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Culture conditions optimisation of bacillus subtilis for producing bioflocculant / Changqing Zhao in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 104, N° 3 (05-06/2020)
[article]
Titre : Culture conditions optimisation of bacillus subtilis for producing bioflocculant Type de document : texte imprimé Auteurs : Changqing Zhao, Auteur ; Zhang Yizhuo, Auteur ; Yang Qinhuan, Auteur ; Yang Yang, Auteur Année de publication : 2020 Article en page(s) : p. 151-155 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bacillus subtilis
Bactéries -- Culture et milieux de culture
Biofloculant
Eaux usées -- Décontamination
Eaux usées -- EpurationIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : A bioflocculant seen as an environmentally friendly flocculant with potential for development, with the capacity to improve its activity and yield has become a hot topic of research among scholars in various countries. This study found that a bioflocculant produced by Bacillus subtils is able to treat leather wastewater, but the flocculation activity of the bioflocculant needs to be improved. In this paper, the culture conditions of Bacillus subtilis were optimised by single-factor and orthogonal tests. The results showed that the bioflocculant produced by Bacillus subtilis through optimising the culture conditions had good flocculation efficiency. The optimal culture temperature after optimisation was 36°C, the inoculum dose was 5% (v/v), at pH7.5, the culture time was 48 hours, the flocculation efficiency increased from 46.12% to 73.71%, an increase of 59.82%, and the production of flocculant increased from 0.45g/L to 0.61g/L, an increase of 35.56%. Through optimising the culture conditiops of Bacillus subtilis the flocculation efficiency of the bioflocculant, was improved and both the cost of flocculation and secondary pollution were reduced. Note de contenu : - EXPERIMENTAL PROCEDURE : Materials - Activation of Bacillus subtilis species - Initial culture of Bacillus subtilis - Collection of bioflocculant
- SINGLE FACTOR OPTIMISATION OF CULTURE CONDITIONS : Determination of culture temperature - Determination of inoculum dose - Determination of culture pH - Determination of culture time
- ORTHOGONAL OPTIMISATION OF CULTURE CONDITIONS
- RESULTS AND DISCUSSION : Effect of culture temperature - Determination of optimum inoculum dose - Determination of optimal pH - Determination of optimum culture time - Orthogonal test of culture conditions - Comparison of effects before and after optimisation of culture conditionsEn ligne : https://drive.google.com/file/d/1ypFkJYJntpsnfPxiGliF-VWD9zQ9BMcj/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34245
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 104, N° 3 (05-06/2020) . - p. 151-155[article]Réservation
Réserver ce documentFlocculation mechanism of modified bioflocculant for leather wastewater treatment / Zhang Yizhuo in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 104, N° 4 (07-08/2020)
[article]
Titre : Flocculation mechanism of modified bioflocculant for leather wastewater treatment Type de document : texte imprimé Auteurs : Zhang Yizhuo, Auteur ; Yang Qinhuan, Auteur ; Changqing Zhao, Auteur Année de publication : 2020 Article en page(s) : p. 184-190 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Biomatériaux
Cuirs et peaux -- Industrie -- Aspect de l'environnement
Eaux usées -- Epuration
Eaux usées -- Recyclage
FloculationIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : Leather processing produces a large amount of wastewater with complex components. In particular, wastewater denitrification is a worldwide environmental problem. In this experiment, according to the characteristics of high concentration of suspended solid and heavy pollution load in leather wastewater, Bacillus cereus was used as the microbiota producing flocculant and its metabolites were collected as bioflocculant. Ampholytic starch was a novel selection for chemical modification, and the modified bioflocculant was used to treat leather wastewater. After determination, the removal rate of total nitrogen reached 100%, and the flocculation efficiency reached 98.17%. Exploring the flocculation mechanism of the modified bioflocculant for treating leather wastewater, shows that the modified bioflocculant and the pollutants in the wastewater were bonded by ionic bonds, and the particle size of supernatant in leather wastewater system gradually decreased from 2026nm to 147nm when the added modified bioflocculant charge was 0.08g, which indicated the existence of adsorption and bridging. In addition, the change of peak on the infrared spectrogram of the flocculation sediment indicated the chemical interaction of the functional groups such as -OH, -COOH in the flocculation process. When the pH of flocculation system rose from 4.0 to 8.0, the potential rose from -36mV to 1 mV, which proved that a strong charge neutralisation occurred. Through observing the appearance of particles in leather wastewater system, it was found that multi-network overlapping structure flocs were formed after the modified bioflocculant interacted with wastewater, and the flocs could capture the remaining pollutants in wastewater, which proved that sweep-floc coagulation occurred. The results showed that the modified bioflocculant combined with a variety of flocculation ways in the treatment of leather wastewater which enhanced the flocculation ability. This study clarified the flocculation mechanism of modified bioflocculant and further, provided a reference for the development of new more efficient bioflocculant which is environment friendly. Note de contenu : - EXPERIMENTAL PROCEDURE : Materials - Collection of bioflocculant - Preparation of leather wastewater - Determination of the flocculation efficiency - Determination of the total nitrogen
- EXPLORATION OF FLOCCULATION MECHANISM : The exploration of adsorption and bridging - The exploration of chemical interaction - The exploration of charge neutralisation - The exploration of sweep-floc coagulation
- RESULTS AND DISCUSSION : Performance determination of modified bioflocculant treatment of leather wastewater
- THE FLOCCULATION MECHANISM IN THE TREATMENT OF LEATHER WASTEWATER : The exploration of the adsorption and bridging - The exploration of chemical interaction - The exploration of charge neutralisation - The exploration of sweep-floc coagulation - Application and analysis of the potential of the modified bioflocculantEn ligne : https://drive.google.com/file/d/1Fj4XBve2Zagf1G1jPqWG1-13qg5OpfWA/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=34331
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 104, N° 4 (07-08/2020) . - p. 184-190[article]Réservation
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