A Harungana madagascariensis extract with retinol-like properties: Gene upregulations and protein expressions in human fibroblasts and skin explants / Richard Fitoussi in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 44, N° 2 (04/2022)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 2 (04/2022) . - p. 201-215 Titre : A Harungana madagascariensis extract with retinol-like properties: Gene upregulations and protein expressions in human fibroblasts and skin explants Type de document : document électronique Auteurs : Richard Fitoussi, Auteur ; Marie-Christine Branchet, Auteur ; Natacha Garnier, Auteur ; Gallic Beauchef, Auteur ; Alex Nkengne, Auteur ; Katell Vié, Auteur ; Sylvie Boisnic, Auteur Année de publication : 2022 Article en page(s) : p. 201-215 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Dermo-cosmétologie Explant de peau Expression génique Extraits de plantes:Extraits (pharmacie) Fibroblastes Peau -- Anatomie Peau -- Physiologie Vitamine A Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : Because they limit, even reverse, age-induced skin alterations, retinoids became a staple in cosmetology. However, their use can result in undesired secondary effects and there is a demand for natural sources of compounds with retinoid-like effects. A preliminary screening identified a Harungana madagascariensis plant extract (HME) as possibly inducing genes stimulated by retinol. We analysed its effect on gene and protein expression, comparing it to retinoids. - Methods : Gene expression was analysed by real-time qPCR on RNA from isolated fibroblasts subjected to retinol or the plant extract for 6, 48 or 96 h. Skin markers were quantified in fibroblasts cultured with retinol or extract containing medium, and UV-aged skin explants subjected to topical applications of creams containing retinol, retinaldehyde or HME. - Results : Real-time qPCR shows that the extract induced all RARs and RXRs, even RXRγ that was not induced by retinol. Eighty-eight per cent of the 25 early retinoid reaction genes induced by a concentration of retinol are induced by the extract. In fibroblasts, only the extract increased collagen III labelling, while collagen I and fibronectin labelling are increased by retinol and the extract, with higher levels for the extract. When topically applied to UV-aged skin explants, only the cream containing the HME led to increased labelling of CRABP1 in the epidermis. CRABP2 and Ki67 are induced by all three creams and no effect was detected on RXRs. In the dermisthe extract containing cream increased CRABP2, total collagen, procollagen I and collagen I while creams with retinol or retinaldehyde only affected some of these proteins. - Conclusions : The HME induces an overall retinol-like gene induction profile in isolated fibroblasts and retinoid-like stimulation of protein synthesis in both isolated fibroblasts and photoaged skin explants. Note de contenu : - MATERIALS AND METHODS : Skin explants - Preparation of fibroblast culture - Treatment of fibroblasts with retinol or HME - Preparation of RNA, reverse transcription and real-time qPCR Immunofluorescent quantification of collagens and fibronectin in fibroblasts - UV-induced ageing of skin explants and topical treatment with creams containing retinol, retinaldehyde or HME - Immunolabeling of skin fragments - ELISA quantification of procollagen I - Spectrocolourimetric quantification of elastin - Statistical analysis - RESULTS : Real-time qPCR comparative gene expression between isolated fibroblasts cultured in the presence of retinol or HME - Expression levels of collagen and fibronectin in isolated fibroblasts cultured in the presence of retinol or HME - Comparison of the effect of commercially available creams containing retinol or retinaldehyde with those induced by a cream containing the HME on the expression of proteins in UV-aged skin explants - Table 1 : Median of the maximum induction folds for the 27 genes tested by real-time qPCR in isolated fibroblasts subjected to retinol or HME - Table 2 : Pearson's correlation coefficients of the maximum induction fold for the 27 genes tested by real-time qPCR in isolated fibroblasts subjected to retinol or HME DOI : https://doi.org/10.1111/ics.12768 En ligne : https://drive.google.com/file/d/1osna4IvYNdLBuq-0tyJg-4r6AU5Y8VDF/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37723 [article]

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