Bright fluorescent dsDNA probes : novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine-quinolinium chromophore / Aleksey Vasilev in COLORATION TECHNOLOGY, Vol. 131, N° 2 (04/2015)  

[article]  inCOLORATION TECHNOLOGY > Vol. 131, N° 2 (04/2015) . - p. 94-103 Titre : Bright fluorescent dsDNA probes : novel polycationic asymmetric monomethine cyanine dyes based on thiazolopyridine-quinolinium chromophore Type de document : texte imprimé Auteurs : Aleksey Vasilev, Auteur ; Nedyalko Lesev, Auteur ; Sonya Dimitrova, Auteur ; Marina Nedelcheva-Veleva, Auteur ; Stoyno Stoynov, Auteur ; Silvia Angelova, Auteur Année de publication : 2015 Article en page(s) : p. 94-103 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : ADN Analyse spectrale Colorants Colorants -- Synthèse Diagnostic biologique Electrophorèse Fluorescence Index. décimale : 667.3 Teinture et impression des tissus Résumé : Seven tri- and tetracationic monomeric and homodimeric monomethine cyanine dyes based on thiazolo[4,5-b]pyridinium and quinolinium end groups were synthesised and characterised. The dyes were tested as fluorescent DNA intercalating probes to apply in DNA gel electrophoresis. The DNA samples stained with all dyes from the series demonstrated bright fluorescent signals. DNA fragments were successfully visualised under orange and green filters as well as under standard UV transillumination. Two of the studied dyes revealed higher sensitivity to DNA when compared with the commercial dimeric cyanine dye TOTO-1. Their sensitivity reached that of the commercial dimeric cyanine dye YOYO-1, but the emission was shifted to longer wavelengths. These qualities make the dyes suitable to apply in a wide range of medical and scientific analytical methods. Note de contenu : - EXPERIMENTAL : Materials - Equipment - Synthesis of dye 3 - Synthesis of tri- and tetracationic dyes 5a to 5g - Preparation of dyes and DNA solutions - Staining after electrophoresis - Staining prior to electrophoresis - RESULTS AND DISCUSSION : Synthesis and structural characterisation of dyes 5a to 5g - Spectroscopic characteristics of the dyes and computational study of the chromophore before binding to dsDNA - Spectroscopic characteristics after binding to dsDNA - Staining after electrophoresis completion - Staining DNA samples prior to electrophoresis - Retardation of the mobility of stained DNA fragments DOI : 10.1111/cote.12133 En ligne : https://onlinelibrary.wiley.com/doi/epdf/10.1111/cote.12133 Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=23656 [article]

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