| Titre : |
Key mechanism of enzymatic dehairing technology for leather-making : permeation behaviors of protease into animal hide and the mechanism of charge regulation |
| Type de document : |
texte imprimé |
| Auteurs : |
Mengchu Gao, Auteur ; Jinzhi Song, Auteur ; Xu Zhang, Auteur ; Chunxiao Zhang, Auteur ; Biyu Peng, Auteur ; Sadaqat Ali Chattha, Auteur |
| Année de publication : |
2023 |
| Article en page(s) : |
18 p. |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Caractérisation
Epilage enzymatique
Follicule pileux
Peptidases
Perméation
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Enzymes have been widely used as alternatives to conventional chemicals in cleaner leather processes due to their advantages of meeting increasing environmental demands. Especially, enzymatic unhairing based on protease has been applied to leather-making for a long time, however, it still has the key problem of slow permeation in the animal hide, resulting in slow unhairing rate, poor hair removal effect, excessive proteolysis of hide collagen, and decreased leather quality. Aiming at the key problem of bio-unhairing technology, fluorescent labeling technique and confocal laser scanning microscopy were used to investigate the protease permeation behaviors into the animal hide based on well-prepared labeled proteases, as well as the quantitative analysis of the protease amount in different hide layers. The results show that the protease mainly permeates the bovine hide through transfollicular routes from the hair side, and although the intercellular-interfibrillar pathway also exists, it does not play an important role. Additionally, the protease permeation behaviors into the hide are greatly impacted by the charge states of the hide and protease proteins, depending on the isoelectric points (pI) of the proteins and solution pH values. When the solution pH is not between the pI values of the two proteins, the similarly charged protease can quickly and deeply penetrate the hide because of the electrostatic repulsion. The established mechanism provides a theoretical basis for developing an efficient enzymatic unhairing technology for leather-making, and this can also be applied to other processes involving the enzyme permeation into the hide or leather. |
| Note de contenu : |
- MATERIALS AND METHODS : Materials - Preparation and characterization of fluorescently labeled proteases - Permeation experiments - Confocal laser scanning microscopy analysis - Quantitative analysis of permeation process and dehairing experiment
- RESULTS AND DISCUSSION : Purification and stability of FITC-labeled proteases - UV–Vis and fluorescence spectroscopy characterizations of FITC-labeled proteases - Impact of fluorescent labeling on protease properties - Permeation behaviors of FITC-labeled proteases in the hide - Quantitative analyses of protease permeation processes and enzymatic dehairing effectiveness - Mechanism of charge regulation of protease permeation behaviors into the hide
- Table 1 : Proteolytic activities of FITC-labeled and unlabeled proteases (pH 8.0, 30℃) |
| DOI : |
https://doi.org/10.1186/s42825-023-00117-7 |
| En ligne : |
https://link.springer.com/content/pdf/10.1186/s42825-023-00117-7.pdf |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=40063 |
in COLLAGEN AND LEATHER > Vol. 5 (2023) . - 18 p.
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Key mechanism of enzymatic dehairing technology for leather-making : permeation behaviors of protease into animal hide and the mechanism of charge regulation in COLLAGEN AND LEATHER, Vol. 5 (2023)
