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Reproducible method for assessing the effects of blue light using in vitro human skin tissues / Rishabh Kala in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 1 (02/2023)
[article]
Titre : Reproducible method for assessing the effects of blue light using in vitro human skin tissues Type de document : document électronique Auteurs : Rishabh Kala, Auteur ; Nicole Heiberger, Auteur ; Heather Mallin, Auteur ; Stephanie Wheeler, Auteur ; Anna Langerveld, Auteur Année de publication : 2023 Article en page(s) : p. 95-107 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Elisa, Test Le test ELISA est une méthode de laboratoire qualifiée d'immuno-enzymatique. Il s'agit d'un test immunologique, car il a pour principal objectif de mettre en évidence la présence d'anticorps ou d'antigènes spécifiques à une pathologie, dans un échantillon de sang. Cet examen utilise une protéine appelée enzyme, qui va se fixer à certains constituants spécifiques de la maladie, et par repérage et quantification de cette enzyme, la maladie pourra être affirmée, ainsi que son intensité évaluée.
Expression génique
Lumière bleue
Peau -- Inflammation
Peau -- Maladies
Stress oxydatif
Vieillissement cutanéIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Introduction : High-intensity visible light (HEV), also referred to as blue light, has a wavelength of 400–500 nm and accounts for approximately one-third of the visible light. Blue light is also emitted from electronic devices and artificial indoor lighting. Studies have shown that exposure of human skin cells to light emitted from electronic devices, even as short as 1 h, can cause an increase in reactive oxygen species (ROS), apoptosis and necrosis. Despite comprising a significant portion of the light spectrum, the effects of HEV light have not been studied as extensively. This is in part due to a lack of suitable in vitro testing methods. This work was conducted in order to develop a reproducible testing method for assessing the effects of blue light on the skin.
- Methods : Testing was performed using a full thickness, 3D in vitro skin tissue model. Different exposure protocols were tested to (1) determine the biological effects of blue light on the skin and (2) to identify an appropriate exposure for routine testing of cosmetic materials that may protect the skin from blue light damage. Gene expression and protein biomarkers were measured using qPCR, ELISA and immunohistochemical (IHC) methods.
- Results : Our work demonstrates that daily exposure to blue light produced dose-and-time-dependent changes in biomarkers associated with skin damage. Exposure to blue light for 6 h for 5 consecutive days (total intensity of 30 J/cm2) increased the expression of genes that regulate inflammation and oxidative stress pathways and decreased the expression of genes that maintain skin barrier and tissue integrity. Exposure to blue light significantly increased protein biomarkers associated with ageing, inflammation and tissue damage. IHC staining confirmed changes in collagen, filaggrin and NQO1 protein expression. Treatment with ascorbic acid inhibited the effects of blue light, demonstrating a role in protection from blue light.
- Conclusion : Our results showed that consistent blue light exposure produced skin damage via alterations in biological pathways that are associated with skin ageing. This work provides a new, reproducible in vitro testing method for assessing the effects of blue light on human skin using gene expression, protein ELISA and IHC staining.Note de contenu : - MATERIALS AND METHODS : In vitro skin tissues - Treatment of skin tissues - RNA isolation and quantitative reverse Transcription-polymerase chain reaction (qRT-PCR) analysis - Enzyme-linked
immunosorbent (ELISA) protein assays - Immunohistochemistry (IHC) staining
- RESULTS AND DISCUSSION : Blue light produced dose-dependent
changes in gene expression - Blue light produced oxidative DNA damage - Blue light exposure caused an increase in skin ageing and MMP1 activity - Blue light exposure caused an increase in
pro-inflammatory cytokine expression - Vitamin C prevents blue light damage
- Table 1 : Statistically significant (unpaired t test, p < 0.05, N = 4) changes in gene expression are shown in Table 1 for each group vs the unexposed groupDOI : https://doi.org/10.1111/ics.12821 En ligne : https://drive.google.com/file/d/15yOdVPKCyBpYYMPGCXgl8r5MER1WHETA/view?usp=share [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38823
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 1 (02/2023) . - p. 95-107[article]
[article]
Titre : Seeing clearly : Blue light absorbers help an optical clear adhesive block light transmittance Type de document : texte imprimé Auteurs : Sylvia Hung, Auteur ; Miles Hsieh, Auteur ; Ray Sung, Auteur ; Y. H. Huang, Auteur Année de publication : 2019 Article en page(s) : p. 12-16 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Absorbants et adsorbants
Adhésifs dans les équipements électriques/électroniques
Couleur
Essais accélérés (technologie)
Lumière -- Absorption
Lumière bleue
TransmittanceLa transmittance, en général, est le rapport caractérisant la transmission d'une grandeur dans un système. Elle se calcule par le rapport entre la grandeur en entrée et en sortie.
En optique, la transmittance d'un matériau ou d'un filtre est la fraction du flux lumineux le traversant. Elle est également nommée facteur de transmission mais aussi transparence dans le domaine de la photographie.Index. décimale : 668.3 Adhésifs et produits semblables Résumé : Digital personal devices such as smartphones and tablets have become necessities in modern life. However, for image sharpness, most digital devices emit blue light of 380-500 nm in the light spectrum, with higher energy. In some studies, macular degradation has been shown to result from long-term exposure to these wavelengths.
In daily life, blue light cut off is needed for health reasons in many situations. For instance, yellow dye is often introduced in eye glasses, goggles, liquid crystal displays (LCDs) and light emitting diodes (LEDs). These additives can be incorporated in optical adhesives for the same purpose. Therefore, optical adhesives are also widely used in the electro-optical, green energy and consumer markets. The adhesives can be found in acrylic, unsaturated polyester, polyurethane, and other resin systems. This article compares a blue light absorber and a yellow solvent dye in optical adhesive applications.Note de contenu : - EXPERIMENT : Material - Methods - Examination
- RESULTS : Transmittance - Accelerated weathering test - Color
- Fig. 1 : UV-vis spectra of BL
- Fig. 2 : UV-vis spectra of X and Y
- Fig. 3 : UV-vis spectra of X
- Fig. 4 : CIE L* a* b* data of BL
- Fig. 5 : Appearance of X and BL
- Table 1 : Solid content in sample
- Table 2 : Transmittance data of BL
- Table 3 : CIE L* a* b* data of XEn ligne : https://www.adhesivesmag.com/articles/96796-using-blue-light-absorbers-to-see-op [...] Format de la ressource électronique : Web Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=32187
in ADHESIVES & SEALANTS INDUSTRY (ASI) > Vol. 26, N° 3 (03/2019) . - p. 12-16[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 20975 - Périodique Bibliothèque principale Documentaires Disponible Strengthening natural protection against light / Sonia Léglise in PERSONAL CARE EUROPE, Vol. 14, N° 2 (04/2020)
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Titre : Strengthening natural protection against light Type de document : texte imprimé Auteurs : Sonia Léglise, Auteur Année de publication : 2020 Article en page(s) : p. 174-176 Langues : Anglais (eng) Catégories : ADN mitochondrial
Antiâge
Biomolécules actives
Collagène
Cosmétiques
Dermo-cosmétologie
Elastine
Hyaluronique, acideL'acide hyaluronique est un type de polysaccharide (plus précisément une glycosaminoglycane) non fixé à une protéine centrale et largement réparti parmi les tissus conjonctifs, épithéliaux et nerveux animaux.
Il se trouve notamment dans l'humeur vitrée et le liquide synovial. Il est l'un des principaux composants de la matrice extracellulaire ainsi que de certaines mucoprotéines lorsqu'il est associé à une fraction protéique.
Depuis les années 1990, il est très utilisé dans divers dispositifs médicaux, médicamenteux et cosmétiques (présenté dans ce dernier cas comme "antistatique, humectant, hydratant, conditionneur cutané, anti-âge, etc."), bénéficiant d'un effet de mode grandement soutenu par une large publicité commerciale.
Contribuant de façon significative à la prolifération et à la migration des cellules, l'acide hyaluronique est aussi impliqué dans la progression de certains cancers. (Wikipedia)
Lumière artificielle -- Effets physiologiques
Lumière bleue
Nitrique, Acide
Peau -- Effets du rayonnement solaire
Peau -- Soins et hygiène
Protection cutanéeIndex. décimale : 668.5 Parfums et cosmétiques Résumé : Naolys has creted a new complex of Indian jasmine cells enriched with y-mangosteen to develop a unique biological protection for the skin.
Jasmin is well-known oriental flower, famed for its scent and its sacred status in Asian religions ; it opens at the end of the day mangosteen is an extract from the fruit of the Asian medicine, it has come to be recognised in the west for its antioxidant properties.
Enriched by the synergy with the y-mangosteen, the plant cells increase the bioavailabiliaty of all the active ingredients that they deliver to the skin.Note de contenu : - A NEW ACTIVE INGREDIENT
- HOX IT WORKS : Reinforced biological protection - Reduction of damage caused by light
- CLINICAL TEST RESULTS : Decrease in signs of ageing after 28 days - two applications/day
- IN VITRO TEST RESULTS : Protection the heart of the cells : UV rays - Blue light - Infrared light - Study of abasic ells - Study of mitochondrial DNA
- PROTECTING THE FIBRES AND THE POLYSACCHARIDES IN THE DERMIS : THE EXTRA-CELLULAR MATRIX
- STUDY OF KEY COMPONENTS IN THE EXTRA-CELLULAR MATRIX : Hyaluronic acid - Collagen - Elastin
- REDUCING VASOLIDATION : CUTANEOUS MICROCIRCULATION : Study of nitric oxide the primary EDRF
- Fig. 1 : Solar rays and their effects on skin
- Fig. 2 : Decrease in signs of ageing after 28 days
- Fig. 3 : Study of mitochondrial DNA
- Fig. 4 : Study of collagens
- Fig. 5 : Study of nitric oxide exposed to three key types of solar radiationEn ligne : https://drive.google.com/file/d/1ggCd5jAP5KnReBfWGVJg2p9Dv1t9F73f/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=33971
in PERSONAL CARE EUROPE > Vol. 14, N° 2 (04/2020) . - p. 174-176[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 21621 - Périodique Bibliothèque principale Documentaires Disponible The protective effect of a novel sunscreen against blue light Background and objective / Veronique Francois-Newton in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 44, N° 4 (08/2022)
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Titre : The protective effect of a novel sunscreen against blue light Background and objective Type de document : document électronique Auteurs : Veronique Francois-Newton, Auteur ; Vimi Lutchmanen Kolanthan, Auteur ; Madiiha Bibi Mandary, Auteur ; Evans Georges Philibert, Auteur ; Vitisha Soobramaney, Auteur ; Gitanjali Petkar, Auteur ; Bibi Nusayha Sokeechand, Auteur ; Muzzammil Hosenally, Auteur ; Andrea Cavagnino, Auteur ; Martin Andres Baraibar, Auteur ; Sue Phay Ng, Auteur Année de publication : 2022 Article en page(s) : p. 464-476 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Carbonylation
Dermo-cosmétologie
Lumière bleue
MélasmaLe mélasma (également dénommé chloasme ou chloasma) est une affection bénigne de la peau se présentant sous la forme de taches hyperpigmentées apparaissant sur les zones exposées au soleil, surtout au niveau du visage, du décolleté et du cou.
Le mélasma atteint principalement les femmes, le plus souvent lors de la grossesse, donnant ce que l'on appelle communément le masque de grossesse, mais il peut apparaître en dehors de celle-ci.
Les causes et les facteurs favorisants du mélasma sont essentiellement des facteurs de prédisposition génétique, l’exposition au soleil et aux œstrogènes (lors de la grossesse ou au cours d'une contraception).
Peau -- Soins et hygiène
Pigmentation de la peau
Produits antisolaires
ProtéinesIndex. décimale : 668.5 Parfums et cosmétiques Résumé : - Background and objective : Premature skin ageing, and skin hyperpigmentation are influenced by exogenous factors, such as ultraviolet radiation and blue light. In this study, we assess the protective effect of a sunscreen (TDF® Blu Voile Sunscreen) in protecting the skin against the harmful effects of blue light irradiation in vivo and through the in situ quantitative and qualitative evaluation of protein carbonylation in human skin explants.
- Methodology : The protective effect of the test product against blue light was first evaluated ex vivo on human skin explants. The treated and non-treated explants were exposed to 14 J/cm2 of blue light 460 nm following which the protein carbonylation was evaluated by in situ epifluorescence imaging and separation by high-resolution gel electrophoresis. To determine whether the test product could also protect against the immediate and persistent pigmenting effect of blue light, two randomized in vivo studies were conducted, which included respectively 17 subjects with a skin phototype of IV and V (Fitzpatrick classification) and 22 subjects with a skin phototype of IV, V, and VI (Fitzpatrick classification). The duration of the study for each subject was 2 days (D1 and D2) for immediate observations and 5 days (D1–D5) for persistent observations. Specific zones on the subjects' back were either left non-treated or treated with the test product and were then exposed to a unique dose of blue light 415 nm. The onset of pigmentation between the treated and exposed zones was then assessed relative to the non-exposed treated zone through colorimetric measurements of the Individual Typology Angle (ITAo).
- Results : Human skin explants treated with test product showed significantly lower levels of accumulated carbonylated proteins, with a protection of 82%, following exposure to blue light 460 nm. Findings of the in vivo studies also indicated that the test product presented significantly better protective efficacy against immediate and persistent pigmentation induced by blue light 415 nm.
- Conclusion : Hence, it can be concluded that the test product can protect against the oxidative stress as well as the immediate and persistent pigmentation induced by blue light.Note de contenu : - MATERIALS AND METHODS : Test compounds - Blue lights doses - Ex vivo protein carbonylation study - Human skin explants preparation - In situ visualization and semi-quantification of carbonylated proteins - Extraction and absolute quantification of carbonylated proteins - Statistical analysis - In vivo clinical study with volunteers - Evaluation against the immediate pigmenting effect of blue light - Evaluation against the persistent pigmenting effect of blue light - Colorimetric measurement - Statistical analysis
- RESULTS : Prevention against accumulation of carbonylated proteins in human skin explants - Protection against the immediate pigmenting effect of blue light - Illustrative photographs of immediate
pigmentation - Protection against the persistent pigmenting effect of blue light
- Table 1 : Experimental group and design
- Table 2 : The study flow of the in vivo trial for immediate pigmentation effect of blue light
- Table 3 : The study flow of the in vivo trials for persistent pigmentation effect of blue lightDOI : https://doi.org/10.1111/ics.12794 En ligne : https://drive.google.com/file/d/1oEDQa-wCBoOiHBVvptrg1X1eEg6VkisC/view?usp=shari [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38146
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 4 (08/2022) . - p. 464-476[article]Unlocking UV benefits and claims beyond SPF / Amy Huang in GLOBAL PERSONAL CARE, Vol. 24, N° 9 (10/2023)
[article]
Titre : Unlocking UV benefits and claims beyond SPF Type de document : texte imprimé Auteurs : Amy Huang, Auteur ; Rolf Schütz, Auteur ; Jochen Klock, Auteur Année de publication : 2023 Article en page(s) : p. 37-40 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cancer de la peau -- Prévention
Dermo-cosmétologie
Immunité
Lumière bleue
Peau -- Soins et hygiène
Photovieillissement (dermatologie)
Pigmentation, Troubles de la
Produits antisolaires
Protection cutanée
Rayonnement ultraviolet -- Effets physiologiques
Vieillissement cutanéIndex. décimale : 668.5 Parfums et cosmétiques Résumé : Despite the mounting evidence that even minimal exposure to ultraviolet (UV) radiation can lead to visible signs of skin aging, and the growing concerns among consumers regarding the impact of sun exposure on facial skin, the regular use of facial UV protection remains relatively low. One contributing factor to this trend is the prevailing belief that sun protection is only necessary when engaging in activities like going to the beach, where the primary concern is avoiding sunburn. However, it is important to recognize that while UV filters effectively shield the skin from sunburn, they also offer a broader range of benefits for skincare. To raise awareness about these advantages, DSM-Firmenich conducted an extensive review of scientific studies exploring the role of UV filters in five key areas: prevention of photoaging, hyperpigmentation prevention, protection against blue light, defence against the risk of skin cancer, and support for skin barrier immunity. In this article, we present a summary of the main findings in each of these areas Note de contenu : - Benefit 1 : daily use of UV filters can help prevent photoageing
- Benefit 2 : UV filters can help reduce the effects of pigmentation disorders
- Benefit 3 : UV filters offer protection against blue light
- Benefit 4 : UV filters can help reduce the risk of skin cancer
- Benefit 5 : UV filters can support the immune functionEn ligne : https://drive.google.com/file/d/1UnMavPF3M0VGdw8QkPKLzXb6a34XQoyM/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40349
in GLOBAL PERSONAL CARE > Vol. 24, N° 9 (10/2023) . - p. 37-40[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 24226 - Périodique Bibliothèque principale Documentaires Disponible UV filters - Exploring the benefits beyond sunburn protection / Amy Huang in SOFW JOURNAL, Vol. 148, N° 9 (09/2022)
PermalinkWell protected against multi-light and pollution / Solène Mine in SOFW JOURNAL, Vol. 145, N° 11 (11/2019)
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