| Titre : |
The hydrolytic activities of two fungal species isolated from historical quranic parchment manuscript |
| Type de document : |
texte imprimé |
| Auteurs : |
Saada N. S., Auteur ; Gomaa Abdel-Maksoud, Auteur ; Ahmed M. Youssef, Auteur ; Mohamed S. Abdel-Aziz, Auteur |
| Année de publication : |
2018 |
| Article en page(s) : |
p. 141-148 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Antiquités (objets anciens) -- Analyse
Champignons microscopiques
Hydrolyse
Parchemin
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Two fungal strains were isolated from a historical parchment manuscript found in Al-Azhar library, Egypt and were identified using the molecular technique 18S rRNA. New parchment samples (see 2.1) were placed on the surface of agar plates and inoculated with the two identified fungi and incubated at 30°C for 10, 20 and 30 days. The parchment samples were checked for degradation by measuring its mechanical properties (elongation and tensile strength), and ATR-FTIR analysis to identify structural changes induced in the collagen triple helix. SEM analysis was also carried out to investigate the change of surface morphology of bio-deteriorated collagen fibres. The colour changes in the fungal-treated parchment samples were also examined in order to determine the proteolytic activity caused by the tested fungal strains and their impact on the parchment properties. The results obtained revealed that Aspergillus fumigatus and Talaromyces spectabilis denature collagen as a result of their proteolytic activity, leading to loss of mechanical properties and the fibre network, so the parchment becomes brittle and discoloured. |
| Note de contenu : |
- MATERIALS AND METHODS : New parchment samples - Sampling and fungal isolation
- Identification of the fungal strains (N1 and N2) - Identification of fungal cultures - Fungal degradation of the parchment samples - Mechanical properties - Fourier transform infrared spectroscopy (FTIR) - SEM examination - Colour change
- RESULTS AND DISCUSSION : Molecular identification of the fungal isolate N1 and N2 - mechanical properties - Fourier Transform Infrared Spectroscopy (FTIR) - Investigation of the surface morphology by SEM - Change of colour |
| En ligne : |
https://drive.google.com/file/d/1MAObPBVQX1iS6sYrheyyQwNsmY50T6Kx/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=30795 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 102, N° 3 (05-06/2018) . - p. 141-148
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