| Titre : |
Preparation and characterization of decellularized ECM hydrogel from adipose tissues of hide/skin : Unlocking untapped resource potential |
| Type de document : |
texte imprimé |
| Auteurs : |
Gayathri Devi Chakrapani, Auteur ; Fathima Shifana, Auteur ; Gladstone Christopher Jayakumar, Auteur ; Balaraman Madhan, Auteur |
| Année de publication : |
2024 |
| Article en page(s) : |
p. 303-313 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Biomatériaux
Caractérisation
Cuirs et peaux -- Déchets -- Recyclage
Glycosaminoglycanes
hydrocolloïdes
Ingénierie tissulaire
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Raw fleshing wastes is the major solid waste generated during leather manufacturing. Utilization of the fleshing waste for the development of biomaterial holds a promising and sustainable solution for leather manufacturing. Decellularized hydrogels possess the ability to differentiate into specific tissue types of the host microenvironment and supports the cell adhesion, migration, proliferation, and differentiation of cells. Hydrogel is a biocompatible biomaterial that has the potential to mimic extracellular matrix (ECM). ECM-based biomaterials derived from animal tissues are ideal for applications in tissue engineering. In the present research work, dECM-derived hydrogels from the decellularized raw fleshing waste was prepared and characterized for its possible applications in tissue engineering. The fleshing waste was decellularized using varying concentrations of SDS and Triton-X 100 detergents. The tissue sample treated with 0.1% SDS and 1% Triton-X 100 for decellularization exhibited higher collagen and glycosaminoglycans (GAG) retention. The study also provides a possible way for the transformation of tannery fleshing waste into high value products. |
| Note de contenu : |
- EXPERIMENTAL : Materials - Preparation of decellularized fleshing tissue - Preparation of hydrogel from decellularized fleshing - Characterisation of decellularized - DNA content quantification - Collagen estimation assay - Glycosaminoglycans (GAGs) assay - Histological analysis - Fibrillation assay - Scanning electron microscopy (SEM) - Gelation kinetics - Methylene blue active substances (MBAS) assay - Cell viability and cytotoxicity studies
- RESULTS : DNA content quantification - Collagen estimation assay - Glycosaminoglycans (GAGs) assay - Histological analysis - Fibrillation assay and gelation kinetics - Microscopic analysis - Methylene blue active substances (MBAs) assay - Cell viability and cytotoxicity studies
- Table 1 : Chemical decellularization treatment protocols
- Table 2 : The concentration of GAGs (µg/mg) present in the sample
- Table 3 : Turbidity analysis results of decellularized ECM-based hydrogels |
| DOI : |
https://doi.org/10.34314/ay8wjt13 |
| En ligne : |
https://drive.google.com/file/d/1g4xEpzMNkBrQuebDIHGAuUqsOeL0--h2/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=41284 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIX, N° 7 (07/2024) . - p. 303-313
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Preparation and characterization of decellularized ECM hydrogel from adipose tissues of hide/skin : Unlocking untapped resource potential in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIX, N° 7 (07/2024)
