| Titre : |
The characterisation and depilation effect of extracellular proteases of streptomyces sp. TYQ1024 |
| Type de document : |
texte imprimé |
| Auteurs : |
Chufan Cai, Auteur ; Xiaoguang Li, Auteur ; Hongbin Wang, Auteur ; Yiwen Chu, Auteur ; Yongqiang Tian, Auteur |
| Année de publication : |
2022 |
| Article en page(s) : |
p. 120-125 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Epilage enzymatique
Essais (technologie)
Peau -- Histologie
Peptidases
|
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
A strain that was highly active at producing protease was isolated from soit, identified as a species of Streptomyces and designated TYQ1024. A genomic analysis showed that it contained a variety of protease genes. The crude enzyme obtained from the fermentation broth had a high level of proteolytic activity and was activated by 5mM of metal ions, including Ca2+, Sr2+, Ba2+, and highly inactivated by 5mM of Cu2+, Zn2+ and Fe3+. The protease secreted by this strain could efficiently dehair rabbit skin after 24 hours of incubation at 40°C without damaging the collagen dermal fibres, which demonstrates its potential application for processing leather. |
| Note de contenu : |
- MATERIALS AND METHODS : Culture and maintenance - 16S rDNA and phylogenetic analysis - Protease gene analysis - Protease fermentation and activity test - Effects of temperature and ions on protease activity - Dehairing test and histological studies
- RESULTS AND DISCUSSION : Basic information and protease - Protease activity - Dehairing performance of TYQ1024 protease |
| En ligne : |
https://drive.google.com/file/d/15TAmmhR1Jb4NtcqnsrJDrTTcPia2xnIo/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37756 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 106, N° 3 (05-06/2022) . - p. 120-125
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The characterisation and depilation effect of extracellular proteases of streptomyces sp. TYQ1024 in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 106, N° 3 (05-06/2022)
