[article]
| Titre : |
A novel epigenetic-regulating active for repairing weakened and damaged skin |
| Type de document : |
texte imprimé |
| Auteurs : |
Snow Hsieh, Auteur ; Kuan-Yi Yeh, Auteur |
| Année de publication : |
2019 |
| Article en page(s) : |
p. 16-22 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Anti-inflammatoires
Biomolécules actives
Cicatrisation
Cosmétiques
Dermo-cosmétologie
Peau -- Soins et hygiène
Régénération (biologie)
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
Skin lesions not only have negative social and psychological ramifications on both men and women but may also cause irritation, discomfort, burn and even pain. Uneven skin tone, pigmentation, fine lines and wrinkles as well as scaling and flaky skin are all considered signs of imperfect skin, but one of the more permanent types of skin damage is skin lesions that may potentially lead to severe scarring. These scars are typically punctured deep and can only be removed through a series or combination of clinical procedures.
Even to a less severe extent, skin lesions may leave skin with temporary pigmentation, marks and blemishes. If appropriate treatment is not applied on the damaged skin site, skin lesions, especially those resulting from inflamed acnes, may leave large surface of skin rough and bumpy. Besides naturally occuring skin damage, there are numerous clinical procedures, such as laser treatment, skin needling or microdermabrasion, aiming at weakening or destroying the stratum corneum or slightly deeper skin layer to trigger the innate rejuvenating and healing mechanism of skin. This post-procedure skin undergoing dermabrasion or laser treatment is slightly inflamed on the surface and typically sensitive, as the skin barrier was dwindled and enfeebled. Regardless of the perpetual tendency for human to pursue beauty defined by flawless skin or the contemporary concept of wellness and healthy living, the marks and scars as well as the weakened, damaged or disfigured surface left on the skin instigate negative psychological impacts and sequelae in the forms of embarrassment, impaired self-image, low self-esteem or even anxiety. Therefore, soothing and repairing weakened skin as well as damaged skin has been an important area for cosmetic development. |
| Note de contenu : |
- MECHANISM OF SKIN REPAIR & SCARRING
- EPIGENIC REGULATION & PROPERTIES OF EPI-ON
- IN VITRO STUDIES:SKIN REPAIR AND PROTECTIVE EFFECT VIA EPIGENIC PATHWY : Epi-On upregulates expression of growth factor and accelerates tissue regeneration throught epigenetic regulation - Epi-On promotes profiferation and migration of both Keratinocytes and Fibroblasts
- IN VITRO STUDIES:ANTI-INFLAMMATORY & 5α-REDUCTASE INHIBITION : Epi-On attenuates inflammatory reactions - Epi-On reduces 5α-reductase activity
- EX VIVO STUDY:SKIN REPAIR AND BARRIER STRENGTHENING ACTIVITIES ON HUMAN SKIN EXPLANTS
- Fig. 1 : The cutaneous wound healding is a complex and dynamic process, comprising three major overlapping phases: (A) inflammation phase, where blood clot starts forming and leukocytes triggers inflammatoty responses; (B) cell proliferation, phase, where new fibroblast grows contributing to tissue formation; and (C) matrix remodeling, where the contraction and closure of wound site can be observed as neoepidermis and/or scar tissue forms.
- Fig. 2 : Epi-On attenuated HDAC activity of HaCaT (crude nuclear extract) in dose-depend manner. An effective HDAC inhibitor Trichostatin A (TsA) was used as positive control of this HDAC activity.
- Fig. 3 : Epi-On up-regulated the acetylation of (A) HAK16 in HaCaT cell line and (B) Hs68 cell line. Trichostain A (TsA) was used as the positive control of the western blot assay.
- Fig. 4 : (A) Representative western blot bands of H4K16ac and β-actin of H2O2-induced NHDF after Epi-On treatment. (B) H2O2 was used to remove the acetyl group from H4K16, and Epi-On inhibit H2O2-triggered H4K16ac deacetylation in NHDF in dose-dependent manner.
- Fig. 5 : (A) Microscopic images of wounded monolayer keratinocytes (HaCaT) after Epi-On treatment (24hr) and (B) 5,05% Epi-On (Al) significantly promoted proliferation and migration on keratinocytes cell line (+153,9%)
- Fig. 6 : (A) Microscopic images of wounded monolayer fibroblasts (Hs68) after Epi-On treatment (18hr) and (B) 0,05% Epi-On (Al) significantly promoted proliferation and migration on fibroblasts cell line (+84,4%)
- Fig. 7 Epi-On significantly reduced two inflammatory cytokines IL-6 and IL-8 proteins on LPS-treated HaCaT cell line to 72,7% and 73,5%, respectively
- Fig. 8 : 0,6%, 0,4% and 0,2% Epi-On decreased 5alpha-reductase activity to 66,6%, 81,5% and 91,7% respectively. 5alpha-reductase is an enzyme responsible for converting testosterone to dihydrotestosterone (DHT), which can potentially inhibit re-epitheliazation.
- Fig. 9 : The application of 4% Epi-On progressively and significantly promoted the thickening of stratum corneum as protective barrier on Day 3 and Day 10, as shown by the top-layered red stains.
- Table 1 : Summary of growth factor gene expression result - these genes are involved in the proliferation and differentiation of keratinocytes and fibroblast. |
| En ligne : |
https://drive.google.com/file/d/1p3Tm7SHQsTUhuJnIg3sBm8dtyu63ocsF/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=32000 |
in SOFW JOURNAL > Vol. 145, N° 3 (03/2019) . - p. 16-22
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A novel epigenetic-regulating active for repairing weakened and damaged skin in SOFW JOURNAL, Vol. 145, N° 3 (03/2019)
