| Titre : |
Enzymatic hydrolysis of skin shavings for preparation of collagen hydrolysates with specified molecular weight distribution |
| Type de document : |
texte imprimé |
| Auteurs : |
Chi Yuanlong, Auteur ; Cui Min, Auteur ; Cui Xiaoju, Auteur ; Wenhua Zhang, Auteur ; Xuepin Liao, Auteur ; Bi Shi, Auteur |
| Année de publication : |
2012 |
| Article en page(s) : |
p. 16-20 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Collagène
Cuirs et peaux -- Déchets
Hydrolysats de protéines
Hydrolyse enzymatique
|
| Tags : |
'Copeaux tannerie' 'Protéase 1398' 2709' Alcalase Papaïne Pepsine 537' Trypsine 'Titration formaldehyde' Electrophorèse 'Ges dodécyl sulfate-polyacrylamide sodium' 'Ultrafiltration centrifuge' |
| Index. décimale : |
675 Technologie du cuir et de la fourrure |
| Résumé : |
Collagen hydrolysates were extracted from tannery skin shavings through enzymatic hydrolysis by using protease 1398, protease 2709, alcalase, papain, pepsin, protease 537 and trypsin, respectively. The hydrolytic degree and molecular weight distribution (MWD) of the hydrolysages were evaluated by the formaldehyde titration method, sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE), tricine-SDS-PAGE and centrifugal ultrafiltration. It was found that the MWD of the as prepared collagen hydrolysates has a close correlation with the hydrolytic degree, and greatly depends on the enzyme employed. The low molecular weight (MW) collagen hydrolysates, with relatively high hydrolytic degree, were obtained by enzymatic hydrolysis using protease 1398, protease 2709, papain and alcalase. More than 60% (mass ratio) of the fractions in these four hydrolysates were in the molecular weight range of <10kDa. The use of protease 537 produced medium-MW collagen hydrolysages where 40% of the fractionswere of 10-30kDa. The high-MW collagen hydrolysages that include about 66% fractions with molecular weights higher than 20kDa were obtained by using pepsin, accompanied by the lowest hydrolytic degree. This research provides a potential approache for molecular weight and molecular weight distribution control collagen hydrolysates by enzymatic hydrolysis ot tannery skin wastes. |
| Note de contenu : |
- MATERIALS AND METHODS : Materials - Methods - Determination of hydrolytic degree of collagen - SDS-PAGE and tricine-SDS-PAGE determination - Centrifugal ultrafiltration analysis
- RESULTS AND DISCUSSION : Hydrolytic degree analysis
- SDS-PAGE and tricine-SDS-PAGE patterns - Centrifugal ultrafiltration analysis on MWD |
| En ligne : |
https://drive.google.com/file/d/1NEe4OCKiTxbrMEX3emn2sQFAzagsFJQc/view?usp=drive [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=13549 |
in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 96, N° 1 (01-02/2012) . - p. 16-20
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