Reproducing the scalp microbiota community : co-colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta / Marisa Meloni in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 43, N° 2 (04/2021)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 2 (04/2021) . - p. 235-245 Titre : Reproducing the scalp microbiota community : co-colonization of a 3D reconstructed human epidermis with C. acnes and M. restricta Type de document : document électronique Auteurs : Marisa Meloni, Auteur ; Silvia Balzaretti, Auteur ; Nathalie Collard, Auteur ; Stéphane Desaint, Auteur ; Céline Laperdrix, Auteur Année de publication : 2021 Article en page(s) : p. 235-245 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cuir chevelu Dermatologie Justification de l'allégation Microbiologie Microbiome cutané Pellicules (dermatologie) Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : A 3D reconstructed human epidermis (RHE) model colonized with specific microbial strains was developed to model the complex interactions between strains of the human scalp hair. - Methods : Reconstructed human epidermis was colonized with Cutibacterium acnes and Malassezia restricta for 72 h. The epidermal model was characterized in terms of morphology, using immune-labelling targeting biomarkers for barrier structure, proliferation, differentiation and anti-microbial defence. The barrier function was assessed by transepithelial electrical eesistance (TEER) measurements. In order to study the microorganisms on the epidermal model, viable counts and phenotype ultrastructure analysis were performed by scanning electron microscopy (SEM). - Results : The RHE colonized with C. acnes did not lead to severe modifications of the physiological barrier integrity and viability, though it shows aggregates. M. restricta formed large aggregates by a close interaction with the RHE, thus causing both a strong decrease in barrier function and structure degradation and an increased human beta defensin 2 (HBD2) expression. The co-colonized model resulted in barrier depletion, but the overall damage was less severe, respecting the single colonization with M. restricta. The developed ‘scalp model’ allowed to identify morphological modifications leading to uncontrolled epidermal renewal. - Conclusion : This study shows a pre-clinical model that recapitulates the interactions that can occur between site-specific microbial strains and keratinocytes in dandruff condition. The model can be applied to assess ingredients and products’ mechanism of action. Note de contenu : - MATERIALS AND METHODS : Test system - C. acnes and M. restricta inoculum - RHE colonization - Bacteria and yeast viable counting - Transepithelial electrical resistance (TEER) measurements - Histo-morphological analysis: haematoxylin and eosin (H&E) staining - Immunofluorescence staining - Scanning electron microscopy (SEM) - RESULTS : Skin integrity : TEER measurements - Barrier structure and function: morphology by H&E staining - Epidermal differentiation : KRT 10 and KRT 14 immunofluorescence - Epidermal barrier and Stratum Corneum proteins expression : involucrin and loricrin immunofluorescence - Innate and anti-microbial response : beta defensin and elafin immunofluorescence - RHE colonization - Table 1 : Transepithelial electrical resistance (TEER) measurements expressed in Ohm*cm2 at basal time (t = 0 h) and after 72 h incubation. Duplicate biological samples - Table 2 : Viable count on the apical compartment after 72 h colonization with C. acnes, M. restricta and co-colonization DOI : https://doi.org/10.1111/ics.12688 En ligne : https://drive.google.com/file/d/1rtvUS5BBX75BnZj43mJbvG0fpW3eusVP/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35875 [article]

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