Deformable liposomes as enhancer of caffeine penetration through human skin in a Franz diffusion cell test / E. Abd J. Gomes in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE, Vol. 43, N° 1 (02/2021)  

[article]  inINTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 43, N° 1 (02/2021) . - p. 1-10 Titre : Deformable liposomes as enhancer of caffeine penetration through human skin in a Franz diffusion cell test Type de document : document électronique Auteurs : E. Abd J. Gomes, Auteur ; C. C. Sales, Auteur ; S. Yousef, Auteur ; F. Forouz, Auteur ; K. C. Telaprolu, Auteur ; M. S. Roberts, Auteur ; J. E. Grice, Auteur ; P. S. Lopes, Auteur ; V. R. Leite-Silva, Auteur ; N. Andréo‐Filho, Auteur Année de publication : 2021 Article en page(s) : p. 1-10 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Barrière cutanée Caféine Essais (technologie) Formulation (Génie chimique) Liposomes Pénétration (physique) Perméation Polypropylène glycol Index. décimale : 668.5 Parfums et cosmétiques Résumé : - Objective : The permeation of hydrophilic molecules through the skin is still a challenge due to the barrier posed by stratum corneum, the outermost layer of the skin. Liposomes have frequently been used as carriers for different types of drugs and may also function as permeation enhancers. Propylene glycol has also been used as an edge activator in liposomes to increase the permeation. The aim of this work was to prepare liposomes containing an edge activator and loaded with caffeine to evaluate the potential of caffeine reaching the deeper layers in the skin. - Methods : The formulations were prepared by a top-down process using high-pressure homogenization at 200 00 psi for 10 min. They were characterized by size, polydispersity index (PI), zeta potential (ZP), pH, caffeine content and encapsulation efficiency (EE%) on preparation (time zero) and after 30 days. Cytotoxicity of blank and loaded liposomes was assessed by MTT proliferation assay with a normal keratinocyte cell line (HaCaT). In vitro permeation tests were performed with human skin in Franz cells over 24 h, and caffeine concentration was determined in the skin surface, stratum corneum, dermo-epidermal fraction and receptor medium by HPLC. - Results : The caffeine liposomes with (DL-Caf) or without propylene glycol (CL-Caf) showed, respectively, mean size 94.5 and 95.4 nm, PI 0.48 and 0.42, ZP + 1.3 and + 18.1 mV and caffeine content of 78.57 and 80.13%. IC50 values of caffeine in DL-Caf (3.59 v/v %) and CL-Caf (3.65 v/v %) were not significantly different from conventional blank liposome (3.27 v/v %). The DL-Caf formulation presented the best capability to enhance the caffeine permeation through the skin, resulting 1.94-folds higher than caffeine solution. Furthermore, the caffeine flux from DL-Caf was 1.56- and 3.05-folds higher than caffeine solution and CL-Caf, respectively. On the other hand, CL-Caf showed the lowest caffeine penetration revealing the importance of edge activator to aid hydrophilic drug penetration to all skin layers. - Conclusion : The DL-Caf formulation tested was able to improve the permeation of caffeine through the stratum corneum and dermo-epidermal layers, suggesting that this delivery system may be effective for deep skin delivery of hydrophilic drugs. Note de contenu : - MATERIALS AND METHODS : Materials - Preparation of liposomes - Characterization of liposome formulations - Caffeine quantification and encapsulation efficiency (EE%) - Cell culture studies - Treatment solutions - Cytotoxicity assay : MTT test - Caffeine skin permeation studies - Sample analysis - Data analysis and statistics - RESULTS AND DISCUSSION : Characterization of liposomes - Encapsulation Efficiency (EE%) - Cytotoxicity tests - Permeation of Caffeine from Liposomes and Controls - Table 1 : Liposome formulations and controls composition - Table 2 : Characterization of liposomes formulations data. Measurements were done at zero and thirtieth days. All data are results of three analyses (n = 3) - Table 3 : Content of caffeine in liposomes formulation and encapsulation efficiency (EE%) - Table 4 : IC50 values of conventional liposome and caffeine encapsulated on HaCaT cell line - Table 5 : Permeation parameters following 24-h application of caffeine from liposome formulations and controls to full-thickness human skin (mean SD, n = 3) DOI : https://doi.org/10.1111/ics.12659 En ligne : https://drive.google.com/file/d/1xwcDoaHKMJNtr-XBVeV4cz__-o2pSCsZ/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=35438 [article]

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