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Leather species identification via mitochondrial DNA polymerase chain reaction / Xiang Zhang in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIV, N° 7 (07/2019)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIV, N° 7 (07/2019) . - p. 265-270 Titre : Leather species identification via mitochondrial DNA polymerase chain reaction Type de document : texte imprimé Auteurs : Xiang Zhang, Auteur ; Steve Lange, Auteur Année de publication : 2019 Article en page(s) : p. 265-270 Note générale : Bibliogr. Langues : Américain (ame) Catégories : ADN mitochondrial Cuirs et peaux -- Analyse Cuirs et peaux -- Identification Génétique animale Réaction en chaîne par polymérase Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Currently the identification of leather species is mainly via a skilled operator using optical microscopy and an extensive visual reference library. However, due to the limitations of optical microscopy and in cases where the leather surface is heavily corrected or finished, it is not always possible to accurately determine the leather species. Therefore, it is crucial to develop a reliable and easy to perform approach to overcome these limitations. In this paper, we used the unique DNA sequence present in every species to identify leather. The conversion process of making leather destroys much of the DNA present but the high copy number of mitochondrial DNA (mtDNA) in relation to nuclear DNA makes some mtDNA likely to survive and be detected via polymerase chain reaction (PCR). However, leather DNA is highly degraded and inhibitors present in the extracted DNA make successful PCR challenging. There are only a few related works reported in the literature attempting to use DNA for leather species identification and they provide limited technical details. In this study, species-specific primers for PCR were designed, conditions for DNA extraction from leather followed by PCR were optimized, and a detailed protocol was also provided. As a proof of concept, our approach demonstrated that the species of leather can be reliably detected via PCR targeting mtDNA. Note de contenu : - EXPERIMENTAL : Reference DNA - Leather DNA extraction - mtDNA sequence analysis and primer design - Leather species identification via mtDNA PCR - PCR product analysis via agarose gel electrophoresis - RESULTS AND DISCUSSION : Reference genomic DNA preparation - Leather DNA extraction - Species-specific primer design - Optimization of PCR condition En ligne : https://drive.google.com/file/d/1LSX8z8UCK5XOkjgexJmC7lyWH6zZR4KQ/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=32785 [article]

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Limiting microbial activity as an alternative approach of bovine hide preservation, Part I : Efficacy of developed formulations / Majher I. Sarker in JOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA), Vol. CXIV, N° 7 (07/2019)  

[article]  inJOURNAL OF THE AMERICAN LEATHER CHEMISTS ASSOCIATION (JALCA) > Vol. CXIV, N° 7 (07/2019) . - p. 271-278 Titre : Limiting microbial activity as an alternative approach of bovine hide preservation, Part I : Efficacy of developed formulations Type de document : texte imprimé Auteurs : Majher I. Sarker, Auteur ; Wilbert Long, Auteur ; Cheng-Kung Liu, Auteur Année de publication : 2019 Article en page(s) : p. 271-278 Note générale : Bibliogr. Langues : Américain (ame) Catégories : Chlorure de sodium -- Suppression ou remplacement Cuirs et peaux -- Conservation Cuirs et peaux de bovins Formulation (Génie chimique) Hydratation Microbiologie Produits de conservation du cuir Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : The traditional wet salting technique of bovine hide curing requires 95% or more saturated brine solution which generates a huge amount of pollution and salinity in the form of total dissolved solids (TDS) and chlorides (Cl-) during leather processing. Therefore, much attention is currently placed on tanneries requiring reduction or eliminate the use of sodium chloride as a raw hide/skin preservative. For the preservation of hide it is essential to arrest microbial attack as the collagen-breaking enzymes called collagenase produced by the bacteria can cause serious degradation on hide which leads to the putrefaction of raw hide before converting them into leather. In this research effort, a number of formulas have been developed and evaluated for their efficacy in preserving bovine hide for 30 days or more. This attempt has been made to evolve a least chloride containing curing system for salinity abatement, where 35% saturated brine is used as the base solution. The developed formulations have been evaluated with better efficacy than the conventional technique on limiting bacterial growth, controlling yeast and mold during the storage period of cured hide. The alternative methods also offer quick rehydration of cured hide during leather processing and Airborne Ultrasonic scanning detects no detrimental impact on alternatively cured hides. Therefore, this new development is ideally suited for hide preservation through better protection of hide from microbial degradation and improved conservation of environment in compare to the traditional method. Note de contenu : - EXPERIMENTAL : Materials - Protocol for the alternative hide preservation - Effectiveness of the developed formulations in limiting microbial growth - Measurement of water activity (wa) and moisture content - Determination of bacterial colony count - Yeast and molds determination experiment - Airborne ultrasonic (AU) scanning - Rehydration of cured hides - Degradation assessment of cured hides - RESULTS AND DISCUSSION : Efficacy analysis of the developed formulations - Water activity and moisture content - Bacterial colony count on cured hides - Yeasts and molds colony count on cured hides - Airborne ultrasonic testing of cured hides - Rehydration anlaysis - Physical changes of the cured hides En ligne : https://drive.google.com/file/d/1X2pm9-1GO3lbEGjTfI_g-3jik5QoP7iS/view?usp=drive [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=32786 [article]

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