| Titre : |
HPLC-TBARS-EVSC (high-performance liquid chromatography-thiobarbituric acid reactive substances–ex vivo stratum corneum) protocol : Selection of the subjects and approach to present the results |
| Type de document : |
texte imprimé |
| Auteurs : |
Gabriela de Argollo Marques, Auteur ; Camila Faustino Hiraishi, Auteur ; Pedro Ivo de Souza Macedo, Auteur ; Claudineia Aparecida Sales de Oliveira Pinto, Auteur ; Joao Gregorio, Auteur ; Catarina Rosado, Auteur ; Maria Valéria Robles Velasco, Auteur ; André Rolim Baby, Auteur |
| Année de publication : |
2023 |
| Article en page(s) : |
p. 647-654 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Chimie analytique
Dermo-cosmétologie
Différences d'âge
Différences entre sexes
Peau -- Physiologie
Phototype (dermatologie)En dermatologie, le phototype permet de classer les individus selon la réaction de leur peau lors d'une exposition solaire.
Statistiques
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : Nowadays, it is recognized the need for improved safety and efficacy protocols to evaluate the human stratum corneum (SC) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies. The aim of our research work was to streamline the HPLC-TBARS-EVSC (high-performance liquid chromatography–thiobarbituric acid reactive substances–ex vivo stratum corneum) methodology, by exploring the results of a group of 18 subjects.
- Methods : The study included nine women and nine men aged between 19 and 57 years old with phototypes from II to V. Sites in the forearm of each volunteer were randomly delimited, and the SC was collected by tape stripping. HPLC was used to quantify the MDA-TBA2 (malondialdehyde–thiobarbituric acid) adduct from the tape-stripped SC, irradiated and not by an ultraviolet (UV) simulator chamber.
- Results : Observing the findings of our present investigation, and the statistical approach applied, the use of the ratio between the treatment site and control would be an adequate strategy to better discriminate and evaluate the results. Additionally, an optimal selection of the volunteers to respond specifically to the purpose of the ex vivo assay also can be considered advantageous.
- Conclusions : It seemed that in future studies focusing on the impact of SC UV-induced lipid peroxidation, determined by the HPLC-TBARS-EVSC, the most suitable subjects are females aged less than 35 years old, with phototype II. |
| Note de contenu : |
- Legal and ethical concerns, and subjects' selection
- Tape-stripping procedure and sample preparation
- HPLC-TBARS-EVSC analytical protocol (chromatographic conditions)
- Mathematical and statistical approach
- Table 1 : Sex, age (years), phototype and area under the curve of the MDA-TBA2 adduct for the nonirradiated and the irradiated stratum corneum samples obtained from the subjects |
| DOI : |
https://doi.org/10.1111/ics.12874 |
| En ligne : |
https://drive.google.com/file/d/1Zpdg2oMsqdLWGRx9WTq-__me3DqJf3fk/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40091 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 5 (10/2023) . - p. 647-654
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