type de document : texte imprimé Auteurs : B. C. Galarza, Auteur ; M. Garro, Auteur ; J. Martegani, Auteur ; R. Hours, Auteur Année de publication : 2016 Article en page(s) : p. 257-262 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Caractérisation
Cuirs et peaux -- Analyse
Cuirs et peaux de bovins
Enzymes fongiques
Enzymes protéolytiquesUne enzyme protéolytique est une enzyme capable de couper une protéine en plusieurs fragments ou peptides. La trypsine, la papaïne, la pepsine, la chymotrypsine, la plasmine, la subtilisine... sont capables de couper une protéine, chaque enzyme étant spécifique de certains sites particuliers de cette protéine. C'est ainsi, par exemple, qu'une immunoglobuline G est découpée par la papaïne en un fragment Fc et deux fragments Fab, comme l'a montré Porter en 1959.
Epilage enzymatique
Microscopie électronique à balayageIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : The use of enzymes in the leather industry in the beamhouse area to partially replace sulfide, can reduce to almost 50% the H2S emission. In this enzyme treatment proteolysis needs to be controlled to prevent the extension of activity to collagen. Collagen must keep its optimal conditions for the tannage process and to give high quality leather. In this sense it is necessary to characterize the enzyme from the physicochemical point of view and to study the morphological changes produced in bovine skin to control its action. An enzymatic pool of geophilic strain Trichophyton ajelloi cultured in solid media was characterized. Azocaseinolytic and keratinolytic activity was determined under different conditions. The enzymatic pool showed tolerance to high T°, optimal pH9 in bath substrates, partial inhibition in concentrations 0.1M, 0.01M and 0.001 M from metallic salts (Ca Cl2, BaCl2, KCl, CuSO4), activation by NaCl, reducing agents as Na2S03, Na2S, L-cystein hydrochloride monohydrate, thioglycolic acid and commercial anionic and non-ionic surfactants (SDS, isogràs AN, Triton, Baymol, Azymol). Inactivation exerted by. PMSF showed a serine-protease as a component of the pool (MEROPS system). When the enzymatic pool was applied for 24-48 hours under optimal conditions on bovine skin pieces, a depilatory affect was observed. The changes in the bovine skin morphology were observed by SEM, alter fixation in formaldehyde and dehydration with alcohol as part of a tentative protocol for bovine skin analysis by SEM (Scanning Electron Microscopy). Note de contenu : - Solid-state culture
- Characterization of crude extract
- Test procedures
- SEM : sample preparation and observationEn ligne : https://drive.google.com/file/d/1Cn1wPo2dkSvZQZ62tGJGbpyJQQ1Ylr7Q/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27091
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Code-barres Cote Support Localisation Section Disponibilité 18411 - Périodique Bibliothèque principale Documentaires Disponible Characterization and mechanism of zinc salts as tanning agents / Shan Cao in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CVIII, N° 11 (11/2013)
[article]
Titre : Characterization and mechanism of zinc salts as tanning agents Type de document : texte imprimé Auteurs : Shan Cao, Auteur ; Baozhen Cheng, Auteur ; Qiaoe Wang, Auteur ; Bing Liu, Auteur Année de publication : 2013 Article en page(s) : p. 428-433 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Agents de tannage
Caractérisation
Cuirs et peaux
Sels métalliques
ZincIndex. décimale : 675.2 Préparation du cuir naturel. Tannage Résumé : Zinc has important biological functions as one of the essential trace elements to human. The Zinc tanning agent could produce white leathers with properties comparable to the chrome tanning agent, and the tanning agents based on Zinc salts were prepared using different masking agents such as sodium formate, sodium acetate, sodium tartrate and potassium hydrogen phthalate. The selection of the zinc tanning agents has been optimized based on the properties like hydrothermal stability and percentage exhaustion of zinc. The results indicated that zinc sulfate can be used as tanning agent under acidic conditions (50g/L, pH=3, 25°C) and is an effective and superior method. Moreover, Sodium citrate (0.1mol/L) was selected as masking agent compared with other masking agents. The hydrothermal stability was improved effectively with the action of masking agent. Note de contenu : - EXPERIMENTAL PROCEDURES : Equipment - Materials - Preparation of zinc ion measuring reagent - Preparation of tanning reagent - The optimal conditions of zinc tanning procedure - The selection of the masking agent for zinc tanning
- RESULTS AND DISCUSSION : Hydrothermal stability of wet leather tanned with zinc salts - The effects of masking agent - The principle of zinc tanning
- Table I : Type and concentration of zinc tanning agent
- Table II : The hydrothermal stability of different concentration of zinc tanning agent
- Table III : The selection of the zinc agent's species
- Table IV : Hydrothermal stability the masking agent's species in zinc tanning processEn ligne : https://drive.google.com/file/d/1l3UkjsTmwceI2XZXciXSUZCOia90qlzY/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=19691
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Code-barres Cote Support Localisation Section Disponibilité 15707 - Périodique Bibliothèque principale Documentaires Disponible Characterization and properties of gradient polyacrylate latex particles by gradient emulsion polymerization / Xinya Zhang in JOURNAL OF COATINGS TECHNOLOGY AND RESEARCH, Vol. 9, N° 6 (11/2012)
[article]
Titre : Characterization and properties of gradient polyacrylate latex particles by gradient emulsion polymerization Type de document : texte imprimé Auteurs : Xinya Zhang, Auteur ; Xia Wei, Auteur ; Wenxing Yang, Auteur ; Yongjin Li, Auteur ; Huanqin Chen, Auteur Année de publication : 2012 Article en page(s) : p. 765-774 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Acrylate de butyle
Analyse thermique
Caractérisation
Chromatographie sur gel
Copolymérisation
Latex
Méthacrylate de méthyle
Microscopie à force atomique
Microscopie électronique en transmission
Particules (matières)
Polyacrylates
Polymérisation en émulsion
Résonance magnétique nucléaireIndex. décimale : 667.9 Revêtements et enduits Résumé : In this study, gradient acrylate latex particles were synthesized by gradient copolymerization. n-Butyl acrylate (BA) and methyl methacrylate (MMA) were used as co-monomers, dodecyl diphenyl ether sodium disulfonate as the emulsifier, and potassium persulfate as the initiator. The technique involved the continuous addition of one monomer mixture into a stirred tank containing another monomer mixture. Their microstructure and properties were analyzed by nuclear magnetic resonance (NMR), gel permeation chromatography (GPC), differential scanning calorimetry (DSC), atomic force microscopy (AFM), transmission electron microscopy (TEM), and particle size analysis. 1H-NMR spectra show a gradual change in the chemical composition with the growth of molecular chains. The gradient polymer latexes, the instantaneous copolymer composition of which varies as the polymerization proceeds, are obtained. GPC analyses show that the aggregated molecular chain in the BA-centered latex particle experiences a process from dominant BA to MMA with changes in the monomer's feed composition during the polymerization. Particle size and TEM analyses show that the increasing latex size agrees well with the gradient growth pattern. DSC analyses show that the gradient latex particles exhibit only one broadened and ambiguous glass transition region. AFM analyses indicate no obvious microphase segregation occurs in the gradient latex particles, further verifying that the gradient microstructure is obtained. Note de contenu : - EXPERIMENTAL DETAILS : Materials - Gradient emulsion polymerization - Testing and characterization
- RESULTS AND DISCUSSION : H-NMR analyses - GPC analyses - Particle size analyses - DSC and MFT analyses - AFM analysesDOI : 10.1007/s11998-012-9422-x En ligne : https://link.springer.com/content/pdf/10.1007%2Fs11998-012-9422-x.pdf Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=16662
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PermalinkCharacterization of commercial polysorbates using different chromatographic techniques / S. Abrar in TENSIDE, SURFACTANTS, DETERGENTS, Vol. 46, N° 5/2009 (09-10/2009)
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