[article]
Titre : |
Protection of human epidermal cells from UV-induced DNA damage by acetyl hexapeptide-1, a biomimetic peptide analog of α-melanocyte-stimulating hormoneα |
Type de document : |
texte imprimé |
Auteurs : |
Estelle Loing, Auteur ; Thierry Suère, Auteur |
Année de publication : |
2012 |
Article en page(s) : |
p. 89-95 |
Note générale : |
Bibliogr. |
Langues : |
Anglais (eng) |
Catégories : |
ADN -- Réparation Hexapeptides Protection cutanée
|
Index. décimale : |
668.5 Parfums et cosmétiques |
Résumé : |
Cutaneous pigmentation by melamins represents the main pathway to protect skin cells against the carcinogenic and ageing effects resulting from UV exposure. α-melanocyte-stimulating hormone (α-MSH) induces epidermal pigmentation upon binding to the melanocortin-1 receptor, which in turn activates the signal transduction pathway taht leads to the induction of tyrosinase and other key players for melanin synthesis. In addition, it has been proven that α-MSH directly reduces UV-induced DNA damage and enhances DNA repair in epidermal melanocytes and keratinocytes by modulating the function of DNA repair molecules.
Therefore, there has been increasing interest from skincare companies in developing α-MSH analogs that induce sunless tanning, with a particular emphasis on small peptide analogues for their ability to activate the melanocortin-1 receptor, reduce UV-induced DNA damage and easili penetrate across the stratum corneum after topical application. In this study we demonstrate that acetyl hexapeptide-1, a biomimetic peptide analogue of α-MSH, reduces the genotoxic adverse effects of both UVA and UVB in epidermal melanocytes, and that it protects human epidermal keratinocytes from DNA oxidative damages induced by UVA. Furthemore, using an ex vivo skin model, we show that acetyl hexapeptide-1 significantly reduces the appearance of cyclobutane pyrimidine dimers after UV challenge and that it blocks UV-induced caspase-3 activation and apoptosis. |
Note de contenu : |
- EXPERIMENTAL : Cell and tissue culture - Comet assay - Detection of 8-oxo-2'-deoxyguanosine (8-oxo-dG) - Immunohistochemical detection of cyclobutane pyrimidine dimers and caspase-3 - Image quantification by picture analysis - Evaluation of the anti-erythemal properties of acetyl hexapeptide-1 in human volunteers
- RESULTS AND DISCUSSION : Acetyl hexapeptide-1 prevents UV-induced genotoxic effects in primary human melanocytes - Acetyl hexapeptide-1 protects human keratinocytes from UVA-induced oxidative damage to DNA - Acetyl hexapeptide-1 protects human skin ex vivo from UV-induced pyrimidine dimers and apoptosis - Anti-erythemal properties of acetyl hexapeptide-1, a clinical study |
Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=14957 |
in IFSCC MAGAZINE > Vol. 15, N° 2 (04-05-06/2012) . - p. 89-95
[article]
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