Titre : |
The measurement of the binding of tannin subunits to soluble collagen by continuous-flow dynamic dialysis |
Type de document : |
texte imprimé |
Auteurs : |
Neil A. Sparrow, Auteur ; Alan E. Russell, Auteur ; Leslie Glasser, Auteur |
Année de publication : |
1982 |
Article en page(s) : |
p. 97-106 |
Note générale : |
Bibliogr. |
Langues : |
Anglais (eng) |
Catégories : |
Analyse mathématique Collagène Dialyse dynamique à flux continu Dynamique moléculaire Ligands Polyphénols Protéines Tanins végétaux
|
Index. décimale : |
675.2 Préparation du cuir naturel. Tannage |
Résumé : |
Studies of the binding of low molecular mass analogues of polyphenolic vegetable tannins to soluble hide collagen have prompted the development of a novel method for investigating protein—ligand interactions. This method, referred to as continuous-flow dynamic dialysis (CFDD), differs from conventional equilibrium dialysis procedures for measurement of protein—ligand binding in that the ligand concentration in the diffusate is monitored automatically at successive closely spaced time intervals, while being continuously eluted from a dialysis cell. Details of the method and its validation using a bovine serum albumin—phenol red binding system are reported. The method has been applied to investigate the binding to collagen of various phenolic tannin subunits which can be considered as monomeric precursor analogues of the polymeric vegetable tannins. Binding of these ligands to collagen is shown to be characterized by high capacity, low affinity binding in which ligand uptake increases Iinearly with concentration. No indication of site saturation was observed over the experimentally accessible ligand concentration range. |
Note de contenu : |
- EXPERIMENTAL : Continuous-flow dynamic dialysis (CFDD)
- MATERIALS : Phanol red-BSA interaction - Collagen-plant phenol interactions
- MATHEMATICAL ANALYSIS
- RESULTS : Phenol red-BSA binding
- Figure : Structures of the monomeric tannin precursor molecules
- Table 1 : Experimental data for the dialysis of monomeric tannin precursors at 15°C
- Table 2 : Binding parameters for the phenol red-BSA interaction at 25°C
- Table 3 : Permeation constants for plant phenolic ligands
- Table 4 : Coefficients for the linear equation fitted to binding isotherm data |
En ligne : |
https://drive.google.com/file/d/1HA8Jw8XcJU6RDJPHj8_qt2bmglbP80sU/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34214 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 66 (Année 1982) . - p. 97-106