Assessment of the anti-hair loss potential of Camellia japonica fruit shell extract in vitro / Jiyoung You in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 2 (03-04/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 2 (03-04/2023) . - p. 155-165 Titre : Assessment of the anti-hair loss potential of Camellia japonica fruit shell extract in vitro Type de document : document électronique Auteurs : Jiyoung You, Auteur ; Jieun Woo, Auteur ; Kyung-Baeg Roh, Auteur ; Dehun Ryu, Auteur ; Youngsu Jang, Auteur ; Eunae Cho, Auteur ; Deokhoon Park, Auteur ; Eunsun Jung, Auteur Année de publication : 2023 Article en page(s) : p. 155-165 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Alopécie Camélia du Japon Camellia japonica, communément appelé camélia, est une espèce d'arbustes. C. japonica aurait été rapporté du Japon par les marchands portugais au XVIe siècle, puis à plusieurs reprises par les Britanniques en provenance de Chine, à la fin du XVIIIe siècle. Des tests in vitro laissent penser que Camellia japonica pourrait avoir des vertus protectrices de la peau (encore non comprise en 2019) contre la pollution de l'air. (Wikipedia) Cheveux -- Croissance Cheveux -- Soins et hygiène Cosmétiques Dihydrotestostérone Extraits de plantes:Extraits (pharmacie) Produits capillaires Stress oxydatif Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Hair loss is caused by various factors. Impacts of these factors are often overlapped and intensified. Currently, mitigation of hair loss is being studied by proliferating dermal papilla cells (DPCs) and inhibiting deleterious factors such as dihydrotestosterone (DHT) and oxidative stress on hair growth. Camellia japonica (C. japonica) fruit shell is a discarded part. Its biological activity remains to be elucidated. In this study, we investigated the capacity of C. japonica fruit shell extract (CJFSE) for hair loss mitigation. - Methods : MTT assay, spheroid culture and quantitative RT-PCR were performed to observe the proliferative effect of CJFSE on hair follicle dermal papilla cells (HFDPCs). Effects of CJFSE on DHT-induced hair loss were confirmed by Dkk-1 ELISA, β-galactosidase (β-gal) and 5α-reductase activity assay. In addition, effects of CJFSE on oxidative stress were confirmed through DPPH and ROS production assays. - Results : CJFSE increased the proliferation and spheroid size of HFDPCs. Expression levels of VEGF-A, Wnt-1, c-Myc and Cyclin D1 were upregulated by CJFSE. CJFSE also suppressed 5α-reductase activity and DHT-induced decrease in cell proliferation, Dkk-1 secretion and β-gal activity. Moreover, CJFSE showed DPPH scavenging activity and ameliorated hydrogen peroxide-induced ROS production and β-gal activity. Finally, gallic acid and protocatechuic acid were observed in CJFSE through HPLC analysis. - Conclusion : CJFSE has the potential to alleviate hair loss by promoting hair cell growth and suppressing effects of DHT and oxidative stress on hair Note de contenu : - MATERIALS AND METHODS : Cell culture - Preparation of Camellia japonica fruit shell extract - Cell viability assay - Cell proliferation assay - Spheroid culture of HFDPCs - Quantitative real-time PCR - Western blot analysis - DHT-induced senescence - 5α-reductase activity assay - Enzyme-linked immunosorbent assay (ELISA) - SA-β- gal staining - DPPH radical scavenging assay - H2O2-induced senescence - Analysis of ROS production - Phenolic compound analysis using HPLC - Statistical - RESULTS : CJFSE increases the proliferation of HFDPCs - CJFSE augments spheroid size - CJFSE induces the expression of VEGF and genes related to HFDPCs proliferation - CJFSE inhibits the effect of DHT on HFDPCs - CJFSE inhibits the effect of oxidative stress on HFDPCs - Gallic acid and protocatechuic acid are identified as phenolic compounds in CJFSE - Table 1 : Gene primers sequences for real-time PCR (5′ to 3′) DOI : https://doi.org/10.1111/ics.12827 En ligne : https://drive.google.com/file/d/1J3f1fwY-N2SQ3MiJgM5lDQqCExw-7ftd/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39166 [article]

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Melanogenesis-promoting effect of Cirsium japonicum flower extract in vitro and ex vivo / Minkyung Kim in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 43, N° 6 (12/2021)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 6 (12/2021) Titre : Melanogenesis-promoting effect of Cirsium japonicum flower extract in vitro and ex vivo Type de document : document électronique Auteurs : Minkyung Kim, Auteur ; Kyungeun Jeon, Auteur ; Seoungwoo Shin, Auteur ; Sohyun Yoon, Auteur ; Hayeon Kim, Auteur ; Hee Young Kang, Auteur ; Dehun Ryu, Auteur ; Deokhoon Park, Auteur ; Eunsun Jung, Auteur Année de publication : 2021 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Adénosine monophosphate cyclique L'adénosine monophosphate cyclique (ou AMP cyclique ou AMPc) est une molécule biologique qui agit souvent en tant qu'intermédiaire, dans l'action des hormones ou des neurotransmetteurs notamment. Elle fait partie des seconds messagers. Cosmétiques Extraits de plantes:Extraits (pharmacie) Follicule pileux Mélanogénèse Peau -- Soins et hygiène Peau reconstituée Pigmentation de la peau Pigmentation, Troubles de la Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : In this study, we examined the effect of C. japonicum flower extract (CFE) on melanogenesis and its mechanism in vitro and ex vivo. - Methods : The effect of CFE on melanogenesis was investigated with lightly (HEMn-LP) and moderately (HEMn-MP) pigmented normal human melanocytes, reconstituted three-dimensional skin (3D skin) model and ex vivo human hair follicles. The melanogenesis-inducing effect of CFE was evaluated using melanin content and intracellular tyrosinase activity assay. The amount and type of eumelanin and pheomelanin were analysed by using HPLC method. The mechanism involved in the effect of CFE on hyperpigmentation was explored by cyclic adenosine monophosphate (cAMP) immunoassay and western blot analysis for tyrosinase, microphthalmia-associated transcription factor (MITF) and phosphorylated CRE-binding protein (pCREB) expression. The degree of pigmentation in 3D skin and L-values were measured using a CR-300 chroma meter. The amount of dissolved melanin was measured using a spectrophotometer. The content of melanin in the hair follicles was evaluated by Fontana Masson staining. - Results : C. japonicum flower extract significantly increased the melanin content and cellular tyrosinase activity in both HEMn-LP and HEMn-MP cells. The markers of pheomelanin and eumelanin in HEMn-LP and HEMn-MP were also increased by CFE. We observed that CFE treatment on melanocytes increased intracellular cAMP with inducing pCREB and up-regulating the protein levels of TYR and MITF. Furthermore, CFE considerably increased the melanin content in a 3D skin model and ex vivo human hair follicles. - Conclusions : These results suggest that CFE exerts hyperpigmentation activity through cAMP signalling in human melanocytes that it can improve follicular depigmentation and vitiligo by stimulating the melanin synthesis. Note de contenu : - MATERIALS AND METHODS : Materials - Cell culture and viability assay - Melanin content and cell viability assay - Analysis of eumelanin and pheomelanin by HPLC - Cellular tyrosinase activity - Cyclic AMP immunoassay - Western blot analysis - The reconstituted three-dimensional human skin model - Ex vivo human hair follicles - Statistical analysis - RESULTS : Effect of CFE on the melanin content of lightly and moderately pigmented human melanocytes - Analysis on markers of pheomelanin and eumelanin - Effect of CFE on cellular tyrosinase activity - Effect of CFE on cAMP signalling pathway - Melanogenic effect of CFE on 3D skin model - Table 1 : TTCA and PTCA as specific markers in lightly and moderately pigmented melanocytes DOI : https://doi.org/10.1111/ics.12746 En ligne : https://drive.google.com/file/d/1ujuVw-VGc03qkR-B5zlcoeYHm0Vn4ygI/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37051 [article]

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