| Titre : |
Hide powder azure and azocoll as substrates for assay of the proteolytic activity of bate |
| Type de document : |
texte imprimé |
| Auteurs : |
Samuel M. Mozersky, Auteur ; David G. Bailey, Auteur |
| Année de publication : |
1992 |
| Article en page(s) : |
p. 287-295 |
| Note générale : |
Bibliogr. |
| Langues : |
Américain (ame) |
| Catégories : |
Collagène
Confitage enzymatique
Enzymes pancréatiques
Poudre de peaux
ProtéolyseEn biochimie, la protéolyse est la segmentation des protéines en ses fragments de base (acides aminés) via l'hydrolyse catalysée par des enzymes dits "protéolytiques" (protéases ou hydrolases). (Wikipedia)
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Hide powder azure (HPA) and azocoll (Az) were evaluated for their suitability as collagen-rich substitutes for casein in assaying for the proteolytic activity of bate. Optimum conditions were established and a recommended procedure is presented. Under these conditions, the proteolytic activity of bate can be evaluated using an incubation period of one minute. Commercially available HPA and Az of small particle size were both found to be suitable substrates, provided the prescribed conditions are carefully observed. Evaluation of a commercially available preparation of coarse Az indicated that it is less suitable as a substrate for the assay. |
| Note de contenu : |
- Incubation buffer
- Agitation
- Termination of enzyme action
- Washing the substrate
- Time course of proteolysis
- Influence of the amount of bate
- Pancreatic bate
- Precision
- Recommended procedure
- Table 1 : Precision of measurement of reaction velocity |
| En ligne : |
https://drive.google.com/file/d/1kFYIu78JLplzs6CVejKWxTa2W9jwWtAw/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39841 |
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. LXXXVII, N° 8 (08/1992) . - p. 287-295
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Hide powder azure and azocoll as substrates for assay of the proteolytic activity of bate in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. LXXXVII, N° 8 (08/1992)
