| Titre : |
Melanogenesis-promoting effect of Cirsium japonicum flower extract in vitro and ex vivo |
| Type de document : |
document électronique |
| Auteurs : |
Minkyung Kim, Auteur ; Kyungeun Jeon, Auteur ; Seoungwoo Shin, Auteur ; Sohyun Yoon, Auteur ; Hayeon Kim, Auteur ; Hee Young Kang, Auteur ; Dehun Ryu, Auteur ; Deokhoon Park, Auteur ; Eunsun Jung, Auteur |
| Année de publication : |
2021 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Adénosine monophosphate cyclique L'adénosine monophosphate cyclique (ou AMP cyclique ou AMPc) est une molécule biologique qui agit souvent en tant qu'intermédiaire, dans l'action des hormones ou des neurotransmetteurs notamment. Elle fait partie des seconds messagers.
Cosmétiques
Extraits de plantes:Extraits (pharmacie)
Follicule pileux
Mélanogénèse
Peau -- Soins et hygiène
Peau reconstituée
Pigmentation de la peau
Pigmentation, Troubles de la
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : In this study, we examined the effect of C. japonicum flower extract (CFE) on melanogenesis and its mechanism in vitro and ex vivo.
- Methods : The effect of CFE on melanogenesis was investigated with lightly (HEMn-LP) and moderately (HEMn-MP) pigmented normal human melanocytes, reconstituted three-dimensional skin (3D skin) model and ex vivo human hair follicles. The melanogenesis-inducing effect of CFE was evaluated using melanin content and intracellular tyrosinase activity assay. The amount and type of eumelanin and pheomelanin were analysed by using HPLC method. The mechanism involved in the effect of CFE on hyperpigmentation was explored by cyclic adenosine monophosphate (cAMP) immunoassay and western blot analysis for tyrosinase, microphthalmia-associated transcription factor (MITF) and phosphorylated CRE-binding protein (pCREB) expression. The degree of pigmentation in 3D skin and L-values were measured using a CR-300 chroma meter. The amount of dissolved melanin was measured using a spectrophotometer. The content of melanin in the hair follicles was evaluated by Fontana Masson staining.
- Results : C. japonicum flower extract significantly increased the melanin content and cellular tyrosinase activity in both HEMn-LP and HEMn-MP cells. The markers of pheomelanin and eumelanin in HEMn-LP and HEMn-MP were also increased by CFE. We observed that CFE treatment on melanocytes increased intracellular cAMP with inducing pCREB and up-regulating the protein levels of TYR and MITF. Furthermore, CFE considerably increased the melanin content in a 3D skin model and ex vivo human hair follicles.
- Conclusions : These results suggest that CFE exerts hyperpigmentation activity through cAMP signalling in human melanocytes that it can improve follicular depigmentation and vitiligo by stimulating the melanin synthesis. |
| Note de contenu : |
- MATERIALS AND METHODS : Materials - Cell culture and viability assay - Melanin content and cell viability assay - Analysis of eumelanin and pheomelanin by HPLC - Cellular tyrosinase activity - Cyclic AMP immunoassay - Western blot analysis - The reconstituted three-dimensional human skin model - Ex vivo human hair follicles - Statistical analysis
- RESULTS : Effect of CFE on the melanin content of lightly and moderately pigmented human melanocytes - Analysis on markers of pheomelanin and eumelanin - Effect of CFE on cellular tyrosinase activity - Effect of CFE on cAMP signalling pathway - Melanogenic effect of CFE on 3D skin model
- Table 1 : TTCA and PTCA as specific markers in lightly and moderately pigmented melanocytes |
| DOI : |
https://doi.org/10.1111/ics.12746 |
| En ligne : |
https://drive.google.com/file/d/1ujuVw-VGc03qkR-B5zlcoeYHm0Vn4ygI/view?usp=shari [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37051 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 6 (12/2021)
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