Titre : |
Biocatalytic coatings - Multi-layer, permeable, reactive latex coatings containing living bacteria : Applications for biosensors, bio-electronic devices and biocatalysis |
Type de document : |
texte imprimé |
Auteurs : |
Praful Sanjay Dahatonde, Auteur |
Année de publication : |
2014 |
Article en page(s) : |
p. 63-65 |
Note générale : |
Bibliogr. |
Langues : |
Anglais (eng) |
Catégories : |
Bactéries Biocapteurs Biocatalyse Latex Perméabilité Revêtements multicouches
|
Index. décimale : |
667.9 Revêtements et enduits |
Résumé : |
Biocatalytic coatings are thin (<100µm), nano porous, coatings of partially coalesced polymer particles surrounding living bacteria. Coatings are formulated so that they can be dried and frozen without loss of microbial activity. Whole-cell biosensors, oxidations and reductions have been demonstrated in coatings consisting of a 30µm to 50µm thick bacteria plus latex layer covered by a porous sealant coating. Formulation and drying methods have been developed to generate permeability without killing the cells. A film formation toxicity assay is used to optimize dying and reactivity. Permeability is monitores by diffusion measurements and cryo-SEM. Bacteria such as E.coli are viable and capable of gene expression and coating, drying, storage and rehydration. Spatial gene expression is monitored using gene fusions and LSCM. Coatings have been formulated for hyperthermopiles, facultative anaerobes and aerobic bacteria. A high effectiveness factor coating for the oxidation of D-sorbitol L-sorbose by latex-embedded Gluconobacter is being studied for developing coating methods for strict aerobes. A diffusion reaction model is proposed to engineer highly reactive multi-layer bacterial coatings for a variety of applications. |
Note de contenu : |
- Why develop coatings of non-growing micro organisms preserved at ambient temperature?
- Development of robust porous materials containing living micro organisms |
En ligne : |
https://drive.google.com/file/d/1c9rt4oJvVTKok3bNPfQrlJTfpCZ8BriF/view?usp=drive [...] |
Format de la ressource électronique : |
Pdf |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=22116 |
in PAINTINDIA > Vol. LXIV, N° 7 (07/2014) . - p. 63-65