Exploring compounds to be used as cosmetic agents that activate peroxisome proliferator-activated receptor alpha / Keisuke Tachibana in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 44, N° 2 (04/2022)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 2 (04/2022) . - p. 189-200 Titre : Exploring compounds to be used as cosmetic agents that activate peroxisome proliferator-activated receptor alpha Type de document : document électronique Auteurs : Keisuke Tachibana, Auteur ; Syohei Fukuda, Auteur ; Jun Fukushima, Auteur ; Kenji Ishimoto, Auteur ; Masahiro Sakata, Auteur ; Yasutomo Nishimori, Auteur ; Takefumi Doi, Auteur Année de publication : 2022 Article en page(s) : p. 189-200 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Analyse génétique Barrière cutanée Dermo-cosmétologie Extraits de plantes:Extraits (pharmacie) Kératinocytes Les kératinocytes sont des cellules constituant 90 % de la couche superficielle de la peau (épiderme) et des phanères (ongles, cheveux, poils, plumes, écailles). Ils synthétisent la kératine (kératinisation), une protéine fibreuse et insoluble dans l'eau, qui assure à la peau sa propriété d'imperméabilité et de protection extérieure. L'épiderme est divisé en 4 couches basées sur la morphologie des kératinocytes (de l'intérieur vers l'extérieur) : 1. stratum germinativum (couche basale à la jonction avec le derme) 2. stratum spinosum 3. stratum granulosum 4. stratum lucidum 5. stratum corneum Les kératinocytes passent progressivement de la couche basale vers les couches supérieures par différenciation cellulaire jusqu'au stratum corneum ou ils forment une couche de cellules mortes nommées squames, par apoptose. Cette couche constitue une barrière de protection et réduit la perte d'eau de l'organisme. Les kératinocytes sont en perpétuel renouvellement. Ils mettent environ 1 mois pour aller de la couche basale au stratum corneum mais ce processus peut être accéléré en cas d'hyperprolifération de kératinocyte (psoriasis). Peau -- Physiologie Peau -- Soins et hygiène Récepteurs cellulaires Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : The human epidermis is formed by the proliferation and differentiation of keratinocytes adjacent to the basement membrane. The outermost layer, the stratum corneum, is equipped with a barrier function that prevents water evaporation, and intercellular lipids play an important role in this barrier function. When the barrier is functioning normally, evaporation is prevented; however, when barrier function is impaired, moisture evaporates, resulting in dry and rough skin. Therefore, maintenance of normal barrier function is critical for maintaining normal skin function. Peroxisome proliferator-activated receptor α (PPARα) is mainly not only involved in lipid metabolism in the liver but is also expressed in the epidermis and is involved in inducing keratinocyte differentiation, promoting lipid production, maintaining barrier function and suppressing skin inflammation. Hence, compounds that activate PPARα are expected to control skin function. Therefore, we identified PPARα activators from among extracts of natural resources that have been approved for use in humans and analysed the effects of these extracts on skin function. - Methods : First, extracts of 474 natural resources were screened using a PPARα activator screening cell line independently constructed in our laboratory. Next, reporter assays were performed using the Gal4-chimera system to evaluate whether these extracts act as ligands for PPARα. We then analysed their effect on primary normal human epidermal keratinocyte cells by using real-time RT-PCR. Finally, we evaluated PPARα activation effect by the combination of these extracts. - Results : We identified 36 extracts having the effect of activating PPARα. In particular, #419, a Typha angustifolia spike extract, showed concentration-dependent transcriptional activation through PPARα-LBD and was considered to be likely to contain a compound that is a ligand of PPARα. #419 increased the expression of PPARα target genes and genes related to skin function in primary cultured human epidermal keratinocytes. Finally, the use of #419 in combination with nine extracts increased PPAR activity more than twice as much as #419 alone treatment. - Conclusions : These results showed that the reporter cell line could be useful for discovering extracts of natural resources and that the identified Typha angustifolia spike extract could be used in cosmetics that activate PPARα, which expected to improve skin function. Note de contenu : - MATERIALS AND METHODS : Reagents - Extract preparation of natural resources - Cell culture - Luciferase assays using a human PPARα reporter cell line - Gal4-chimera reporter gene assay - RNA extraction and quantitative real-time RT-PCR - Statistical analysis - RESULTS : Screening extracts of natural resources that activate PPARα - Analysis of the effects of screened extracts on nuclear receptors - Analysis of the effect of the extract #419 on skin function - Evaluation of PPARα activation effect by the combination of #419 and other extracts - Table 1 : Primers used for real-time PCR - Table 2 : Transcriptional activation of GAL4-nuclear receptors attributable to extracts #272 and #419 - Table 3 : List of extracts activated the PPARα activity DOI : https://doi.org/10.1111/ics.12767 En ligne : https://drive.google.com/file/d/1OZoy7J_w4OvSm_iYqz34POVe4bH_c4bd/view?usp=shari [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37722 [article]

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