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Auteur Cristina Sánchez-Porro
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Faculty of Pharmacy - University of Sevilla - Spain
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Characterization of moderately halophilic bacteria from salt-pack-cured hides / Pinar Caglayan in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 99, N° 5 (09-10/2015)
[article]
Titre : Characterization of moderately halophilic bacteria from salt-pack-cured hides Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Cristina Sánchez-Porro, Auteur ; Antonio Ventosa, Auteur ; Meral Birbir, Auteur Année de publication : 2015 Article en page(s) : p. 250-254 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bactéries -- Identification
Bactéries -- Inactivation
Caractérisation
Cuirs et peaux -- Conservation
Peaux brutes -- SalageIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : In the present study, all hide sample contained moderately halophilic bacteria and 13 different species which belong to the genera of Salimicrobium, Halomonas, Alkalibacillus, Marinococcus, Oceanobacillus, Thalassobacillus, Chromohalobacter were isolated and identified with phenotypic analyses and molecular methods. It was found that moderately halophilic isolates perform biochemical processes to breakdown macromolecules such as protein, DNA, fats which are responsible for hides deterioration. Therefore, we suggest steps should be taken to examine and inactivate moderately halophilic bacteria in preservation salt to obtain high quality leather. Note de contenu : - Sample collection and growth conditions
- Determination of total number of moderately halophilic bacteria
- Biochemical tests of the isolates
- Amplification and sequencinf of 16S rRNA genes
- Nucleotides accession numberEn ligne : https://drive.google.com/file/d/1Y8FLn69ezJ_y6EhLoR8iaySh7HFtQ9M7/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24656
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 99, N° 5 (09-10/2015) . - p. 250-254[article]Réservation
Réserver ce documentInvestigation of moderately halophilic bacteria causing deterioration of the salted sheep and goat skins and their extermination via electric current applications / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIII, N° 2 (02/2018)
[article]
Titre : Investigation of moderately halophilic bacteria causing deterioration of the salted sheep and goat skins and their extermination via electric current applications Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Cristina Sánchez-Porro ; Antonio Ventosa ; Yasar Birbir Année de publication : 2018 Article en page(s) : p. 41-52 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries
Courants alternatifs
Courants électriques
Cuirs et peaux -- Analyse
Cuirs et peaux -- Conservation
Cuirs et peaux de chèvres
Cuirs et peaux de moutonsIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Determination of moderately halophilic bacterial counts on the salted skins and examination of utilization of amino acid and carbon sources by skin isolates offer important information about biodegradation of salted skins. Hence, total counts of moderately halophilic bacteria, total counts of proteolytic and lipolytic moderately halophilic bacteria found on the salted sheep and goat skins belonging to different countries were examined in this study. One hundred-thirty seven moderately halophilic bacterial species closely related to species of genera Alkalibacillus, Bacillus, Chromohalobacter, Gracilibacillus, Halomonas, Idiomarina, Marinococcus, Oceanobacillus, Planococcus, Salimicrobium, Salinicoccus, Staphylococcus and Salinivibrio were used to detect utilization of different amino acids and carbon sources which are related to biodegradation of skins. The values of pH, moisture contents, ash contents and salt saturations of these skins were also investigated to understand the correlation between these parameters and moderately halophilic bacterial activities. All salted skin samples contained moderately halophilic bacteria, proteolytic and lipolytic moderately halophilic bacteria in high numbers. Each of 137 test isolates, obtained from the salted sheep and goat skins, used different amino acids found in the skin structure. While 100% of both sheep and goat skin isolates utilized L-arginine, 86%, 66%, 85%, 64% and 66% of the isolates respectively utilized L-glycine, L-alanine, L-tyrosine, L-proline and L-hydroxyproline amino acids. Ninety-three percent of the isolates used different carbon sources such as dulcitol (13%), D-sorbitol (29%), L-rhamnose (19%), ribose (52%), salicin (40%), myo-inositol (27%), xylitol (21%), benzoate (18%), propionate (34%), D-melezitose (31%), butanol (20%), propanol (23%), methanol (23%), formate (23%) and tartrate (12%). While pH values, moisture contents, ash contents and salt saturations of the sheep skins ranged from 6.53 to 8.01, 32 to 68%, 12 to 30% and 58 to 100%, respectively, the pH values, moisture contents, ash contents and salt saturations of the goat skins were between 6.65-8.06, 34-70%, 11-32% and 64-100%, respectively. The values of all skin samples were found to be suitable for the growth and catabolic activities of these bacteria. To prevent the growth and activities of moderately halophilic isolates causing skin deterioration, the annihilation effects of 0.5, 1.0, 1.5 and 2.0 A direct and alternating electric current treatments on the mixed culture of moderately halophilic isolates (Chromohalobacter israelensis, Chromohalobacter canadensis, Halomonas halodenitrificans, Staphylococcus nepalensis and Halomonas halmophila) were also separately investigated in the present study. Both 0.5, 1.0, 1.5, 2.0 A direct and alternating electric current applications used in this study completely annihilated the mixed culture of moderately halophilic spoilage microorganisms. As a conclusion, we suggest using lowlevel direct or alternating electric current treatment to eradicate harmful moderately halophilic bacteria in salt which will be used in brine curing of hides and skins in the leather industry. Note de contenu : - EXPERIMENTAL : Skin Samples - Determination of total of moderately halophilic bacteria on the skin samples - Determination of total counts of proteolytic and lipolytic moderately halophilic bacteria on the skins - Utilization of different amino acids and different carbon sources - Determination of pH values of the salted skins - Determination of moisture content, ash content and salt saturation of the salted skins - Application of alternating and direct electric currents on the mixed culture
- RESULTS AND DISCUSSION : Moderately halophilic bacteria, proteolytic and lipolytic bacteria on salted sheep and goat skin samples - Determination of pH value, moisture content, ash content and salt saturation of the salted skins - Utilization of different amino acids and different carbon sources - Application of alternating and direct electric currents on the mixed culture of moderately halophilic spoilage bacteriaEn ligne : https://drive.google.com/file/d/13FYlhwuWlVz9ABkOdZFqz5LbN0xD_nh_/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=29950
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIII, N° 2 (02/2018) . - p. 41-52[article]Réservation
Réserver ce documentMolecular identification of moderately halophilic bacteria and extremely halophilic archaea isolated from salted sheep skins containing red and yellow discolorations / C. Akpolat in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CX, N° 7 (07/2015)
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Titre : Molecular identification of moderately halophilic bacteria and extremely halophilic archaea isolated from salted sheep skins containing red and yellow discolorations Type de document : texte imprimé Auteurs : C. Akpolat, Auteur ; Antonio Ventosa, Auteur ; Meral Birbir, Auteur ; Cristina Sánchez-Porro, Auteur ; Pinar Caglayan, Auteur Année de publication : 2015 Article en page(s) : p. 211-220 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification
Cuirs et peaux -- Analyse
Cuirs et peaux de moutons
Décoloration
Peaux brutes -- Détérioration
Peaux brutes -- SalageIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Salted hides or skins containing red and yellow discolorations have been examined for many years, but much less information is available about the isolation and molecular techniques for identifying moderately halophilic bacteria and extremely halophilic archaea on the salted sheep skins exhibiting these blotches. The deteriorated salted sheep skins were collected from a warehouse in Spain. Moderately halophilic bacteria and extremely halophilic archaea were isolated from these samples and molecular identification of these microorganisms were performed using 16S rRNA gene sequence analysis. Total cell counts of moderately halophilic bacteria and extremely halophilic archaea were found as 105-108 CFU/g and 105-107 CFU/g, respectively. According to comparative partial 16S rRNA gene sequence analysis, Alkalibacillus halophilus, Pseudomonas halophila, Acinetobacter johnsonii, Alkalibacillus salilacus, Salimicrobium salexigens, Marinococcus luteus and Staphylococcus equorum subsp. equorum belonging to moderately halophilic bacteria; and Halorubrum tebenquichense, Halorubrum saccharovorum, Halococcus dombrowskii, Halococcus qingdaonensis, Natrinema pellirubrum, Halococcus morrhuae, Halorubrum kocurii, Halorubrum terrestre, Halorubrum lipolyticum, Halostagnicola larsenii, Haloterrigena saccharevitans and Natrinema versiforme belonging to extremely halophilic archaea were isolated from these sheep skins. Alkalibacillus halophilus belonging to moderately halophilic bacteria and Halorubrum tebenquichense belonging to extremely halophilic archaea were found as the most common species on the skins. Among the moderately halophilic bacterial isolates, Acinetobacter johnsonii showed lipolytic activities. Among the extremely halophilic archaeal isolates, Halococcus dombrowskii, Halococcus morrhuae, Natrinema pellirubrum, Halorubrum lipolyticum showed proteolytic activity and Halococcus dombrowskii, Halorubrum lipolyticum, Haloterrigena saccharevitans, Natrinema versiforme showed lipolytic activity. Hair slip, red and yellow discolorations, slimy layers and bad odor were detected on the skin samples examined. This study confirmed that salted sheep skins were contaminated by preservation salt containing different proteolytic or lipolytic species of mostly extremely halophilic archaea. Therefore, antimicrobial applications during brine curing of skins should be applied to overcome halophilic microbial damage on the salted skins. Note de contenu : - Collection of salted skins
- Determination of pH values of the salted skins
- Determination of moisture content of the salted skins
- Determination of total bacterial population of moderately halohilic bacteria and extremely halophilic archaea on the skin samples
- Morphological, cultural and physiological characteristics of the isolates
- Determination of protease activity
- Determination of lipase production
- Phylogenetic analysis
- Nucleotide accessionEn ligne : https://drive.google.com/file/d/1_NYcWNCVWPoaRDNsUI_pJ6gxrv5e2xHc/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=24374
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CX, N° 7 (07/2015) . - p. 211-220[article]Réservation
Réserver ce documentScreening of industrially important enzymes produced by moderately halophilic bacteria isolated from salted sheep skins of diverse origin / Pinar Caglayan in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXII, N° 6 (06/2017)
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Titre : Screening of industrially important enzymes produced by moderately halophilic bacteria isolated from salted sheep skins of diverse origin Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Cristina Sánchez-Porro, Auteur ; Antonio Ventosa, Auteur Année de publication : 2017 Article en page(s) : p. 207-216 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Bactéries -- Identification
Cuirs et peaux de moutons
Enzymes microbiennesIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Moderately halophilic bacteria have received attention in several industries due to their industrial enzymes which are stable at high temperatures, various salt concentrations and different pH values. Therefore, this study was conducted to isolate and identify moderately halophilic bacteria found on salted sheep skin samples and to detect the isolates producing industrially important enzymes. These skin samples were from Australia, Bulgaria, Dubai, Greece, Israel, Kuwait, South Africa, Turkey and U.S.A. Phenotypic characteristics and comparative partial 16S rRNA gene sequence analysis were used to characterize these microorganisms. According to the test results, 77 isolates representing 13 genera and 29 species were identified. These moderately halophilic bacteria, which were able to mostly grow in the media containing 3-15% salts and in some cases, up to 20-25% salts, were closely related to species of genera Staphylococcus, Salimicrobium, Bacillus, Salinicoccus, Planococcus, Alkalibacillus, Gracilibacillus, Oceanobacillus, Marinococcus, Halomonas, Salinivibrio, Chromohalobacter, and Idiomarina. A fairly high percentage of the isolates (79%) produced a great variety of industrially important enzymes. Protease, lipase, ?-galactosidase, amylase, caseinase, DNase, urease, cellulase, and lecithinase enzymes were produced respectively by 46, 33, 30, 20, 18, 13, 9, 9, and 8 isolates. None of the isolates produced pullulanase, xylanase and phospholipase enzymes. Combined enzymatic activities have been detected among the isolates. While 12% and 27% of isolates produced six and four different enzymes, respectively, 1% of isolates produced three different enzymes. Furthermore, 39% of the isolates produced one and two enzymes. These enzymes were produced by isolates belonging to all genera detected in this study except genus Alkalibacillus. The findings of this study demonstrated that moderately halophilic bacteria on the skins produced industrially important enzymes which may have potential applications in different industries. Note de contenu : - Salted sheep skin samples
- Isolation of moderately halophilic bacteria
- Amplification and sequencing of 165 rRNA genes of the isolates
- Morphological, cultural and physiological characteristics of the isolates
- Determination of enzymatic activities of the isolatesEn ligne : https://drive.google.com/file/d/1OONpPvQ2DeG_y2jzFrMDY7mjX878p-zJ/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=28691
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXII, N° 6 (06/2017) . - p. 207-216[article]Réservation
Réserver ce documentThe effects of alternating and direct electric currents on moderately halohilic bacteria in leather industry / Pinar Caglayan in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 100, N° 6 (11-12/2016)
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Titre : The effects of alternating and direct electric currents on moderately halohilic bacteria in leather industry Type de document : texte imprimé Auteurs : Pinar Caglayan, Auteur ; Meral Birbir, Auteur ; Ayse Ogan, Auteur ; Antonio Ventosa, Auteur ; Cristina Sánchez-Porro, Auteur ; Yasar Birbir, Auteur Année de publication : 2016 Article en page(s) : p. 307-313 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Antimicrobiens
Bactéries -- Inactivation
Courants électriques
Cuirs et peaux -- Conservation
Cuirs et peaux de moutonsIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Proteolytic and lipolytic moderately halophilic bacteria, originate from salt, may be found on salted sheep skins. Microbial spoilage of sheepskin usually result a from the failure to inactivate proteolytic and Iipolytic micro-organisms during skin preservation. To solve this problem, an effective inactivation method to be used in the leather industry was examined in this study. Protease and lipase activities of moderately halophilic Staphylococcus saprophyticus subsp. saprophyticus (TR5), Bacilius pumilus (DB5), Bacillus licheniformis (DBA2), Gracilibacillus dipsosauri (EK2) and Idiomarina loihiensis (GA3), isolated from salted sheepskins, were examined : Protease activity of each test isolate was determined by bath gelatin agar medium and casein digestion assay at 280nm. Lipolytic activity of each test isolate was determined by bath Tween 80 agar medium and measuring hydrolysis of p-nitrophenylbutyrate to p-nitrophenol at 405nm. Antibiotic sensitivities of test isolates to novobiocin, penicillin G, tetracycline, streptomycin, erythromycin, tobramycin, kanamycin and gentamicin were tested using disc diffusion susceptibility method. These isolates were found to be proteolytic, lipolytic and resistant to aforementioned antibiotics. inactivation effects of direct electric currents and alternating electric currents (0.5A, 1.0A, 1: 5A and 2.0A) on the mixed culture of these isolates were separately examined in liquid medium containing 25% NaCl. The mixed culture was killed in 1 minute by 0.5A direct current and 1.5A affernating current treatments. However, 0.5A alternating current and 1.0A alternating current treatments eliminated these micro-organisms respectively in 15 minutes and 10 minutes. In conclusion, electric current applications hold great potentiel for the eradication of proteolytic and lipolytic, antibiotic-resistant, moderately halophilic bacteria in leather industry. Note de contenu : - EXPERIMENTAL PROCEDURES : Moderately halophilic bacterial isolates - Proteolytic and lipolytic activities of moderately halophilic bacteria - Antimicrobial susceptibility test - The electrochemical treatment system
- RESULTS AND DISCUSSION : Antibiotic resistanceEn ligne : https://drive.google.com/file/d/1694CJoD9Uwle2gArKMHAUoJyAkxmVqSI/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27666
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 100, N° 6 (11-12/2016) . - p. 307-313[article]Réservation
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