[article]
| Titre : |
Rapid determination of L-ascorbic acid content in vitamin C serums by ultra-high-performance liquid chromatography–tandem mass spectrometry |
| Type de document : |
document électronique |
| Auteurs : |
Jessica Santos Pizzo, Auteur ; Victor Hugo Maldonado Cruz, Auteur ; Carina Alexandra Rodrigues, Auteur |
| Année de publication : |
2022 |
| Article en page(s) : |
p. 131-141 |
| Note générale : |
Bibliogr. |
| Langues : |
Anglais (eng) |
| Catégories : |
Analyse quantitative (chimie)
Antioxydants
Chromatographie en phase liquide à hautes performances
Cosmétiques -- Additifs
Cosmétiques -- Analyse
Pénétration (physique)
Qualité -- Contrôle
Sérums
Spectrométrie de masse
Vitamine C
|
| Index. décimale : |
668.5 Parfums et cosmétiques |
| Résumé : |
- Objective : This study aimed to develop and validate a rapid, simple, accurate and precise analytical method for the quantification of L-AA in vitamin C serums. Moreover, the developed method was further applied to determine L-AA in eight different brands of vitamin C serums. A complementary study was also carried out to evaluate the stability of L-AA in the vitamin C serum samples after 15, 30, 45 and 60 days of storage at ambient temperature (15–35°C).
- Methods : Ultra-high-performance liquid chromatography–tandem mass spectrometry was applied.
- Results : Quantitative analyses were performed with a total chromatographic run time of 1.5 min by matrix-matched calibration, and the analytical curve was linear over the range of 1–1700 µg L−1 with a correlation coefficient of 0.9998. The limits of detection (LOD) and quantification (LOQ) were 0.3 and 1.0 µg L−1, respectively. Intra- and inter-assay precisions, expressed in terms of relative standard deviation, ranged from 0.3% and 2.2%, respectively, and recoveries in concentration levels of 1 and 5 µg L−1 were 103.9% and 101.2%, respectively. The proposed analytical method was successfully applied to determine the L-AA content in eight commercial vitamin C serum samples. The stability of the target analyte in samples stored at ambient temperature (15–35°C) was evaluated throughout 60 days with a 15-day interval between analyses. At 0 days, L-AA content in samples ranged from 1.05 to 169.91 mg L−1, which decreases over time.
- Conclusion : The proposed method could be powerful in routine analyses to ensure the quantification of L-AA in vitamin C serums since it proved to be a simple, reliable, fast, precise, accurate and sensitive analytical method. |
| Note de contenu : |
- MATERIALS AND METHODS : Standards and reagents - Ultra-high-
performance liquid chromatography conditions - Sample collection - Preparation of vitamin C samples - Method validation - Assay of commercial vitamin C serum samples - Statistical analysis
- RESULTS AND DISCUSSION : Method validation - Assay of commercial vitamin C serum samples
- Table 1 : Vitamin C serum samples from the Brazilian market with their respective prices for bottle containers of 30 ml
- Table 2 : Analytical curve parameters, the limit of detection, the limit of quantification and the matrix effect for L-AA from vitamin C serum samples
- Table 3 : Recovery, and intra-and inter-day precisions of the proposed method for determination of L-AA in vitamin C serum samples
- Table 4 : Analytical performance of chromatographic methods used for the L-ascorbic acid determination in the different sample matrices
- Table 5 : UHPLC-MS/MS determination of L-AA in vitamin C serum samples |
| DOI : |
https://doi.org/10.1111/ics.12762 |
| En ligne : |
https://drive.google.com/file/d/1ovO5r2Gs6dBBuRlrdqUldRgYeeD9QKfW/view?usp=shari [...] |
| Format de la ressource électronique : |
Pdf |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=37265 |
in INTERNATIONAL JOURNAL OF COSMETIC SCIENCE > Vol. 44, N° 1 (02/2022) . - p. 131-141
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