[article]
| Titre : |
New methods for testing skin protection against Noxae using the Bovine Udder Skin (BUS) |
| Type de document : |
texte imprimé |
| Auteurs : |
Katinka Jung, Auteur ; Wolfgang F. Pittermann, Auteur ; Marietta Seifert, Auteur ; Thomas Herrling, Auteur |
| Année de publication : |
2012 |
| Article en page(s) : |
p. 265-270 |
| Note générale : |
Biobliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Bovins
Etude in vivo
Expérimentation animale
Mamelle
Peau -- Inflammation
Produits chimiques -- Toxicologie
Protection cutanée
Radicaux libres (chimie)
Spectroscopie de résonance paramagnétique électronique
Tests de toxicité
Toxicologie cellulaire
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
Quantitative and qualitative determination of skin irritation and skin protection through market formulations using an in vitro skin model, the perfused bovine udder (BUS) and different analytical methods, was the objective of the present research. Various noxae that are percutaneously absorbed cause skin irritation and damage through different mechanisms. Cell vitality, signs of inflammation, and the presence of inducible free radicals among the analytical parameters that enable quantification of skin damage. In this study cytotoxic effects were determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) assay, and tissue levels of the pre-inflammatory mediator prostaglandin E2 in treated/untreated whole skin biopsies were assessed using an enzyme immunoassay. The inducibility of free radicals was determined by electron resonance spectroscopy techniques. Noxae (standardized chemicals and products) like detergents, coolants, toluene, and n-octane caused reduced cell vitality and metabolic activity, inflammation as evidenced by the presence of prostaglandins, and enhanced values in the inducible free radical status in in vitro bovine udder skin. The effect of the noxae is dose-dependent and permeation time-dependent, allowing construction of dose-effect curves. The induced cytotoxicity and free radical status show a positive correlation, while the inflammatory actions analyzed by the prostaglandin E2 tissue concentration do not.
As a consequence, the skin compatibility of noxae and performance of skin protection formulations can be evaluated by the measurement of different cell physiological and biophysical parameters at the very early stages of skin damage. |
| Note de contenu : |
- Chemicals
- Skin protective formulation A
- Skin protective formulation B
- BUS model
- UV irradiation
- ESR spectrometer
- Determination of the radical skin status factor RSF |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=16885 |
in IFSCC MAGAZINE > Vol. 15, N° 4 (10-11-12/2012) . - p. 265-270
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