Heterologous expression of metalloproteinases from planococcus halotolerans SCU63T and eco-friendly enzymatic dehairing of goatskin / Zhang Shihao in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 105, N° 3 (05-06/2021)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 105, N° 3 (05-06/2021) . - p. 132-139 Titre : Heterologous expression of metalloproteinases from planococcus halotolerans SCU63T and eco-friendly enzymatic dehairing of goatskin Type de document : texte imprimé Auteurs : Zhang Shihao, Auteur ; Ruoshi Zhang, Auteur ; Xiaoguang Li, Auteur ; Zhe Xu, Auteur ; Yongqiang Tian, Auteur Année de publication : 2021 Article en page(s) : p. 132-139 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Caractérisation Cuirs et peaux de chèvres Enzymes microbiennes Epilage enzymatique Métalloprotéinases Microscopie électronique à balayage Peptidases pH Produits chimiques -- Purification Travail de rivière (cuir) Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : In this study, a metalloproteinase gene from Planococcus halotolerans SCU63 was cloned and expressed in Bacillus sp. SCK6. LB broth medium was used as the initial medium and for a series of fermentation optimisations. The most suitable medium components were as follows (g/l) : yeast extract powder (20), casein (13), tryptone (7), NaCl (10), urea (8). When the inoculation amount was 2%, the filling amount was 100mL, the culture time was 370C, and the pH was 7.0 for 40 hours the protease production reached the highest. The enzyme activity was completely inhibited by EDTA. Surfactants, such as Tween 20, Tween 80, and TritonX-100, could improve the enzyme activity, while SDS could inhibit the enzyme activity. Under protease action, goatskin can be completely depilated at 37oc and 28 hours. Compared with the conventional chemical dehairing, the enzymatic dehairing opened up the goatskin elastic fibre without any hair root in the hair follicle, and the skin was of high quality. Note de contenu : - MATERIALS AND METHODS : Materials - Construction of recombinant plasmid and expression of 1030 in B. subtilis SCK6 - Optimisation of fermentation conditions - Purification of proteases - Determination of enzyme activity - Characterisation of enzymatic properties - Dehairing function of protease 1030 - Analysis of dehairing - RESULTS : Construction of recombinant plasmid and its expression in SCK6 - Optimisation of fermentation conditions and RSM - Purification and identification of proteases 1030 - Effect of temperature on enzyme activity and stability - Effect of pH on enzyme activity and stability - Effect of NaCl concentration on enzyme activity - Effect of metal ions on enzyme activity - Effect of chemicals on enzyme activity - Effect of organic solvents on enzyme activity - Analysis of enzymatic dehairing - Analysis of SEM and staining - Table 1 : Factors and levels for RSM - Table 2 : ANOVA for quadratic model - Table 3 : Effect of metal ions on enzyme activity - Table 4 : Effect of chemicals on enzyme activity - Table 5 : Effect of solvents on enzyme activity En ligne : https://drive.google.com/file/d/15BWASUeWYefKBb5gBjgfaEyNcAG7bjbz/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35833 [article]

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