| Titre : |
Topically applied, fatty acid-containing formulations provide superior barrier benefits in an ex vivo tape-stripped skin model |
| Type de document : |
texte imprimé |
| Auteurs : |
John Nip, Auteur ; Hilal Ilarslan, Auteur ; Ana Villa, Auteur ; Dawn Mihalov, Auteur ; Manoj Misra, Auteur ; Samantha D. Samaras, Auteur ; Lin Feng, Auteur ; Stella Arcella, Auteur ; John Bajor, Auteur ; Andrew E. Mayes, Auteur |
| Année de publication : |
2024 |
| Article en page(s) : |
p. 506-515 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Acides gras
Barrière cutanée
Couche cornée
Dermo-cosmétologie
Dermo-pharmacie
Etude ex vivo
Formulation (Génie chimique)
Lamelles lipidiques
Lipides
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- OBJECTIVE : Ex vivo skin has been used to study various skin conditions from atopic dermatitis to burn injury. The aim of this research is to identify a more effective barrier improvement strategy and to evaluate topical formulations in replenishing the skin. The skin can create new longer chain fatty acids and ceramides (CERs) from topically applied skin natural fatty acid to help renew the skin's barrier.
- METHODS : An ex vivo skin model damaged by sequential tape stripping of the stratum corneum (SC) was used to investigate the repair of the SC. Confocal laser scanning microscopy was used to assess the SC layers recovered. Ultrastructural analysis was performed using transmission electron microscopy to visualize the lamellar bodies and intercellular lipid lamellae.
- RESULTS : The data in this study provide the first direct ex vivo evidence comparing different marketed formulations containing three CERs with those containing fatty acids. Free fatty acid (FFA)-containing formulations, but not CER-containing formulations, directly applied to the damaged skin, showed an increased number of repaired SC layers and this was reflected at the ultrastructural level by an increased intercellular lipid lamellae length and an increased number of lamellar bodies.
- CONCLUSION : These findings demonstrate that FFA-containing formulations can repair damaged ex vivo skin and point to a repair mechanism in which topically applied palmitic and stearic acids, (which boost lipid levels and elongation) can increase the production and transport of lipids into a repaired SC and thus rebuild an effective skin barrier. |
| Note de contenu : |
- MATERIALS AND METHODS : Formulations - Ex vivo skin, tape-stripping and treatment - Biopsies of treated areas - Biopsy collection and processing for histology and cornified layer analysis - H&E staining of sections - Immunohistochemistry for Ki67 - Imaging and quantitation of epidermal area and Ki67 staining - Corneocyte layer quantification - Sample preparation for transmission electron microscopy (TEM) - Quantitative analysis of lamellar bodies (LB) and intercellular lipid lamellae (ICLL) - Statistical analyses
- RESULTS : Ex vivo skin response to tape stripping - Removal of SC and its recovery in ex vivo skin - Topical free fatty acid-containing formulations (FFA) increased SC layer recovery - Topical free fatty acid-containing formulations (FFA) increased intercellular lipid lamella (ICLL) formation and length - Topical FFA treatment stimulates lamellar body production and secretion |
| DOI : |
https://doi.org/10.1111/ics.12961 |
| En ligne : |
https://drive.google.com/file/d/1dBCIelfqOUS_L1KmdXWBuOYVPw4fintT/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=41350 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 46, N° 4 (08/2024) . - p. 506-515
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Topically applied, fatty acid-containing formulations provide superior barrier benefits in an ex vivo tape-stripped skin model in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 46, N° 4 (08/2024)
