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Covalent immobilization of organophosphorus hydrolase onto insoluble bovine collagen fibers / Yanhong Li in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CIX, N° 6 (06/2014)
[article]
Titre : Covalent immobilization of organophosphorus hydrolase onto insoluble bovine collagen fibers Type de document : texte imprimé Auteurs : Yanhong Li, Auteur ; Xinju Jia, Auteur ; Biyu Peng, Auteur Année de publication : 2014 Article en page(s) : p. 197-206 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Caractérisation
Cinétique chimique
Glutaraldéhyde
Hydrolase
pH
Poudre de peaux
TempératureIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : An organophosphorous hydrolase (OPH) was prepared and partially purified from Flavobacterium sp. The crude enzyme, with an activity of 1030U/g, was immobilized onto insoluble bovine collagen fibers (hide powder), instead of collagen membrane, through glutaraldehyde coupling. Optimal conditions of enzyme immobilization and properties of the immobilized enzyme preparation were investigated. Compared to glutaraldehyde tanned and chrome tanned hide powders, non-tanned hide powder showed higher immobilized enzyme activity. The optimal enzyme immobilization conditions are as follows: 10mg of enzyme was immobilized onto 500mg of hide powder in a 50mM phosphate buffer of pH 7.5 at 20°C, and 20% glutaraldehyde offer (based on the hide powder weight) was used to couple the enzyme and collagen fibers. The enzyme activity yield was about 35% and the hide powder immobilized OPH has an enzyme activity of about 7U/g. The immobilized enzyme showed the same temperature and pH profiles as the free enzyme, and it performed at much higher pH and with better thermal stability. The Km value of the immobilized enzyme was a somewhat higher (0.388mM) than that of the free enzyme (0.215mM). The reusability test showed that about 85% activity was retained after 10 use cycles. After storing for eight months at 20°C, the residual activity of the immobilized enzyme preparation was 98%. Note de contenu : - MATERIALS AND METHODS : Materials - Methods (Preparation and partial purification of organophosphorus hydrolase (OPH) from flavobacterium sp. - Preparation of tanned hide powder - OPH activity assay - OPH activity stability against glutaraldehyde - Immobilization of OPH on hide powders - Activity assay and characterization of immobilized OPH)
- RESULTS AND DISCUSSION : The influence of glutaraldehyde on OPH activity - Factors affecting immobilization of OPH on hide powder (OPH immobilized on different hide powders - Effect of pH - Effect of glutaraldehyde dosage - Effect and temperature - Effect of added enzyme amount) - Characterization of immobilized OPH (pH and temperature-activity profiles - pH and thermal stability of immobilized enzyme - Kinetics parameters - Operational and storage stability)En ligne : https://drive.google.com/file/d/1-aAAxLzbsyLkcO9tzzpc_gUMX5hFAqhP/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=21518
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CIX, N° 6 (06/2014) . - p. 197-206[article]Réservation
Réserver ce documentSynthesis of multi-site polyether amine triazine derivative for sustainable leather manufacturing / Xinju Jia in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXVII, N° 4 (04/2022)
[article]
Titre : Synthesis of multi-site polyether amine triazine derivative for sustainable leather manufacturing Type de document : texte imprimé Auteurs : Xinju Jia, Auteur ; Ran Tan, Auteur ; Biyu Peng, Auteur Année de publication : 2022 Article en page(s) : p. 141-152 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Analyse thermique
Blancheur
Cuirs et peaux -- Propriétés mécaniques
Cuirs et peaux -- Propriétés physiques
Environnement -- Etudes d'impact
Epaisseur -- Mesure
Morphologie (matériaux)
Polyéther amine triazine
Polymères -- Synthèse
Potentiel zeta
Tannage synthétique
Température de retraitIndex. décimale : 675 Technologie du cuir et de la fourrure Résumé : In this work, a multi-site polyether amine triazine derivative (ET) was prepared via a one-step process and its structure was confirmed using FT-IR and 1H NMR analyses. Then, the ET was applied to the tanning process and the ET tanning approach optimized and evaluated. The result indicated that after tanning with 8% ET at pH 6 for 6 h, the shrinkage temperature of leather was 83.9°C and the thickness change rate was 97.5%. The XRD, DSC, TG, SEM and Zeta potential analyses demonstrated that ET could couple with the amino groups of the collagen side chains and breaking partial hydrogen bonds between fibers, brought significant improvement to the softness of leather without obvious damage to the high-level structure of collagen. Residual Cl- concentration in the wastewater
decreased 42.1% in the tanning process compared to the conventional chrome tanning method. The emissions of COD and TDS in the tanning process reduced by 40.5% and 27.2%, respectively. It is conceivable that the ET tanning approach provides an improved approach to promote leather industry to develop sustainably.Note de contenu : - EXPERIMENTAL : Materials and equipment - Preparation of polyether amine triazine derivatives (ET) - Fourier Transform Infrared Spectroscopy (FT-IR) - Nuclear magnetic resonance spectroscopy (1H NMR) - Leather tanning processing - Determination of the shrinkage temperature - Determination of the exhaustion rate of tanning agent - Determination of the percent thickness increase - Thermal analysis - Zeta potential analyses - Morphological analyses - Whiteness analysis - X-ray diffraction (XRD) measurement - Determination of physical and mechanical properties - Environmental impact assessment
- RESULTS AND DISCUSSIONS : Characterization of ET - Optimization of the ET tanning conditions - Optimization of the ET tanning temperature - Optimization of the ET dosage - Zeta potential analysis - XRD analysis - Physical performance leather - Whiteness analysis - Morphology analysis - Environmenta impact assessments
- Table 1 : ET tanning process
- Table 2 : CTDT tanning process
- Table 3 : Tanning effect of different ET dosage
- Table 4 : Weight loss in different thermogravimetric stage
- Table 5 : Physical performances of leather tanned with different method
- Table 6 : Whiteness of wet-white tanned by different methodsDOI : https://doi.org/10.34314/jalca.v117i4.4897 En ligne : https://drive.google.com/file/d/1aScYsv5HLo94PmHjL2bri8iHG1jRufx0/view?usp=drive [...] Format de la ressource électronique : Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37561
in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXVII, N° 4 (04/2022) . - p. 141-152[article]Réservation
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