[article]
| Titre : |
Biosynthesis of a UV stable pigment : prodigiosin |
| Type de document : |
texte imprimé |
| Auteurs : |
V. Natarajan, Auteur ; P. K. Kamath, Auteur |
| Année de publication : |
1995 |
| Article en page(s) : |
p. 23-33 |
| Note générale : |
Glossaire - Bibliogr. |
| Langues : |
Anglais (eng) |
| Tags : |
Biosynthèse Pigment Prodigiosine 'Serratia marcescens' 'Culture de microorganismes' Mutagenèse Optimisation 'Zinc II Ion' Mutation 'Cobalt 'Milieu culture' 'Nickel 'Elément trace' 'Fer Nutriment 'Titane 'Irradiation UV' 'Molybdène Enterobacteriaceae Bactérie |
| Index. décimale : |
667.9 Revêtements et enduits |
| Résumé : |
Pigment Prodigiosin is red maroon in colour and has a pyrrye dipyrryl methene structure. It is water insoluble and occurs membrane bound in the cells of Serratia marcescens, which is gram negative bacterium. As the parent strain of Serratia marcescens NRRL B284 was found to be unstable in its pigment synthesising ability, an attempt was therefore made to derive UV stable mutants employing UV induced mutagenesis. Out of seven mutants derived, a lone mutant designed M2 was found to be most promising both in terms of stable pigment producing characters as well as faster growth rates in select synthetic medium compared to the rest of the mutants. Studies on mutant M2 constitue optimisation of Carbon:Nitrogen ratio in the synthetic medium selected, followed by studies on feeding salts of trace cations during cell cultivation (as nutrient modifiers) to explore further (if any), scope for improving the UV stability of the pigment synthesised. The role/contribution towards UV stability by trace cations e.g. Mo++, Ti++, Fe++, Ni++, Co++ and Zn++ were investigated. Select UV stable pigments were evaluated for their performance in two types of air drying paints, with intersting results with respect to colour and gloss retention |
| Note de contenu : |
HISTORY OF PROIDOGIOSIN
- SCOPE OF CURRENT WORK
- MATERIAL AND METHODS
- EXPERIMENTAL : Culture maintenance and inoculum preparation - Screening of media for pigment production - Optimisation of carbone : nitrogen ratio for pigment synthesis - Cell harvesting, determination of biomass, pigment extraction - Chromatographic analysis of pigment - Reversion to chromogenic variants - Role of oxygen - Straint improvement through UV mutagenesis - Screening of salts of trace cations as nutrient modifiers - Pigment screening for uv stability - Prodigiosin for paint application
- RESULTS AND DISCUSSION : Reversion to chromogenic variants - UV induced mutagenesis and effect of mutation - Effect of oxyten - Effect of optimising carbon : nitrogen in synthetic medium for mutant M2 - Composition of MSM - Effect of trace cations on UV stability of pigment - Performance of prodigiosin in paints - IR interpretation |
| Permalink : |
https://e-campus.itech.fr/pmb/opac_css/index.php?lvl=notice_display&id=13665 |
in PAINTINDIA > Vol. XLV, N° 10 (10/1995) . - p. 23-33
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