[article]
| Titre : |
Molecular basis of retinol anti-ageing properties in naturally aged human skin in vivo |
| Type de document : |
texte imprimé |
| Année de publication : |
2017 |
| Article en page(s) : |
p. 56-65 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Antiâge
Etude in vivo
Matrice extracellulaire
Personnes âgées
Rétinoïdes
Vitamine A
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
Objective : Retinoic acid has been shown to improve the aged-appearing skin. However, less is known about the anti-ageing effects of retinol (ROL, vitamin A), a precursor of retinoic acid, in aged human skin in vivo. This study aimed to investigate the molecular basis of ROL anti-ageing properties in naturally aged human skin in vivo.
Methods : Sun-protected buttock skin (76 ± 6 years old, n = 12) was topically treated with 0.4% ROL and its vehicle for 7 days. The effects of topical ROL on skin epidermis and dermis were evaluated by immunohistochemistry, in situ hybridization, Northern analysis, real-time RT-PCR and Western analysis. Collagen fibrils nanoscale structure and surface topology were analysed by atomic force microscopy.
Results : Topical ROL shows remarkable anti-ageing effects through three major types of skin cells : epidermal keratinocytes, dermal endothelial cells and fibroblasts. Topical ROL significantly increased epidermal thickness by stimulating keratinocytes proliferation and upregulation of c-Jun transcription factor. In addition to epidermal changes, topical ROL significantly improved dermal extracellular matrix (ECM) microenvironment; increasing dermal vascularity by stimulating endothelial cells proliferation and ECM production (type I collagen, fibronectin and elastin) by activating dermal fibroblasts. Topical ROL also stimulates TGF-?/CTGF pathway, the major regulator of ECM homeostasis, and thus enriched the deposition of ECM in aged human skin in vivo. 0.4% topical ROL achieved similar results as seen with topical retinoic acid, the biologically active form of ROL, without causing noticeable signs of retinoid side effects.
Conclusion : 0.4% topical ROL shows remarkable anti-ageing effects through improvement of the homeostasis of epidermis and dermis by stimulating the proliferation of keratinocytes and endothelial cells, and activating dermal fibroblasts. These data provide evidence that 0.4% topical ROL is a promising and safe treatment to improve the naturally aged human skin. |
| Note de contenu : |
- MATERIALS AND METHODS : Procurement of human tissue samples and retinol treatment in human skin in vivo - Immunohistology - RNA isolation and Northern blot analysis - In situ hybridization - RNA isolation and quantitative real-time RT-PCR - Western blot analysis - Atomic force microscopy (AFM) imaging - Statistical analysis
- RESULTS : Topical ROL increases epidermal thickness and dermal vascularity by proliferation of epidermal keratinocytes and dermal endothelial cells, respectively, in aged humak skin in vivo - ROL improves dermal ECM microenvironment by stimulating the expression of type I collagen, fibronectin and tropoelastin in aged human skin in vivo - Elevated epidermal-specific c-jun transcription factor by topical ROL in aged human skin in vivo - Elevated TGF-?/CTGF pathway by topical ROL in aged human skin in vivo - Topical ROL increases the deposition of mature collagen in aged human skin in vivo |
| DOI : |
10.1111/ics.12348 |
| En ligne : |
https://drive.google.com/file/d/1BYADKKYvLJrcMJVsWZqEe7Av_9vNk_5x/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=27843 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 39, N° 1 (02/2017) . - p. 56-65
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