Toxicity evaluation of collagen hydrolysates from chrome shavings and their potential use in the preparation of amino acid fertilizer for crop growth / Li Zhao in JOURNAL OF LEATHER SCIENCE AND ENGINEERING, Vol. 4 (Année 2022)  

[article]  inJOURNAL OF LEATHER SCIENCE AND ENGINEERING > Vol. 4 (Année 2022) . - 23 p. Titre : Toxicity evaluation of collagen hydrolysates from chrome shavings and their potential use in the preparation of amino acid fertilizer for crop growth Type de document : texte imprimé Auteurs : Li Zhao, Auteur ; Shengdong Mu, Auteur ; Weixiang Wang, Auteur ; Gu Haibin, Auteur Année de publication : 2022 Article en page(s) : 23 p. Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Acides aminés Les acides aminés (ou aminoacides) sont une classe de composés chimiques possédant deux groupes fonctionnels : à la fois un groupe carboxyle –COOH et un groupe amine –NH2. Parmi ceux-ci, les acides α-aminés se définissent par le fait que leur groupe amine est lié à l'atome de carbone adjacent au groupe acide carboxylique (le carbone α), ce qui leur confère la structure générique H2N–CHR–COOH, où R représente la chaîne latérale, qui identifie l'acide α-aminé. Les acides α-aminés jouent un rôle fondamental en biochimie comme constituants élémentaires des protéines : ils polymérisent en formant des liaisons peptidiques qui aboutissent à de longues chaînes macromoléculaires appelées peptides. Analyse toxicologique Chrome Collagène -- Analyse Engrais organiques Evaluation Hydrolysat de collagène Poisson zèbre Solubilité Tests de toxicité Index. décimale : 675.2 Préparation du cuir naturel. Tannage Résumé : Resource utilization of chrome shavings (CS) has attracted a lot of attention from scientists and technologists in leather industry. Especially, the collagen hydrolysates extracted from CS are expected to find potential application values in agricultural field. However, there is no biotoxicity analysis of collagen hydrolysates from CS. Herein, the collagen hydrolysates with different molecular weights were produced from CS by three hydrolysis dechroming methods including alkaline hydrolysis, enzymatic hydrolysis and alkaline-enzymatic synergistic hydrolysis, and the optimal hydrolysis process of CS was designed and conducted. To evaluate their toxicity, the three collagen hydrolysates were formulated into a nutrient solution for zebrafish development. The obtained results indicated that the hydrolysates with low concentrations (less than 0.6 mg/mL) were safe and could promote the development for zebrafish embryos. Furthermore, the three collagen hydrolysates were utilized as organic nitrogen sources and formulated into amino acid water-soluble fertilizers (AAWSF) including alkaline type fertilizer (OH), enzymatic type fertilizer (M) and alkaline-enzymatic type fertilizer (OH–M) for the early soilless seeding cultivation of wheat, soybean and rapeseed. It is worth mentioning that the chromium contents in the prepared AAWSF were less than 10 mg/kg, which is far less than the limit value in the standard (China, 50 mg/kg). The growth and development of seedlings (germination rate, plant height, fresh weight of leaves, soluble sugar content and chlorophyll content) were investigated. The corresponding results showed that the growth of seedlings watered with AAWSF was better compared with the other treatments, and the OH–M fertilizer had the best promoting effect on the seedlings growth and development, followed by the M and OH fertilizers. The safe toxicity assessment of the collagen hydrolysates will expand their application scope, and the use of collagen hydrolysates extracted from CS for seedlings growth also provides an effective and reasonable way to deal with the chromium-containing leather solid waste, which is an effective way to realize its resource utilization. Note de contenu : - EXPERIMENTAL : Materials - Preparation of collagen hydrolysate - Analysis of collagen hydrolysate - Zebrafish husbandry and embryos collection - Embryonic acute toxicity test - Preparation of AAWSF - Analysis of AAWSF - Crops seeding and growth experiments - Growth determination of seedlings - RESULTS AND DISCUSSION : Preparation of collagen hydrolysate - Analysis of collagen hydrolysates - Mortality, hatchability and medial lethal concentration of zebrafish embryos - Morphological development and deformity of zebrafish embryos - Body length and cardiomyogenesis of zebrafish embryos - General analysis of amino acid water-soluble fertilizer (AAWSF) - Effect of different AAWSF on the macroscopical growth of various seedlings - Effect of different AAWSF on the contents of Cr, N, P and K, chlorophyll and soluble sugar in seedlings - Table 1 : The pH value, protein and ash contents of ACH, ECH and AECH - Table 2 : The content of heavy metal in ACH, ECH and AECH - Table 3 : Amino acid composition of ACH, ECH and AECH DOI : https://doi.org/10.1186/s42825-021-00072-1 En ligne : https://link.springer.com/content/pdf/10.1186/s42825-021-00072-1.pdf Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37566 [article]

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