HPLC-TBARS-EVSC (high-performance liquid chromatography-thiobarbituric acid reactive substances–ex vivo stratum corneum) protocol : Selection of the subjects and approach to present the results / Gabriela de Argollo Marques in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 45, N° 5 (10/2023)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 45, N° 5 (10/2023) . - p. 647-654 Titre : HPLC-TBARS-EVSC (high-performance liquid chromatography-thiobarbituric acid reactive substances–ex vivo stratum corneum) protocol : Selection of the subjects and approach to present the results Type de document : texte imprimé Auteurs : Gabriela de Argollo Marques, Auteur ; Camila Faustino Hiraishi, Auteur ; Pedro Ivo de Souza Macedo, Auteur ; Claudineia Aparecida Sales de Oliveira Pinto, Auteur ; Joao Gregorio, Auteur ; Catarina Rosado, Auteur ; Maria Valéria Robles Velasco, Auteur ; André Rolim Baby, Auteur Année de publication : 2023 Article en page(s) : p. 647-654 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Chimie analytique Dermo-cosmétologie Différences d'âge Différences entre sexes Peau -- Physiologie Phototype (dermatologie) En dermatologie, le phototype permet de classer les individus selon la réaction de leur peau lors d'une exposition solaire. Statistiques Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Nowadays, it is recognized the need for improved safety and efficacy protocols to evaluate the human stratum corneum (SC) and its interaction with topical and cosmetic formulations by minimally or non-invasive methodologies. The aim of our research work was to streamline the HPLC-TBARS-EVSC (high-performance liquid chromatography–thiobarbituric acid reactive substances–ex vivo stratum corneum) methodology, by exploring the results of a group of 18 subjects. - Methods : The study included nine women and nine men aged between 19 and 57 years old with phototypes from II to V. Sites in the forearm of each volunteer were randomly delimited, and the SC was collected by tape stripping. HPLC was used to quantify the MDA-TBA2 (malondialdehyde–thiobarbituric acid) adduct from the tape-stripped SC, irradiated and not by an ultraviolet (UV) simulator chamber. - Results : Observing the findings of our present investigation, and the statistical approach applied, the use of the ratio between the treatment site and control would be an adequate strategy to better discriminate and evaluate the results. Additionally, an optimal selection of the volunteers to respond specifically to the purpose of the ex vivo assay also can be considered advantageous. - Conclusions : It seemed that in future studies focusing on the impact of SC UV-induced lipid peroxidation, determined by the HPLC-TBARS-EVSC, the most suitable subjects are females aged less than 35 years old, with phototype II. Note de contenu : - Legal and ethical concerns, and subjects' selection - Tape-stripping procedure and sample preparation - HPLC-TBARS-EVSC analytical protocol (chromatographic conditions) - Mathematical and statistical approach - Table 1 : Sex, age (years), phototype and area under the curve of the MDA-TBA2 adduct for the nonirradiated and the irradiated stratum corneum samples obtained from the subjects DOI : https://doi.org/10.1111/ics.12874 En ligne : https://drive.google.com/file/d/1Zpdg2oMsqdLWGRx9WTq-__me3DqJf3fk/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40091 [article]

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Impact of Portuguese propolis on keratinocyte proliferation, migration and ROS protection: Significance for applications in skin products / Nuno Saraiva in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 44, N° 3 (06/2022)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 3 (06/2022) . - p. 333-342 Titre : Impact of Portuguese propolis on keratinocyte proliferation, migration and ROS protection: Significance for applications in skin products Type de document : document électronique Auteurs : Nuno Saraiva, Auteur ; Marisa Nicolai, Auteur ; Marta Martins, Auteur ; Nuno Almeida, Auteur ; Matteo Gusmini, Auteur ; Elisabete Muchagato Maurício, Auteur ; Maria Paula Duarte, Auteur ; Margarida Gonçalves, Auteur ; André Rolim Baby, Auteur ; Ana Fernandes, Auteur ; Catarina Rosado, Auteur Année de publication : 2022 Article en page(s) : p. 333-342 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Cellules -- Cultures et milieux de culture Chimie analytique Chromatographie Dermo-cosmétologie Espèces réactives de l'oxygène Peau -- Soins et hygiène Propolis Tests de sécurité Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Propolis has been used since antiquity, but recent reports of its biological properties hint that it could be employed as a topical pharmaceutical and cosmetic ingredient. This work aims to probe the action of Portuguese propolis extracts on skin cells, providing mechanistic insights into its mode of action and preliminarily assessing its applicability as a skin repair ingredient. - Methods : The total phenolic content of propolis extracts was measured by the Folin Ciocalteu method. The cytotoxic effect of propolis extracts in human keratinocytes was determined and non-cytotoxic concentrations of the extracts were used to study the impact on collective cell migration, cell cycle and intracellular ROS levels. - Results : o significant impact was observed in collective cell migration, but one of the extracts mildly increased G2 phase while reducing the % of sub-G1 at a non-cytotoxic concentration. The two extracts with higher phenolic content strongly prevented intracellular cellular ROS accumulation upon exposure to TBHP. Collectively, these results indicate that the putative beneficial effects of propolis extracts in skin repair may not be attributable to induction of collective cell migration but could be partially ascribed to the protection from oxidative stress, which could act in synergy with its well-known antimicrobial activity. - Conclusion : These data support the applicability of this material in topical and cosmetic formulations and further in vivo assays should be conducted to fully characterize its efficacy and safety. Note de contenu : - MATERIALS AND METHODS : Chemicals - Preparation of propolis extracts and wax fraction - Total phenolic content and chromatographic analysis of the propolis extracts - 2D and 3D HaCaT cell culture - Cell cycle progression of HaCaT cells treated with the propolis extracts - In vitro wound healing assay - Impact of propolis extracts on intracellular reactive oxygen species - Statistical analysis - RESULTS : Phenolic profile of the propolis ethanolic extracts - Cell viability of HaCaT cells treated with the propolis extracts - Cell cycle progression of HaCaT cells treated with the propolis extracts - In vitro wound healing assay - Impact of propolis extracts on intracellular reactive oxygen species - Table 1 : Total phenolic content of propolis extracts DOI : https://doi.org/10.1111/ics.12781 En ligne : https://drive.google.com/file/d/1_f9EPPGbvAUvynjZ9XKivdTaZJuXxNl8/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=38135 [article]

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