A controlled forearm washing ex vivo method for assessing the impact of personal cleansing products on skin's acid mantle properties and antimicrobial defence against transient bacteria / Feng Yue in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 3 (06/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 3 (06/2023) . - p. 354-361 Titre : A controlled forearm washing ex vivo method for assessing the impact of personal cleansing products on skin's acid mantle properties and antimicrobial defence against transient bacteria Type de document : document électronique Auteurs : Feng Yue, Auteur ; Betty Yu, Auteur ; Lesheng Zhang, Auteur ; Amy Yu, Auteur ; Caroline Wang, Auteur ; Zigang Xu, Auteur ; Karl We, Auteur Année de publication : 2023 Article en page(s) : p. 354-361 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Dermo-cosmétologie Escherichia coli Film hydrolipidique (dermatologie) Le film hydrolipidique est un film protecteur recouvrant la totalité de la surface de l'épiderme humain. C'est une émulsion complexe formée de substances provenant de sécrétions (sueur, sébum...) et d'agrégats cellulaires kératinisés ou morts. Son pH est légèrement acide. Ce film est à la base de la qualité de l'aspect extérieur de la peau puisqu'il la protège de plusieurs façons : - Il participe à la constitution de la barrière cutanée (avec la couche cornée) qui s'oppose à la pénétration de substances étrangères, - Il régule l'hydratation en maintenant les substances hygroscopiques (qui ont des affinités avec l'eau) dans les cellules cornées, - Il lutte contre les agressions microbiennes en autorisant toutefois le développement de la flore normale. (Wikipedia) Peau -- Soins et hygiène pH Produits nettoyants Staphylocoques Surfaces antimicrobiennes Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : The objective of this study was to develop a controlled forearm washing ex vivo method for assessing the impact of personal cleansing products on skin's acid mantle properties and antimicrobial defence against transient bacteria. - Methods : We developed a controlled forearm washing ex vivo method (ex vivo NET method) to compare the impact of two representative personal cleansing products on skin's acid mantle properties and antimicrobial defence against transient bacteria: one was a low-pH skin cleanser, and the other was high-pH soap cleanser. Skin pH was measured at baseline and 4 h after the product application. Concurrently, D-squame tape stripping procedure was followed to sample the stratum corneum surface layers. Then, two selected transient bacteria, Staphylococcus aureus and Escherichia coli, were inoculated onto the D-squame tapes and incubated under controlled conditions, respectively. The residual bacteria counts can provide an objective measure of skin's acid mantle properties against transient bacteria. Results from the ex vivo NET method were compared with the traditional in vivo cup-scrub RET method. - Results : The skin pH was significantly lower 4 h after washing the forearm with the low-pH cleanser versus the high-pH soap, consistent with literatures. Interestingly, the skin surface washed by the low-pH cleanser showed significantly higher hostility against representative transient bacteria as demonstrated by the lower counts of S. aureus by 1.09 log and E. coli by 0.6 log versus the high-pH soap based on the ex vivo NET method. Results from the ex vivo NET method were further supported by the traditional in vivo RET method which also showed the skin washed by the low-pH cleanser had significantly lower counts of S. aureus and E. coli versus the high-pH soap. - Conclusions : The skin's acid mantle properties and antimicrobial defence can be directly impacted by the personal cleansing products. The low-pH skin cleanser works better than the high-pH soap for supporting skin's acid mantle properties and antimicrobial defence against transient bacteria. Results from the ex vivo NET method are consistent with the in vivo RET method. It is important that the ex vivo NET method offers many advantages since it is quicker to run with higher throughput and has better safety without the constraint of inoculating harmful microorganisms onto the human subjects. Note de contenu : - Compositions of high-pH and low-pH skin cleansers - Controlled forearm washing ex vivo NET method - In vivo Cup-Scrub RET method - Statistical analysis - Table 1 : Skin pH at baseline and 4 h after product application comparing the high pH soap (product A, pH = 11.5) and the low-pH synthetic cleanser (product B, pH-4.55). The skin-pH values were significantly lower by 0.38 units (p < 0.05) at 4 h after using product B versus product A. - Table 2 : Log(CFU) of Staphylococcus aureus and Escherichia coli from in vivo cup-scrub RET method. The counts of S. aureus and E. coli were significantly lower by 0.86 log and 2.15 log, respectively, after using the low-pH cleanser (product B at pH = 4.55) versus the high-pH soap cleanser (product A at pH = 11.5). DOI : https://doi.org/10.1111/ics.12840 En ligne : https://drive.google.com/file/d/1NRJU0CrKlcWuEOs1wRGFNSTmGJQcv0ZG/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39566 [article]

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