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Auteur Wolfgang F. Pittermann
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Gematria - Düsseldorf - Germany
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Experimentlly induced epidermal barrier perturbation : measurement of transepidermal water loss (TEWL) using the perfused bovine udder skin (BUS) model / Wolfgang F. Pittermann in IFSCC MAGAZINE, Vol. 3, N° 1 (01-02-03/2000)
[article]
Titre : Experimentlly induced epidermal barrier perturbation : measurement of transepidermal water loss (TEWL) using the perfused bovine udder skin (BUS) model Type de document : texte imprimé Auteurs : Wolfgang F. Pittermann, Auteur ; Th. Gassenmeir, Auteur ; S. Nieveler, Auteur ; Thomas Förster, Auteur ; M. Kietzmann, Auteur Année de publication : 2000 Article en page(s) : p. 29-32 Langues : Anglais (eng) Tags : 'Bovine Under Skin (BUS) model' 'Natural skin barrier' TEWL 'Oleic acid' Stripping Irritation Penetration Index. décimale : 668.5 Parfums et cosmétiques Résumé : This paper describes the measurement of the TEWL under in vitro conditions using the isolated perfused Bovine Under Skin (BUS) model. After perturbation of the skin barrier by application of pure oleic acid or graduation stripping an increase in TEWL as expected was determined. The penetration of pure oleic acid into the stratum corneum was proven. No cytotoxic or irritant effects were observed after an exposure period up to five hours. Note de contenu : Bibliogr. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10845
in IFSCC MAGAZINE > Vol. 3, N° 1 (01-02-03/2000) . - p. 29-32[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 003870 - Périodique Bibliothèque principale Documentaires Disponible New methods for testing skin protection against Noxae using the Bovine Udder Skin (BUS) / Katinka Jung in IFSCC MAGAZINE, Vol. 15, N° 4 (10-11-12/2012)
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Titre : New methods for testing skin protection against Noxae using the Bovine Udder Skin (BUS) Type de document : texte imprimé Auteurs : Katinka Jung, Auteur ; Wolfgang F. Pittermann, Auteur ; Marietta Seifert, Auteur ; Thomas Herrling, Auteur Année de publication : 2012 Article en page(s) : p. 265-270 Note générale : Biobliogr. Langues : Anglais (eng) Catégories : Bovins
Etude in vivo
Expérimentation animale
Mamelle
Peau -- Inflammation
Produits chimiques -- Toxicologie
Protection cutanée
Radicaux libres (chimie)
Spectroscopie de résonance paramagnétique électronique
Tests de toxicité
Toxicologie cellulaireIndex. décimale : 668.5 Parfums et cosmétiques Résumé : Quantitative and qualitative determination of skin irritation and skin protection through market formulations using an in vitro skin model, the perfused bovine udder (BUS) and different analytical methods, was the objective of the present research. Various noxae that are percutaneously absorbed cause skin irritation and damage through different mechanisms. Cell vitality, signs of inflammation, and the presence of inducible free radicals among the analytical parameters that enable quantification of skin damage. In this study cytotoxic effects were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and tissue levels of the pre-inflammatory mediator prostaglandin E2 in treated/untreated whole skin biopsies were assessed using an enzyme immunoassay. The inducibility of free radicals was determined by electron resonance spectroscopy techniques. Noxae (standardized chemicals and products) like detergents, coolants, toluene, and n-octane caused reduced cell vitality and metabolic activity, inflammation as evidenced by the presence of prostaglandins, and enhanced values in the inducible free radical status in in vitro bovine udder skin. The effect of the noxae is dose-dependent and permeation time-dependent, allowing construction of dose-effect curves. The induced cytotoxicity and free radical status show a positive correlation, while the inflammatory actions analyzed by the prostaglandin E2 tissue concentration do not.
As a consequence, the skin compatibility of noxae and performance of skin protection formulations can be evaluated by the measurement of different cell physiological and biophysical parameters at the very early stages of skin damage.Note de contenu : - Chemicals
- Skin protective formulation A
- Skin protective formulation B
- BUS model
- UV irradiation
- ESR spectrometer
- Determination of the radical skin status factor RSFPermalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=16885
in IFSCC MAGAZINE > Vol. 15, N° 4 (10-11-12/2012) . - p. 265-270[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 14431 - Périodique Bibliothèque principale Documentaires Disponible Proceedings of a workshop on isolated perfused organs (Hamburg, 1998) / Wolfgang F. Pittermann in IFSCC MAGAZINE, Vol. 3, N° 2 (04-05-06/2000)
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Titre : Proceedings of a workshop on isolated perfused organs (Hamburg, 1998) Type de document : texte imprimé Auteurs : Wolfgang F. Pittermann, Auteur Année de publication : 2000 Article en page(s) : p. 38-39 Langues : Anglais (eng) Index. décimale : 668.5 Parfums et cosmétiques Résumé : During the last decade a wide range of isolated organ systems have been developed in biomedical research. Sophisticated equipment, increasing manual skill, routine and standardisation have led to an increase of validity and precision of experimental results under circumstances that are virtually "true to life". Today these systems contribute a considerable amount of in-vitro knowledge to the scientific community.
Promoting animal welfare on all fields of Laboratory Animal Science (GV-SOLAS) has decided to organise a workshop during the 36th Scientific Meeting in Hamburg (Sept 1998) dealing with the development and definition of perfused isolated organ systems.Note de contenu : - Advantages and limitations of perfused isolated organs Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10837
in IFSCC MAGAZINE > Vol. 3, N° 2 (04-05-06/2000) . - p. 38-39[article]Réservation
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Code-barres Cote Support Localisation Section Disponibilité 004648 - Périodique Bibliothèque principale Documentaires Disponible