Enzymatic bacterial dehairing of bovine hide by a locally isolated strain of bacillus Licheniformis / E. H. A. Nashy in JOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC), Vol. 89, N° 6 (11-12/2005)  

[article]  inJOURNAL OF THE SOCIETY OF LEATHER TECHNOLOGISTS & CHEMISTS (JSLTC) > Vol. 89, N° 6 (11-12/2005) . - p. 242-249 Titre : Enzymatic bacterial dehairing of bovine hide by a locally isolated strain of bacillus Licheniformis Type de document : texte imprimé Auteurs : E. H. A. Nashy, Auteur ; S. A. Ismail, Auteur ; A. M. Ahmady, Auteur ; H. El-Fadaly, Auteur ; N. H. El-Sayed, Auteur Année de publication : 2005 Article en page(s) : p. 242-249 Note générale : Bibliogr. Langues : Anglais (eng) Catégories : Bacillus licheniformis Bactéries -- Identification Caractérisation Cuirs et peaux de bovins Epilage enzymatique Index. décimale : 675 Technologie du cuir et de la fourrure Résumé : Bacterial isolation and identification were realized for microbial enzymatic dehairing of animal skin. The process was optimized for the required skin quality taking into account the contact time, temperature, pH, crude enzyme concentration (%) as well as mechanical agitation. Scanning electron microscopy (SEM) was used to show the effect of bacterial enzyme on the grain surface and fibres in comparison with the chemical unhairing considering the quality of the skin. The optimization of keratinase activity was also examined. The bacterial isolates were identified as Bacillus licheniformis, (coded as D-10). The experimental results showed that the contact time of 48hr at 35°C, pH8.7, crude enzymatic concentration (v/v) 6% and shaking at 100 rev. min-1 are optimal for enzymatic dehairing of animal hide. The results obtained and the SEM results indicate the possibility of using bacterial enzymes to dehair animal skin instead of using chemical compounds, to assure good skin quality, protecting the environment and saving time. Note de contenu : - MATERIALS AND METHODS : Growth Conditions - Isolation and Identification of Proteolytic Bacteria - Preparation of standard inoculum and enzyme production - Enzyme Assay - Enzymatic dehairing by selected Bacillus licheniformis - Dehairing of skin by Na2S/Lime - RESULTS AND DISCUSSION : Screening and Selection of the most potent isolate for animal skin dehairing - Identification of the selected potent isolate - CONCLUSION : Bacterial enzyme/sulphide comparison - Characterization of the enzymatic reaction conditions - Table 1 : Shape and proteolytic potency of the bacterial isolates at 37°C for 48 hours and their effect on animal skin - Table 2 : Morphological and biochemical characteristics of bacterial isolate active in animal skin dehairing - Table 3 : Factors affecting on animal skin dehairing by enzyme En ligne : https://drive.google.com/file/d/1DHdolEkyxA7dBoVPYRDyLUKv1yvT860f/view?usp=share [...] Format de la ressource électronique : Pdf Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=39214 [article]

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