Quantitative ion determination in eccrine sweat gland cells correlates to sweat reduction of antiperspirant actives / J. Welzel in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 43, N° 2 (04/2021)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 2 (04/2021) . - p. 181-190 Titre : Quantitative ion determination in eccrine sweat gland cells correlates to sweat reduction of antiperspirant actives Type de document : document électronique Auteurs : J. Welzel, Auteur ; S. Grüdl, Auteur ; T. Welss, Auteur ; M. Claas, Auteur ; A. Sättler, Auteur ; T. Förster, Auteur ; B. Banowski, Auteur Année de publication : 2021 Article en page(s) : p. 181-190 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antiperspirants Biomolécules actives Cellules -- Cultures et milieux de culture Cosmétiques Formulation (Génie chimique) Ions Peau -- Soins et hygiène Statistique Transpiration Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Axillary wetness represents an unwanted effect of the physiologically vital sweating mechanism, especially when it becomes excessive. Cosmetic products reducing sweat secretion rely on aluminium salts as the active ingredient acting by physically blocking the sweat gland. Driven by the interest to better understand the sweat mechanism and to develop alternative technologies against excessive sweating a search for an effective testing approach started as up to now, cost- and time-consuming in vivo studies represent the standard procedure for testing and identifying these alternatives. Material and methods : The herein described in vitro test system is based on the measurement of intracellular changes of the ion equilibrium in cultured eccrine sweat gland cells. Subsequently, in vivo studies on the back of volunteers were conducted to verify the sweat-reducing effect of in vitro newly discovered substance. - Results : In this study, we describe an effective cell-based in vitro method as a potent tool for a more targeted screening of alternatives to aluminium salts. Testing the commonly used aluminium chlorohydrate as one example of an aluminium-based active in this screening procedure, we discovered a distinct influence on the ion equilibrium: Intracellular levels of sodium ions were decreased while those of chloride increased. Screening of various substances revealed a polyethyleneimine, adjusted to pH 3.5 with hydrochloric acid, to evoke the same alterations in the ion equilibrium as aluminium chlorohydrate. Subsequent in vivo studies showed its substantial antiperspirant action and confirmed the high efficiency of the polyethyleneimine solution in vivo. Further, specific investigations connecting the chloride content of the tested substances with the resulting sweat reduction pointed towards a substantial impact of the chloride ions on sweating. - Conclusion : The newly described in vitro cell-based screening method represents an effective means for identifying new antiperspirant actives and suggests an additional biological mechanism of action of sweat-reducing ingredients which is directed towards unbalancing of the ion equilibrium inside eccrine sweat gland cells. Note de contenu : - MATERIALS AND METHODS : Ethics statement - Cell culture - Determination of cell viability - Ion flux assays with fluorescence dyes - Statistical analysis - RESULTS : In vitro ion profile of the benchmark ACH - Concentration-dependent effect of the benchmark ACH - In vitro data reveal potent candidate - pH-dependent effect of PEI-HCl - In vivo sweat reduction efficacy of PEI-HCl - Chloride equivalent of ACH and PEI-HCl and its correlation to sweat reduction DOI : https://doi.org/10.1111/ics.12679 En ligne : https://drive.google.com/file/d/1Xj2duKMdT1kPtoQtaI4_lyiq19LF5k3J/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35761 [article]

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