2 résultat(s) recherche sur le tag 'microarray''

20-O-?-D-Glucopyranosyl-20 (S)-protopanaxadiol (compound K) induces expression of hyaluronan synthase 2 gene in transformed human keratinocytes and fibroblasts and increases hyaluronan in hairless mouse skin / Sujong Kim in IFSCC MAGAZINE, Vol. 7, N° 3 (07-08-09/2004)

[article]  inIFSCC MAGAZINE > Vol. 7, N° 3 (07-08-09/2004) . - p. 189-195 Titre : 20-O-?-D-Glucopyranosyl-20 (S)-protopanaxadiol (compound K) induces expression of hyaluronan synthase 2 gene in transformed human keratinocytes and fibroblasts and increases hyaluronan in hairless mouse skin Type de document : texte imprimé Auteurs : Sujong Kim, Auteur ; Byung Young Kang, Auteur ; Si Yong Cho, Auteur ; Dae Suk Sung, Auteur ; Eiu Suk Shin, Auteur ; Hui Kyung Chang, Auteur ; Myung Hun Yeom, Auteur ; Kwang Sik Woo, Auteur ; Duk Hee Kim, Auteur ; Young Chul Sim, Auteur ; Yong Sung Lee, Auteur Année de publication : 2004 Article en page(s) : p. 189-195 Note générale : Bibliogr. Langues : Anglais (eng) Tags : 'cDNA  microarray'  '20-O-?-D-glucopyranosyle-20  (S)-protopanaxadiole'  'ginsenosides'  Hyaluronane  'Synthase  de  hyaluronane' Index. décimale : 668.5 Parfums et cosmétiques Résumé : Ginsenosides, the major active ingredient of ginseng, show a variety of biomedical efficacies such as anti-aging, anti-oxidation and anti-inflammatory activities. To understand the effects of 20-O-?-D-glucopyranosyl-20 (S)-protopanaxadiol (compound K), one of the major metabolites of ginsenosides on the skin, we assessed the expression level of approximately 100 transcripts in compound K-treated HaCaT cells using cDNA microarray analysis. Compound K treatment induced differential expression of 40 genes, which have been reported to be involved in the organization of the structure of the extracellular matrix as well as defense responses in human skin cells. One of the most interesting findings is a two-fold increase in hyaluronan synthase2 (HAS2) gene expression by compound K. We found that change in expression of HAS2 gene represents a specific response of HaCaT cells to compound K because hyaluronan synthase 1,3 was not changed by treatment with compound K. We also demonstrated that the compound K effectively induced hyaluronan synthesis in human skin cells and hairless mouse skin. A human clinical study indicated that topical application of compound K containing oil-in-water emulsion showed improvement of xerosis, wrinkle and fine lines in the aged skin. We concluded that compound K has anti-aging effects by the induction of HAS2 gene expression and following hyaluronan synthase. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10474 [article]

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Cutaneous restructuration by apple seed phytosterols : from DNA chip analysis to morphological alteration / Thomas Doering in IFSCC MAGAZINE, Vol. 7, N° 4 (10-11-12/2004)

[article]  inIFSCC MAGAZINE > Vol. 7, N° 4 (10-11-12/2004) Titre : Cutaneous restructuration by apple seed phytosterols : from DNA chip analysis to morphological alteration Type de document : texte imprimé Auteurs : Thomas Doering, Auteur ; Olaf Holtkötter, Auteur ; Kordula Schlotmann, Auteur ; Claudia Jassoy, Auteur ; Dirk Petersohn, Auteur ; Armin Wadle, Auteur ; Marianne Waldmann-Laue, Auteur Année de publication : 2004 Note générale : Bibliogr. Langues : Anglais (eng) Tags : 'Skin  equivalent'  'cDNA  microarray'  'hyaluronic  acid'  phytosterols  'anti-wrinkle'  'skin  elasticity' Index. décimale : 668.5 Parfums et cosmétiques Résumé : Plant secondary metabolites such as flavonoids, isoflavones and phytosterols have been proposed as cosmetic ingredients displaying anti-aging effects. On the cellular level, however, the activity profiles of these ingredients are only partially understood. In this study we analyzed the effects of apple seed phytosterols on age-related structural and functional parameters using cell biochemical, molecular biological and bioengineering techniques. The expression of age-related genes was studied using skin equivalents and cDNA microarrays. Incubation of skin equivalents with apple seed phytosterols had significant consequences: (i) differential regulation of a set of genes associated with keratinocyte proliferation and differentiation, (ii) stimulation of hyaluronic acid synthesis, and (iii) increase of epidermal thickness. In vivo studies revealed that apple seed phytosterols improve skin elasticity and decrease skin roughness. In conclusion, apple seed phytosterols display distinct biological effects and significantly improve the structure and function of mature skin. Permalink : https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=10481 [article]

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